-
公开(公告)号:US20070254292A1
公开(公告)日:2007-11-01
申请号:US11660549
申请日:2005-08-18
申请人: Tadashi Fukasawa , Hiroshi Kurokawa
发明人: Tadashi Fukasawa , Hiroshi Kurokawa
CPC分类号: C12N15/1006 , C12Q1/6806 , C12Q2563/137 , C12Q2527/119 , C12Q2523/308
摘要: A method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and having a pH of 12 or higher with the surface of the solid phase substrate on which divalent metal ions are immobilized, and a method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and a magnesium compound, of which pH is adjusted to be 12 or higher, with the surface of the solid phase substrate.
摘要翻译: 一种用于捕获固相底物表面上的核酸的方法,其包括使含有核酸并且具有12或更高pH值的样品与其上固定有二价金属离子的固相底物的表面接触的步骤, 以及用于在固相底物的表面上捕获核酸的方法,其包括将pH调节至12或更高的含有核酸和镁化合物的样品与固相的表面接触的步骤 基质。
-
公开(公告)号:US07615347B2
公开(公告)日:2009-11-10
申请号:US11660549
申请日:2005-08-18
申请人: Tadashi Fukasawa , Hiroshi Kurokawa
发明人: Tadashi Fukasawa , Hiroshi Kurokawa
CPC分类号: C12N15/1006 , C12Q1/6806 , C12Q2563/137 , C12Q2527/119 , C12Q2523/308
摘要: A method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and having a pH of 12 or higher with the surface of the solid phase substrate on which divalent metal ions are immobilized, and a method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and a magnesium compound, of which pH is adjusted to be 12 or higher, with the surface of the solid phase substrate.
摘要翻译: 一种用于捕获固相底物表面上的核酸的方法,其包括使含有核酸并且具有12或更高pH值的样品与其上固定有二价金属离子的固相底物的表面接触的步骤, 以及用于在固相底物的表面上捕获核酸的方法,其包括将pH调节至12或更高的含有核酸和镁化合物的样品与固相的表面接触的步骤 基质。
-