公开(公告)号：US20240264133A1

公开(公告)日：2024-08-08

申请号：US18163406

申请日：2023-02-02

申请人： Yamazen Corporation

发明人： Kihachiro OKURA

IPC分类号： G01N30/95 ,  B01D15/16 ,  G01N30/90

CPC分类号： G01N30/95 ,  B01D15/166 ,  G01N30/90

摘要： Provided is a separation chromatography supporting device capable of predicting, using a result of thin-layer chromatography or column chromatography, a result of liquid chromatography for a wide range of compounds with high accuracy and setting an optimum separation condition. The separation chromatography supporting device includes a calculation formula acquisition unit, and provides information related to separation in column chromatography based on an actually measured value of an Rf value when a mixed solvent having a mixing ratio B of two specific types of solvents measured by thin-layer chromatography or liquid chromatography is used. The calculation formula acquisition unit acquires a1 in a relational formula of







Rf
=



a
1

⁢
B

+

b
1






(
1
)









or a2 in a relational formula of












log
⁢

k
′


=



a
2

⁢
log
⁢
B

+

b
2






(
2
)












(


where
⁢

retention
⁢

ratio
:


k
′


=


(


t
R

-

t
0


)

/

t
0








(
3
)










solvent ratio: B) based on B and Rf that are actually measured values as one measurement result by TLC.

公开(公告)号：US11981934B2

公开(公告)日：2024-05-14

申请号：US16958974

申请日：2018-12-27

申请人： TAKEDA PHARMACEUTICAL COMPANY LIMITED

发明人： Christian Fiedler ,  Meinhard Hasslacher ,  Jadranka Koehn

IPC分类号： C07K14/435 ,  A61K38/00 ,  B01D15/16 ,  B01D15/36 ,  B01D15/38 ,  B01D15/42 ,  C07K14/705 ,  C07K16/28 ,  C12N7/00 ,  G01N33/569

CPC分类号： C12N7/00 ,  B01D15/166 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/426 ,  G01N33/56983 ,  C12N2750/14151 ,  G01N2333/075

摘要： Provided herein are methods of producing an adeno-associated virus (AAV) product and methods of purifying adeno-associated virus. AAV is loaded onto an affinity resin, wash steps are undertaken, and AAV is eluted from the affinity resin. Various buffers are disclosed for use in the wash steps and elution.

公开(公告)号：US20240140970A1

公开(公告)日：2024-05-02

申请号：US18003402

申请日：2021-11-26

申请人： ASCENTAWITS PHARMACEUTICALS, LTD.

发明人： Meizhen Ruan ,  Xiaohong Cai ,  Jianxin Duan ,  Donald T Jung ,  Anrong Li ,  Teng Meng ,  Lin Sun ,  Bing Li

IPC分类号： C07F9/564 ,  B01D15/16 ,  B01D15/42 ,  C07B59/00 ,  G01N30/06 ,  G01N30/16 ,  G01N30/72

CPC分类号： C07F9/564 ,  B01D15/166 ,  B01D15/426 ,  C07B59/002 ,  G01N30/06 ,  G01N30/16 ,  G01N30/7233 ,  C07B2200/05 ,  G01N2030/027

摘要： Described is a deuterated compound, and preparation method and use thereof The deuterated compound I has a structure as shown in Formula (I), wherein A is H or D, and at least one of eight As is D; M is H or an alkali metal, an alkaline earth metal, or an ammonium radical. The present invention provides use of the deuterated compound I as an internal standard for measuring the content of a metabolite II in a biological sample, wherein the metabolite II has a structure as shown in a Formula (II); wherein A is H; M is H or an alkali metal, an alkaline earth metal, or an ammonium radical. The present invention uses the deuterated compound I as an internal standard to quantitatively analysis the content of metabolite II at lower content in biological samples, which can not only meet the requirements for the lower limit of quantitation, but also meet the requirements for DMPK studies in clinical trials.

公开(公告)号：US11959894B2

公开(公告)日：2024-04-16

申请号：US16971216

申请日：2019-02-22

申请人： SILCOTEK CORP.

发明人： Jesse Bischof ,  Lucas D. Patterson ,  Gary Barone ,  Min Yuan ,  David A. Smith

IPC分类号： G01N30/56 ,  B01D15/16 ,  B01D15/20 ,  B01D15/22 ,  B01D15/38 ,  G01N30/30 ,  G01N30/36 ,  B01J20/32 ,  G01N30/02 ,  G01N30/60

CPC分类号： G01N30/56 ,  B01D15/161 ,  B01D15/163 ,  B01D15/166 ,  B01D15/206 ,  B01D15/22 ,  B01D15/3828 ,  G01N30/30 ,  G01N30/36 ,  B01J20/3265 ,  G01N2030/027 ,  G01N2030/567 ,  G01N30/60

摘要： LC techniques are disclosed. The LC technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150 degree Celsius, pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone.

