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19 条记录 (耗时 0.016 秒)

    Method for analyzing aurka expression
    1.
    发明授权
    Method for analyzing aurka expression 有权

    公开(公告)号：US11840734B2

    公开(公告)日：2023-12-12

    申请号：US16494949

    申请日：2018-03-01

    申请人： Qiagen GmbH

    发明人： Siegfried Hauch ,  Markus Sprenger-Haussels

    IPC分类号： C12Q1/6886

    CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/158

    摘要： Provided is a method for analysing the expression of one or more biomarker RNA molecules, comprising (A) isolating RNA from circulating tumor cells obtained from a subject, determining the expression of at least one biomarker RNA molecule in the isolated RNA and providing an expression profile based on the results; (B) isolating RNA from extracellular vesicles obtained from the subject, determining the expression of at least one biomarker RNA molecule in the isolated RNA and providing an expression profile based on the results; and (C) using the expression profiles determined in (A) and determined in (B) for a combined analysis of the results. Such combined analysis of the CTC and EV expression profiles enhances the prognostic and predictive value of the obtained results and can provide valuable diagnostic, prognostic and/or predictive information. The present method can thus be used as improved diagnostic, prognostic and/or predictive aid in the management of cancer patients. It can be used to support the diagnosis, prognosis or to choose the most appropriate treatment for cancer patients.

    Rapid method for isolating extracellular nucleic acids
    4.
    发明授权
    Rapid method for isolating extracellular nucleic acids 有权

    公开(公告)号：US11021736B2

    公开(公告)日：2021-06-01

    申请号：US16204332

    申请日：2018-11-29

    申请人： QIAGEN GmbH

    发明人： Martin Horlitz ,  Annette Nocon ,  Markus Sprenger-Haussels ,  Peter Grünefeld ,  Christoph Erbacher

    IPC分类号： C12N15/10 ,  C12Q1/6806

    摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps:
    a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture;
    b. separating the solid phase with the bound extracellular nucleic acids;
    c. optionally washing the extracellular nucleic acids;
    d. optionally eluting extracellular nucleic acids from the solid phase.
    The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

    RAPID METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS
    7.
    发明申请
    RAPID METHOD FOR ISOLATING EXTRACELLULAR NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20190226007A1

    公开(公告)日：2019-07-25

    申请号：US16204332

    申请日：2018-11-29

    申请人： QIAGEN GmbH

    发明人： Martin Horlitz ,  Annette Nocon ,  Markus Sprenger-Haussels ,  Peter Grünefeld ,  Christoph Erbacher

    IPC分类号： C12Q1/6806 ,  C12N15/10

    摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps:a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture;b. separating the solid phase with the bound extracellular nucleic acids;c. optionally washing the extracellular nucleic acids;d. optionally eluting extracellular nucleic acids from the solid phase.The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

    Rapid method for isolating extracellular nucleic acids
    8.
    发明授权
    Rapid method for isolating extracellular nucleic acids 有权

    公开(公告)号：US10184145B2

    公开(公告)日：2019-01-22

    申请号：US14347452

    申请日：2012-09-25

    申请人： Qiagen GmbH

    发明人： Martin Horlitz ,  Annette Nocon ,  Markus Sprenger-Haussels ,  Peter Grünefeld ,  Christoph Erbacher

    IPC分类号： C12N15/10 ,  C12Q1/6806

    摘要： The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; separating the solid phase with the bound extracellular nucleic acids; optionally washing the extracellular nucleic acids; optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.

    METHOD OF DETERMINING THE PRESENCE OR ABSENCE OF A TARGET NUCLEIC ACID IN A CELL SAMPLE
    10.
    发明申请
    METHOD OF DETERMINING THE PRESENCE OR ABSENCE OF A TARGET NUCLEIC ACID IN A CELL SAMPLE 审中-公开
    确定细胞样品中目标核酸存在或不存在的方法

    公开(公告)号：US20150218653A1

    公开(公告)日：2015-08-06

    申请号：US14424230

    申请日：2013-08-16

    申请人： QIAGEN GmbH

    发明人： Markus Sprenger-Haussels ,  Christian Kupfer ,  Peer Schatz

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12N15/10

    CPC分类号： C12Q1/6886 ,  C12N15/101 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/708 ,  C12Q2563/143 ,  C12Q2600/118 ,  C12Q2600/158

    摘要： A method of determining the presence or absence of a target nucleic acid in a cell sample, said method comprising: a) contacting a surface comprising anion exchange moieties with the sample under conditions suitable to induce binding between the cells and said surface; b) separating the surface with the bound cells from the remaining sample to collect the cells; c) releasing nucleic acids from the cells and d) generating a hybrid between the target nucleic acid and a probe specific for the target nucleic acid e) detecting the presence or absence of the hybrid. The present invention is provides a rapid and automatable method, wherein cells, such as epithelial cells originating from cervical swab samples, are collected from the surrounding liquid medium, such as a liquid based cytology medium, by binding them to an anion exchange surface. The cells bind directly with high affinity and quick kinetics to the anion exchange surface which preferably is provided by magnetic particles carrying anion exchange moieties. The cells that are bound to the anion exchange surface can be easily separated from the surrounding medium and can be directly resuspended in a composition that is suitable for a subsequent hybrid capturing assay which is performed in step d) to detect e. g. pathogen nucleic acids such as HPV nucleic acids.

    摘要翻译： 一种确定细胞样品中靶核酸的存在或不存在的方法，所述方法包括：a）在适于诱导细胞与所述表面之间结合的条件下，使含有阴离子交换部分的表面与样品接触; b）将表面与剩余样品中的结合细胞分离以收集细胞; c）从细胞中释放核酸，以及d）在靶核酸和靶核酸特异的探针之间产生杂交体e）检测杂交体的存在或不存在。 本发明提供一种快速且可自动化的方法，其中通过将它们结合到阴离子交换表面上，从周围的液体介质如基于液体的细胞学介质中收集源自子宫颈拭子样品的细胞，例如上皮细胞。 细胞直接以高亲和力和快速动力学结合阴离子交换表面，其优选由携带阴离子交换部分的磁性颗粒提供。 与阴离子交换表面结合的细胞可以容易地与周围的培养基分离，并且可以直接重新悬浮在组合物中，该组合物适用于在步骤d）中进行的随后的杂交捕获测定，以检测e。 G。 病原体核酸如HPV核酸。