公开(公告)号：US09574186B2

公开(公告)日：2017-02-21

申请号：US14697843

申请日：2015-04-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Werner Hoelke ,  Johann-Peter Thalhofer ,  Antje Liehre ,  Markus Weber

IPC: C12N9/54 ,  C12N9/96

CPC classification number: C12N9/54 ,  C12N9/96 ,  C12Y304/24027

Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.

Abstract translation: 本发明涉及在水溶液中趋于不稳定的蛋白水解酶嗜热溶菌酶。 本发明提供了增加溶解的嗜热溶菌酶在水溶液中的稳定性的方法和组合物。 将含有嗜热菌蛋白酶和一种或多种盐的溶血素酶，粗嗜热菌蛋白酶或冻干物质与低盐浓度的水性缓冲液接触，形成第一溶液。 随后，加入固体形式的另外的盐并解离，由此形成稳定形式的包含嗜热菌蛋白酶的第二溶液。

公开(公告)号：US20150337282A1

公开(公告)日：2015-11-26

申请号：US14697843

申请日：2015-04-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Werner Hoelke ,  Johann-Peter Thalhofer ,  Antje Liehre ,  Markus Weber

IPC: C12N9/54

CPC classification number: C12N9/54 ,  C12N9/96 ,  C12Y304/24027

Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.

Abstract translation: 本发明涉及在水溶液中趋于不稳定的蛋白水解酶嗜热溶菌酶。 本发明提供了增加溶解的嗜热溶菌酶在水溶液中的稳定性的方法和组合物。 将含有嗜热菌蛋白酶和一种或多种盐的溶血素酶，粗嗜热菌蛋白酶或冻干物质与低盐浓度的水性缓冲液接触，形成第一溶液。 随后，加入固体形式的另外的盐并解离，由此形成稳定形式的包含嗜热菌蛋白酶的第二溶液。