公开(公告)号：US20060127992A1

公开(公告)日：2006-06-15

申请号：US10528881

申请日：2003-09-16

申请人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Yoshie Nagahashi ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Yoshie Nagahashi ,  Masaaki Tazoe

IPC分类号： C12P17/12 ,  C12N1/21

CPC分类号： C12N9/0008 ,  C12N9/93 ,  C12P17/12

摘要： Disclosed is a recombinant microorganism of the genus Sinorhizobium which is transformed with a vector containing pyridoxol 5′-phophate synthase gene and D-erythrose 4-phosphate dehydrogenase gene, and being capable of producing vitamin B6.

摘要翻译： 公开了用含有吡哆醇5'-磷酸合酶基因和D-赤藓糖-4-磷酸脱氢酶基因的载体转化的Sinorhizobium属的重组微生物，并且能够产生维生素B6。

公开(公告)号：US07598066B2

公开(公告)日：2009-10-06

申请号：US10528845

申请日：2003-09-23

申请人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

IPC分类号： C12N9/16 ,  C12N15/70 ,  C12N1/21 ,  C12N1/16 ,  C12P21/06 ,  C12P17/00 ,  C12P17/12 ,  C07H21/04

CPC分类号： C12N9/16 ,  C12P17/12

摘要： Disclosed is an isolated DNA encoding vitamin B6 phosphate phosphatase selected from the group consisting of: (a) a DNA sequence represented in SEQ ID NO:9; (b) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and hybridizes under standard conditions to the DNA sequence defined in (a) or a fragment of thereof; (c) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity, wherein said polypeptide is at least 70% identical to the amino acid sequence represented in SEQ ID NO:10; (d) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and is at least 70% identical to the DNA sequence represented in SEQ ID NO:9; (e) a degenerate DNA sequence of any one of (a) to (c).

摘要翻译： 公开了编码选自以下的维生素B6磷酸磷酸酶的分离DNA：（a）SEQ ID NO：9所示的DNA序列; （b）编码具有维生素B6磷酸酯酶活性的多肽的DNA序列，并在标准条件下与（a）中定义的DNA序列或其片段杂交; （c）编码具有维生素B6磷酸酯酶活性的多肽的DNA序列，其中所述多肽与SEQ ID NO：10所示氨基酸序列至少70％相同; （d）编码具有维生素B6磷酸酯酶活性并与SEQ ID NO：9所示DNA序列至少70％相同的多肽的DNA序列; （e）（a）至（c）中任一项的简并DNA序列。

公开(公告)号：US20060263862A1

公开(公告)日：2006-11-23

申请号：US10528845

申请日：2003-09-23

申请人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Yoshie Nagahashi ,  Masaaki Tazoe

IPC分类号： C12P17/00 ,  C12N9/16 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/16 ,  C12P17/12

摘要： Disclosed is an isolated DNA encoding vitamin B6 phosphate phosphatase selected from the group consisting of: (a) a DNA sequence represented in SEQ ID NO:9; (b) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and hybridizes under standard conditions to the DNA sequence defined in (a) or a fragment of thereof; (c) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity, wherein said polypeptide is at least 70% identical to the amino acid sequence represented in SEQ ID NO: 10; (d) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and is at least 70% identical to the DNA sequence represented in SEQ ID NO:9; (e) a degenerate DNA sequence of any one of (a) to (c).

摘要翻译： 公开了编码选自以下的维生素B 6磷酸磷酸酶的分离DNA：（a）SEQ ID NO：9所示的DNA序列; （b）编码具有维生素B 6磷酸磷酸酶活性的多肽的DNA序列，并在标准条件下与（a）中定义的DNA序列或其片段杂交; （c）编码具有维生素B 6磷酸磷酸酶活性的多肽的DNA序列，其中所述多肽与SEQ ID NO：10所示氨基酸序列至少70％相同; （d）编码具有维生素B 6磷酸磷酸酶活性的多肽的DNA序列，与SEQ ID NO：9所示的DNA序列至少70％相同; （e）（a）至（c）中任一项的简并DNA序列。

公开(公告)号：US20060216798A1

公开(公告)日：2006-09-28

申请号：US10528892

申请日：2003-09-16

申请人： Tatsuo Hoshino ,  Yoichiro Nagatani ,  Keiko Ichikawa ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Yoichiro Nagatani ,  Keiko Ichikawa ,  Masaaki Tazoe

IPC分类号： C12P17/12 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12R1/41 ,  C12P17/12

摘要： Disclosed is a mutant of a recombinant microorganism of the genus Sinorhizobium capable of producing vitamin B6 having a recombinant plasmid with pdxJ gene that acquired histidine requirement of glycine resistance, or its combination thereof.

