公开(公告)号：US09994891B2

公开(公告)日：2018-06-12

申请号：US14350095

申请日：2012-10-11

申请人： QIAGEN GMBH

发明人： Andy Wende ,  Rainer Dahlke ,  Ralf Himmelreich ,  Thomas Rothmann ,  Michael Eberhard ,  Gerd Grosshauser ,  Markus Jeziorski ,  Hans Attig ,  Josef Drexler ,  Eva Holzer

IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/689 ,  B01L3/5027 ,  B01L3/5082 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6888

摘要： The present invention pertains inter alia to a method for analyzing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample. Furthermore, specifically adapted processing cartridges and systems are provided.

公开(公告)号：US20140287955A1

公开(公告)日：2014-09-25

申请号：US14350095

申请日：2012-10-11

申请人： QIAGEN GmbH

发明人： Andy Wende ,  Rainer Dahlke ,  Ralf Himmelreich ,  Thomas Rothmann ,  Michael Eberhard ,  Gerd Grosshauser ,  Markus Jeziorski ,  Hans Attig ,  Josef Drexler ,  Eva Holzer

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  B01L3/5027 ,  B01L3/5082 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6888

摘要： The present invention pertains inter alia to a method for analysing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample. Furthermore, specifically adapted processing cartridges and systems are provided.

摘要翻译： 本发明特别涉及用于分析包含生物分子的样品的方法，其包括以下步骤：a）裂解样品以提供裂解的样品并任选地清除裂解的样品; b）使至少一部分裂解样品与含有用于进行分析方法的试剂的干组合物接触，从而提供重构的组合物; c）使用重构的组合物进行分析方法; 或B a）使样品与裂解溶液接触，从而提供裂解混合物; b）使用裂解混合物重构含有用于进行分析方法的试剂的干组合物，从而提供重构的组合物; c）使用重构的组合物进行分析方法，其中任选地清除重构的组合物，其中在步骤b）之后，进行支持样品裂解的至少一个步骤。 此外，提供特别适合的处理盒和系统。