公开(公告)号：US20150299769A1

公开(公告)日：2015-10-22

申请号：US14441036

申请日：2013-11-07

申请人： QIAGEN GMBH

发明人： Gerd Grosshauser ,  Ralf Himmelreich

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12N15/101 ,  C12N15/1013

摘要： A method for lysing a fixed biological sample to obtain a lysate that is suitable for direct use in a subsequent nucleic acid analysis method. The method includes contacting the fixed biological sample with an aqueous lysis composition to obtain a lysis mixture, and heating the lysis mixture at ≧85° C. to obtain the lysate. Inhibitors of the subsequent nucleic acid analysis method are depleted by contacting the fixed biological sample in the first step with at least one compound which prevents or reduces the inhibition of the subsequent nucleic acid analysis method, and/or binding inhibitors to a solid support having an anionic surface. The method is rapid, efficient, does not require the use of chaotropic salts and does not require a prior purification of nucleic acids prior to performing the subsequent analysis method. A portion of the obtained lysate can be used directly, for example, in an amplification reaction.

摘要翻译： 一种裂解固定的生物样品以获得适于直接用于随后的核酸分析方法的裂解物的方法。 该方法包括使固定生物样品与含水裂解组合物接触以获得裂解混合物，并在≥85℃加热裂解混合物以获得裂解物。 接下来的核酸分析方法的抑制剂通过使第一步中的固定生物样品与至少一种预防或减少后续核酸分析方法的抑制的化合物接触，和/或将抑制剂结合到具有 阴离子表面。 该方法快速，有效，不需要使用离液盐，并且在进行后续分析方法之前不需要先前纯化核酸。 所获得的溶胞产物的一部分可以直接使用，例如扩增反应。

公开(公告)号：US09822404B2

公开(公告)日：2017-11-21

申请号：US14441112

申请日：2013-11-07

申请人： QIAGEN GMBH

发明人： Gerd Grosshauser ,  Andy Wende ,  Ralf Himmelreich

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q2527/125 ,  C12Q2549/125

摘要： A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay.

公开(公告)号：US20150292000A1

公开(公告)日：2015-10-15

申请号：US14441112

申请日：2013-11-07

申请人： QIAGEN GMBH

发明人： Gerd Grosshauser ,  Andy Wende ,  Rlaf Himmelreich

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q2527/125 ,  C12Q2549/125

摘要： A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay.

摘要翻译： 一种用于确定裂解物是否含有足够的用于核酸扩增反应的生物样本材料的方法，其包括在至少一种抑制扩增反应的化合物的存在下制备裂解物，如果在制备溶胞产物期间存在不足的生物样品材料，则 如果在制备溶胞产物期间存在足够的生物样品材料，使裂解物进行扩增反应并分析扩增反应的结果，则不抑制扩增反应。 由于在扩增反应中存在化合物，如果在制备溶胞产物期间存在不足的生物样品材料，则不能获得扩增信号，但如果在制备溶胞产物期间存在足够的生物样品材料，则获得扩增信号。 本发明允许可靠地鉴定由于不足的样品材料进行扩增测定而发生的假阴性。

公开(公告)号：US09994891B2

公开(公告)日：2018-06-12

申请号：US14350095

申请日：2012-10-11

申请人： QIAGEN GMBH

发明人： Andy Wende ,  Rainer Dahlke ,  Ralf Himmelreich ,  Thomas Rothmann ,  Michael Eberhard ,  Gerd Grosshauser ,  Markus Jeziorski ,  Hans Attig ,  Josef Drexler ,  Eva Holzer

IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/689 ,  B01L3/5027 ,  B01L3/5082 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6888

摘要： The present invention pertains inter alia to a method for analyzing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample. Furthermore, specifically adapted processing cartridges and systems are provided.

公开(公告)号：US20140287955A1

公开(公告)日：2014-09-25

申请号：US14350095

申请日：2012-10-11

申请人： QIAGEN GmbH

发明人： Andy Wende ,  Rainer Dahlke ,  Ralf Himmelreich ,  Thomas Rothmann ,  Michael Eberhard ,  Gerd Grosshauser ,  Markus Jeziorski ,  Hans Attig ,  Josef Drexler ,  Eva Holzer

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  B01L3/5027 ,  B01L3/5082 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6888

摘要： The present invention pertains inter alia to a method for analysing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample. Furthermore, specifically adapted processing cartridges and systems are provided.

摘要翻译： 本发明特别涉及用于分析包含生物分子的样品的方法，其包括以下步骤：a）裂解样品以提供裂解的样品并任选地清除裂解的样品; b）使至少一部分裂解样品与含有用于进行分析方法的试剂的干组合物接触，从而提供重构的组合物; c）使用重构的组合物进行分析方法; 或B a）使样品与裂解溶液接触，从而提供裂解混合物; b）使用裂解混合物重构含有用于进行分析方法的试剂的干组合物，从而提供重构的组合物; c）使用重构的组合物进行分析方法，其中任选地清除重构的组合物，其中在步骤b）之后，进行支持样品裂解的至少一个步骤。 此外，提供特别适合的处理盒和系统。