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    MANUFACTURING METHOD FOR 1,4-BUTANEDIOL, MICROBE, AND GENE
    1.
    发明申请
    MANUFACTURING METHOD FOR 1,4-BUTANEDIOL, MICROBE, AND GENE 有权
    1,4-丁二醇，微生物和基因的制备方法

    公开(公告)号：US20150376657A1

    公开(公告)日：2015-12-31

    申请号：US14440116

    申请日：2013-11-28

    Applicant: SHOWA DENKO K.K.

    Inventor： Hirobumi AOKI ,  Yuzuru KOKIDO ,  Yoko HASHIMOTO ,  Tadashi YONEDA

    IPC: C12P7/18 ,  C12N9/90 ,  C12N9/88 ,  C12N9/10 ,  C12N9/04

    CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01035 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y203/01009 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y402/01119 ,  C12Y402/0112 ,  C12Y503/03003

    Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.

    Abstract translation: 通过使用微生物和/或其培养物通过乙酰辅酶A，乙酰乙酰辅酶A，3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A制备1,4-丁二醇的方法，其中 1,4-丁二醇的制造方法包括（a）具有序列号1的碱基序列的基因中的任一基因，（b）具有缺失一个或多个碱基的碱基序列，取代基， 或以序列号1的碱基序列加入，其中所述基因具有相对于序列号1的碱基序列具有大于或等于90％的同一性的碱基序列，和（c）与基因杂交的基因 其具有与在严格条件下具有序列号1所述的碱基序列的基因互补的碱基序列，并且包括（d）具有序列号为2〜9的碱基序列的基因中的任何一个或多个基因，（e） 具有碱基序列使得一个或多个碱基被缺失，取代或添加的基因 序列号2〜9的碱基序列，其中所述基因具有相对于其原始碱基序列具有大于或等于90％的同一性的碱基序列，和（f）与具有与基因互补的碱基序列的基因杂交的基因， 在严格条件下具有序列号2到9的碱基序列。

    METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE
    2.
    发明申请
    METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE 审中-公开
    制备1,4-丁二醇和微生物的方法

    公开(公告)号：US20150353962A1

    公开(公告)日：2015-12-10

    申请号：US14440117

    申请日：2013-09-20

    Applicant: SHOWA DENKO K.K.

    Inventor： Hirobumi AOKI ,  Yuzuru KOKIDO ,  Yoko HASHIMOTO ,  Tadashi YONEDA

    IPC: C12P7/18 ,  C12N9/02

    CPC classification number: C12N9/0008 ,  C12P7/16 ,  C12P7/18 ,  C12Y102/0101 ,  Y02E50/10

    Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acyl-CoA reductase, via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA in this order, wherein the reactivity of the acyl-CoA reductase to 4-hydroxybutyryl CoA is greater than or equal to 0.05 times of the reactivity of the acyl-CoA reductase to 3-hydroxybutyryl-CoA.

    Abstract translation: 通过使用酰基辅酶A还原酶的酶反应体系，通过3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A按顺序制备1,4-丁二醇的方法，使用微生物和/或其培养物 其中酰基辅酶A还原酶对4-羟基丁酰辅酶A的反应性大于或等于酰基辅酶A还原酶对3-羟基丁酰辅酶A的反应性的0.05倍。

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