公开(公告)号：US20240371622A1

公开(公告)日：2024-11-07

申请号：US18610570

申请日：2024-03-20

Applicant: Standard BioTools Canada Inc. ,  University of Ottawa

Inventor： Paul Corkum ,  Alexander V. Loboda

IPC: H01J49/04 ,  G01N33/68 ,  G02B21/33 ,  H01J37/20 ,  H01J37/28 ,  H01J49/00 ,  H01J49/02 ,  H01J49/06 ,  H01J49/10 ,  H01J49/14 ,  H01J49/16 ,  H01J49/40

Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC®) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.

公开(公告)号：US20250044571A1

公开(公告)日：2025-02-06

申请号：US18887633

申请日：2024-09-17

Applicant: Standard BioTools Canada Inc.

Inventor： Daaf Sandkuijl ,  Alexander V. Loboda ,  Adam Carew ,  Khashayar Askarpour

IPC: G02B21/24 ,  G01N1/04 ,  G01N33/543 ,  G02B21/00 ,  G02B21/26 ,  H01J49/00 ,  H01J49/10

Abstract: We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.

公开(公告)号：US20240369567A1

公开(公告)日：2024-11-07

申请号：US18644592

申请日：2024-04-24

Applicant: Standard BioTools Canada Inc.

Inventor： Alexander V. Loboda ,  Olga Ornatsky ,  Vladimir Baranov ,  Nikita Zabinyakov ,  Anastasia Mavropoulos ,  Daniel Majonis ,  Bedilu Allo ,  Taunia Closson

IPC: G01N33/68 ,  C07K1/13 ,  G01N1/28

Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. Embodiments include a method for quantifying one or more analytes within a sample. The method may include providing the sample. One or more analytes may be immobilised to a mass cytometry sample carrier. The sample may have been labelled with one or more mass-tagged SBPs including one or more labelling atoms. The method may include performing mass cytometry on the sample to determine a level of the one or more labelling atoms, where the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes to quantify the analytes.