公开(公告)号：US5100557A

公开(公告)日：1992-03-31

申请号：US443149

申请日：1989-11-30

申请人： Taro Nogami ,  Hironori Kaji ,  Kaoru Hagiya ,  Katsuo Tsukada ,  Yoshinori Takata

发明人： Taro Nogami ,  Hironori Kaji ,  Kaoru Hagiya ,  Katsuo Tsukada ,  Yoshinori Takata

IPC分类号： B01D15/08 ,  G01N30/24 ,  G01N30/44 ,  G01N30/82

CPC分类号： G01N30/44 ,  G01N30/82 ,  G01N30/24

摘要： A liquid chromatography system and a method of utilizing the same, comprising a plurality of separated-component vessels for collecting components eluted in a separation column, a flowing system conduit for component extraction through which the components separated in the separation column are individually delivered to the separated-component vessels. The system further comprises a flowing system conduit for sample introduction through which any of the separated components collected in the plurality of separated-component vessels is selectively drawn from its vessel and introduced to the upstream side of the separation column. The present invention allows a plurality of components in a sample of a mixture to be individual collected, and also allows any of the collected components to be re-separated selectively and automatically.

摘要翻译： 一种液相色谱系统及其应用方法，包括用于收集在分离塔中洗脱的组分的多个分离组分容器，用于组分萃取的流动系统管道，分离塔中分离的组分通过其分别输送到 分离容器。 该系统还包括用于样品引入的流动系统管道，通过该流动系统管道，收集在多个分离组分容器中的任何分离的组分从其容器中选择性地拉出并被引入分离塔的上游侧。 本发明允许混合物样品中的多个组分被单独收集，并且还允许任何收集的组分被选择性地和自动地重新分离。

公开(公告)号：US4233156A

公开(公告)日：1980-11-11

申请号：US18334

申请日：1979-03-07

申请人： Katsuo Tsukada ,  Sadabumi Ohnuma

发明人： Katsuo Tsukada ,  Sadabumi Ohnuma

IPC分类号： B01D15/08 ,  G01N30/32 ,  G01N30/34

CPC分类号： B01D15/08 ,  G01N30/32 ,  G01N30/34 ,  B01D15/14 ,  B01D15/163 ,  B01D15/166 ,  G01N2030/326

摘要： A plurality of liquid mobile phases stored in a plurality of reservoirs respectively are filled and pumped by a dual pump driven by a stepping motor, through valves complementarily subjected to on-off control by a gradient programmer. The dual pump has two pistons in contact with the periphery of a cam in opposition to each other at 180 degrees, which pistons fill and pump the liquid mobile phases by linear reciprocation along the contour of the cam with the rotation of the cam. The radius of the cam linearly increases in the range from 0 to 180 degrees and linearly decreases in the range from 180 to 360 degrees at the same rate as when it increases. An electric signal is produced from a timing device during a period from the rotational positions of the cam reaches 0 and 180 degrees to a predetermined rotational positions. The liquid mobile phase delivered from the dual pump is supplied to a pressure transducer, and after passing through it, supplied to a column through an injector for injecting a specimen. The components into which the specimen is separated are detected by a detector. When the timing device detects that the cam is positioned in intervals from 0 and 180 degree to a predetermined angle therefrom, the motor control circuit drives the stepping motor at high speed during period from a time which the time derivative of the back pressure signal from the pressure transducer reaches value smaller than a predetermined value to a time it reaches value larger than the predetermined value.

摘要翻译： 存储在多个储存器中的多个液体流动相分别通过由步进电机驱动的双泵通过由梯度编程器互补地进行开关控制的阀来填充和泵送。 双泵具有两个与凸轮的周边相接触的活塞，180度相互相对，活塞通过凸轮的旋转沿着凸轮的轮廓线性往复运动而填充和泵送液体流动相。 凸轮的半径在0至180度的范围内线性地增加，并且以与增加的速度相同的速率在180度至360度的范围内线性地减小。 在从凸轮的旋转位置到达0和180度到预定旋转位置的期间，从定时装置产生电信号。 从双泵输送的液体流动相被供应到压力传感器，并且在通过之后，通过用于注射样品的注射器供应到塔。 通过检测器检测样品分离到的部件。 当定时装置检测到凸轮位于从0到180度到预定角度的间隔时，电动机控制电路在时间段内驱动步进电动机，时间从时间导数 压力传感器达到小于预定值的值至达到大于预定值的值的时间。