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公开(公告)号：US20230052545A1
公开(公告)日：2023-02-16
申请号：US17822587
申请日：2022-08-26
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Michaela Fischer , Johann-Peter Thalhofer , Markus Weber
Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.
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公开(公告)号：US20170247679A1
公开(公告)日：2017-08-31
申请号：US15595126
申请日：2017-05-15
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Michaela Fischer , Johann-Peter Thalhofer , Markus Weber
CPC classification number: C12N9/96 , C12N9/52 , C12N9/54 , C12N9/6491 , C12Y304/24003 , C12Y304/24027
Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.
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Patent Agency Ranking
公开(公告)号：US09574186B2
公开(公告)日：2017-02-21
申请号：US14697843
申请日：2015-04-28
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Johann-Peter Thalhofer , Antje Liehre , Markus Weber
CPC classification number: C12N9/54 , C12N9/96 , C12Y304/24027
Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.
Abstract translation: 本发明涉及在水溶液中趋于不稳定的蛋白水解酶嗜热溶菌酶。 本发明提供了增加溶解的嗜热溶菌酶在水溶液中的稳定性的方法和组合物。 将含有嗜热菌蛋白酶和一种或多种盐的溶血素酶，粗嗜热菌蛋白酶或冻干物质与低盐浓度的水性缓冲液接触，形成第一溶液。 随后，加入固体形式的另外的盐并解离，由此形成稳定形式的包含嗜热菌蛋白酶的第二溶液。
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公开(公告)号：US20150337282A1
公开(公告)日：2015-11-26
申请号：US14697843
申请日：2015-04-28
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Johann-Peter Thalhofer , Antje Liehre , Markus Weber
IPC: C12N9/54
CPC classification number: C12N9/54 , C12N9/96 , C12Y304/24027
Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.
Abstract translation: 本发明涉及在水溶液中趋于不稳定的蛋白水解酶嗜热溶菌酶。 本发明提供了增加溶解的嗜热溶菌酶在水溶液中的稳定性的方法和组合物。 将含有嗜热菌蛋白酶和一种或多种盐的溶血素酶，粗嗜热菌蛋白酶或冻干物质与低盐浓度的水性缓冲液接触，形成第一溶液。 随后，加入固体形式的另外的盐并解离，由此形成稳定形式的包含嗜热菌蛋白酶的第二溶液。
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公开(公告)号：US11459556B2
公开(公告)日：2022-10-04
申请号：US16865487
申请日：2020-05-04
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Michaela Fischer , Johann-Peter Thalhofer , Markus Weber
Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.
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公开(公告)号：US20200263160A1
公开(公告)日：2020-08-20
申请号：US16865487
申请日：2020-05-04
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Michaela Fischer , Johann-Peter Thalhofer , Markus Weber
Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.
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公开(公告)号：US20160319268A1
公开(公告)日：2016-11-03
申请号：US14699299
申请日：2015-04-29
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Werner Hoelke , Michaela Fischer , Johann-Peter Thalhofer , Markus Weber
CPC classification number: C12N9/96 , C12N9/52 , C12N9/54 , C12N9/6491 , C12Y304/24003 , C12Y304/24027
Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.
Abstract translation: 描述了含有蛋白水解酶的组合物，特别是冻干物，以及用于制备组合物的方法。 通常，这些组合物含有一种或多种具有胶原酶活性的蛋白酶和中性蛋白酶，例如嗜热菌蛋白酶。 组合物不含乙酸盐。 令人惊奇的是，这种组合物可以比现有技术的冻干蛋白酶混合物更快地溶解在水中。
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