公开(公告)号：US20240009594A1

公开(公告)日：2024-01-11

申请号：US18474000

申请日：2023-09-25

申请人： Cytiva Sweden AB

发明人： Bjorn Markus Olovsson

IPC分类号： B01D15/16 ,  G01N30/32 ,  G01N30/34 ,  B01F23/40 ,  B01F25/31 ,  B01F35/21 ,  B01F35/221 ,  B01F35/71 ,  B01D15/22

CPC分类号： B01D15/166 ,  G01N30/32 ,  G01N30/34 ,  B01F23/49 ,  B01F25/31 ,  B01F35/2132 ,  B01F35/2133 ,  B01F35/2211 ,  B01F35/7176 ,  B01D15/22 ,  G01N2030/347 ,  B01D15/16 ,  G01N30/8658

摘要： A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

公开(公告)号：US11732245B2

公开(公告)日：2023-08-22

申请号：US17379359

申请日：2021-07-19

申请人： The Trustees of the University of Pennsylvania

发明人： Martin Lock ,  Mauricio Alvira

IPC分类号： C12N7/02 ,  C12N15/86 ,  B01D15/38 ,  B01J20/281 ,  C12N15/864 ,  G01N21/33 ,  B01J41/05 ,  B01D15/16 ,  B01D15/36 ,  B01J41/20

CPC分类号： C12N7/02 ,  B01D15/3804 ,  B01J20/281 ,  C12N15/86 ,  C12N15/8645 ,  G01N21/33 ,  B01D15/166 ,  B01D15/363 ,  B01J41/05 ,  B01J41/20 ,  C12N2750/14143 ,  C12N2750/14151

摘要： A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

公开(公告)号：US20230243788A1

公开(公告)日：2023-08-03

申请号：US18084415

申请日：2022-12-19

申请人： Agilent Technologies, Inc.

发明人： Christian Hirn ,  Christoph Keppler

IPC分类号： G01N30/74 ,  G01N30/14 ,  B01D15/14 ,  B01D15/16 ,  B01D15/24 ,  B01D19/00

CPC分类号： G01N30/74 ,  G01N30/14 ,  B01D15/14 ,  B01D15/166 ,  B01D15/247 ,  B01D19/0068 ,  G01N2030/027

摘要： A lamp-housing assembly, for a detector of a sample separation apparatus for separating a fluidic sample, includes a lamp seat, a lamp insertable into the lamp seat, and a lamp cap mountable on the lamp seat and on the inserted lamp. The lamp seat, lamp and lamp cap are matched with respect to each other so that, by inserting the lamp into the lamp seat and by mounting the lamp cap on the lamp seat and on the inserted lamp, the lamp is axially and radially aligned and electrically and thermally coupled with the lamp seat and the lamp cap.

公开(公告)号：US10005006B2

公开(公告)日：2018-06-26

申请号：US14661793

申请日：2015-03-18

申请人： DIONEX SOFTRON GMBH

发明人： Hermann Hochgraeber ,  Thomas Wachinger

IPC分类号： G01N30/34 ,  B01D15/16 ,  B01D15/14

CPC分类号： B01D15/166 ,  G01N30/34

摘要： The invention relates to a method for setting a gradient delay volume GDV of a liquid chromatography system for a chromatography run in liquid chromatography, in particular a high-performance liquid chromatography system, in which a desired value GDVtarget of a gradient delay volume of the liquid chromatography system is ascertained or predefined and, if the value GDVtarget deviates from a specific fixed value GDVactual of a liquid chromatography system, this value GDVtarget is set in a range 0≤ΔGDV=GDVtarget−GDVactual≤Vmax of a volume of a volume adjustment device 5. Furthermore, the invention relates to an automatic sampler for carrying out such a method.

公开(公告)号：US20170218011A1

公开(公告)日：2017-08-03

申请号：US15455956

申请日：2017-03-10

申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

发明人： Peter Stanley Gagnon

IPC分类号： C07K1/16 ,  C12N7/00 ,  B01D15/36 ,  C07K14/75 ,  B01D15/34 ,  C07K16/00 ,  C07K14/755

CPC分类号： C07K14/75 ,  B01D15/08 ,  B01D15/166 ,  B01D15/1807 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3885 ,  C07K1/16 ,  C07K14/755 ,  C07K16/00 ,  C07K16/065 ,  C12N7/00 ,  C12N2795/00 ,  C12N2795/14151

摘要： Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.

公开(公告)号：US20170212087A1

公开(公告)日：2017-07-27

申请号：US15530578

申请日：2015-08-18

申请人： John M RIVIELLO

发明人： John M. Riviello

IPC分类号： G01N30/96 ,  B01D15/16 ,  B01D15/36

CPC分类号： G01N30/96 ,  B01D15/166 ,  B01D15/22 ,  B01D15/36 ,  B01D15/361

摘要： A chambered ion reflux device for ion chromatography which electrolytically produces an eluent from a water pumped phase, suppresses the eluent and recovers (refluxes) the eluent ions for reuse. In the chambered ion reflux device, the eluent never passes through the electrode chambers. Since the eluent ions are refluxed and the eluent is produced electrolytically, the chambered ion reflux device can be used for isocratic or gradient ion chromatography. A chambered ion reflux device which integrates an electrolytic ion removal chamber is also disclosed.