公开(公告)号：US20210047682A1

公开(公告)日：2021-02-18

申请号：US16591232

申请日：2019-10-02

Applicant: Seagate Technology LLC

Inventor： Gemma MENDONSA ,  Riyan A. MENDONSA ,  Krishnan SUBRAMANIAN

IPC: C12Q1/6869 ,  G01N33/487 ,  G01N27/72

Abstract: Method of utilizing a nanochannel in combination with at least one magnetic sensor for detecting (e.g., identifying) molecules, cells, and other analytes. Particularly, the method includes bringing molecules, labeled with magnetic nanoparticles (MNPs), in close proximity to the magnetic sensor to identify the molecules via an output signal from the magnetic sensor. The method is particularly suited for identifying nucleotides of DNA and RNA strands.

公开(公告)号：US20210349054A1

公开(公告)日：2021-11-11

申请号：US16870580

申请日：2020-05-08

Applicant: Seagate Technology LLC

Inventor： Gemma MENDONSA ,  Tim RAUSCH ,  Riyan Alex MENDONSA

IPC: G01N27/447 ,  C12N15/10

Abstract: Systems and methods for identifying DNA strand size and purifying the DNA based on strand size using electrophoresis. The methods include moving, via voltage, a plurality of DNA strands through a separation gel from an inlet of a capillary or passage to either a first outlet or a second outlet dependent on the DNA strand length. In some implementations, the system is a capillary electrophoresis system. In other implementations, the system is a microfluidic lab-on-a-chip.

公开(公告)号：US20220090157A1

公开(公告)日：2022-03-24

申请号：US17031664

申请日：2020-09-24

Applicant: Seagate Technology LLC

Inventor： Gemma MENDONSA ,  Riyan Alex MENDONSA ,  Tim RAUSCH

IPC: C12P19/34

Abstract: Methods of writing data to a DNA strand by inserting data-encoding oligos or symbols into a DNA backbone. One particular method of synthesizing a DNA strand encoding data includes cleaving a DNA backbone into multiple segments, and pasting a plurality of data-encoding oligo symbols between the multiple segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, resulting in the DNA strand encoding data comprising alternating segments and symbols.

公开(公告)号：US20210309991A1

公开(公告)日：2021-10-07

申请号：US17348642

申请日：2021-06-15

Applicant: Seagate Technology LLC

Inventor： Tim RAUSCH ,  Walter R EPPLER ,  Gemma MENDONSA

IPC: C12N15/10 ,  C40B60/14 ,  B01L3/00 ,  G16B50/30 ,  G06F12/02 ,  G11C13/02

Abstract: A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first end and a second end and a second linker of the pair having a first end and a second end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

公开(公告)号：US20210054364A1

公开(公告)日：2021-02-25

申请号：US16857006

申请日：2020-04-23

Applicant: Seagate Technology LLC

Inventor： Tim RAUSCH ,  Walter R EPPLER ,  Gemma MENDONSA

IPC: C12N15/10 ,  B01L3/00 ,  C40B60/14

Abstract: A microfluidic lab-on-a-chip system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The lab-on-a-chip has a fluidic platform with a plurality of arrays operably connected to a voltage source and a controller for the voltage source, a set of first inlets operably connected to the fluidic platform, each first inlet for one DNA symbol from a DNA symbol library, a set of second inlets operably connected to the fluidic platform, each second inlet for one DNA linker from a DNA linker library, and a mixing area operably connected to the fluidic platform and to the plurality of first inlets and the plurality of second inlets.

公开(公告)号：US20240282374A1

公开(公告)日：2024-08-22

申请号：US18638432

申请日：2024-04-17

Applicant: Seagate Technology LLC

Inventor： Gemma MENDONSA ,  Riyan Alex MENDONSA ,  Tim RAUSCH

IPC: G11C13/02 ,  C12P19/34

CPC classification number: G11C13/02 ,  C12P19/34

Abstract: Methods of writing data to a DNA strand by inserting data-encoding oligos or symbols into a DNA backbone. One particular method of synthesizing a DNA strand encoding data includes cleaving a DNA backbone into multiple segments, and pasting a plurality of data-encoding oligo symbols between the multiple segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, resulting in the DNA strand encoding data comprising alternating segments and symbols.

公开(公告)号：US20210054368A1

公开(公告)日：2021-02-25

申请号：US16856947

申请日：2020-04-23

Applicant: Seagate Technology LLC

Inventor： Tim RAUSCH ,  Walter R EPPLER ,  Gemma MENDONSA

IPC: C12N15/10 ,  G16B50/30 ,  G06F12/02

Abstract: A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first overhanging end and a second overhanging end and a second linker of the pair having a first overhanging end and a second overhanging end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

公开(公告)号：US20210047681A1

公开(公告)日：2021-02-18

申请号：US16591162

申请日：2019-10-02

Applicant: Seagate Technology LLC

Inventor： Gemma MENDONSA ,  Riyan A. MENDONSA ,  Krishnan SUBRAMANIAN

IPC: C12Q1/6869 ,  C12Q1/6876

Abstract: Methods of DNA sequencing from a template strand via DNA polymerase immobilized on a magnetic sensor. The methods include forming a complementary strand by the DNA polymerase from individual magnetic nanoparticle (MNP)-labeled nucleotides, and obtaining a signal from the magnetic sensor to identify the MNP and thus the nucleotide. The process can be repeated until the entire complementary strand is formed and identified.