摘要翻译： 公开了能够产生具有获得甘氨酸抗性的组氨酸要求的pdxJ基因的重组质粒或其组合的具有能够产生维生素B 6的中华根瘤菌属的重组微生物的突变体。

公开(公告)号：US07371547B2

公开(公告)日：2008-05-13

申请号：US10528892

申请日：2003-09-16

申请人： Tatsuo Hoshino ,  Yoichiro Nagatani ,  Keiko Ichikawa ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Yoichiro Nagatani ,  Keiko Ichikawa ,  Masaaki Tazoe

IPC分类号： C12P17/12 ,  C12N9/02 ,  C12N1/20 ,  C07K1/00 ,  C07H21/04

CPC分类号： C12R1/41 ,  C12P17/12

摘要： Disclosed is a mutant of a recombinant microorganism of the genus Sinorhizobium capable of producing vitamin B6 having a recombinant plasmid with pdxJ gene that acquired histidine requirement of glycine resistance, or its combination thereof.

摘要翻译： 公开了能够产生具有获得甘氨酸抗性的组氨酸要求的pdxJ基因的重组质粒或其组合的具有能够产生维生素B 6的中华根瘤菌属的重组微生物的突变体。

公开(公告)号：US5766894A

公开(公告)日：1998-06-16

申请号：US709068

申请日：1996-09-06

申请人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

IPC分类号： C12P17/12 ,  C12R1/41

CPC分类号： C12P17/12

摘要： A process for producting vitamin B.sub.6 comprises cultivating a microorganism belonging to the genus Rhizobium and being capable of producing vitamin B.sub.6 in a culture medium under aerobic conditions, and separating the resulting vitamin B.sub.6 from the fermentation broth. As well as containing assimilable carbon sources, digestible nitrogen sources, inorganic salts and other nutrients necessary for the growth of the microorganism, the culture medium preferably additionally contains pyruvate, D-glyceraldehyde, glycolaldehyde, glycine, 1-deoxy-D-threo-pentulose, 4-hydroxy-L-threonine or a mixture thereof. This process affords high yields of vitamin B.sub.6, a vitamin essential for the nutrition of animals, plants and microorganisms and useful as a medicine and in foodstuffs.

摘要翻译： 生产维生素B6的方法包括培养属于根瘤菌属的微生物并且在需氧条件下能够在培养基中产生维生素B6，并将所得到的维生素B6与发酵液分离。 除了含有可吸收的碳源，可消化的氮源，无机盐和微生物生长所必需的其他营养物之外，培养基优选另外含有丙酮酸，D-甘油醛，乙二醇醛，甘氨酸，1-脱氧-D-苏糖 - ，4-羟基-L-苏氨酸或其混合物。 该方法提供高产量的维生素B6，维生素B6是维持动物，植物和微生物营养所必需的维生素，可用作药物和食品。

公开(公告)号：US07432089B2

公开(公告)日：2008-10-07

申请号：US10528844

申请日：2003-09-25

申请人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

IPC分类号： C12P17/06 ,  C12P17/12 ,  C12Q1/68 ,  C12N21/06 ,  C12N15/70 ,  C12N1/20 ,  C12N1/00 ,  C12N9/04 ,  C07H21/04

CPC分类号： C12N9/0006

摘要： The present invention relates to a DNA encoding a novel flavin adenine dinucleotide (FAD)-dependent D-erythronate 4-phosphate (EN4P) dehydrogenase originated from Sinorhizobium meliloti, which is involved in vitamin B6 biosynthesis, and a recombinant microorganism transformed with a vector having the DNA. It also relates to a process for production of vitamin B6 by using the recombinant microorganism. “Vitamin B6” as used in this invention includes pyridoxol (PN), pyridoxal and pyridoxamine. Vitamin B6 is a vitamin indispensable to human beings or other animals, and is used as a raw material of medicines or as feed additives.

摘要翻译： 本发明涉及编码参与维生素B6生物合成的源自中华根瘤菌中华根瘤菌的新型黄素腺嘌呤二核苷酸（FAD） - 依赖性D-赤藓酸苷4-磷酸（EN4P）脱氢酶的DNA，以及用载体转化的重组微生物， DNA。 它还涉及通过使用重组微生物生产维生素B6的方法。 本发明中使用的“维生素B6”包括吡哆醛（PN），吡哆醛和吡哆胺。 维生素B6是人类或其他动物不可或缺的维生素，用作药物的原料或饲料添加剂。

公开(公告)号：US20060228785A1

公开(公告)日：2006-10-12

申请号：US10528891

申请日：2003-09-18

申请人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

IPC分类号： C12P19/42 ,  C12N1/21

CPC分类号： C12N9/0004 ,  C12P17/12

摘要： Disclosed is a recombinant microorganism being capable of producing vitamin B6, wherein said microorganism carries extra genes which code for an enzyme combination selected from: i) erythrose 4-phosphate dehydrogenase and 1-deoxy-D-xylulose 5-phosphate synthase; ii) erythrose 4-phosphate dehydrogenase and pyridoxol 5′-phosphate synthase; and iii) erythrose 4-phosphate dehydrogenase, 1-deoxy-D-xylulose 5-phosphate synthase and pyridoxol 5′-phosphate synthase.

摘要翻译： 公开了能够产生维生素B6的重组微生物，其中所述微生物携带编码选自以下的酶组合的额外基因：i）赤藓糖-4-磷酸脱氢酶和1-脱氧-D-木酮糖-5-磷酸合酶; ii）赤藓糖-4-磷酸脱氢酶和吡哆醇5'-磷酸合酶; 和iii）赤藓糖-4-磷酸脱氢酶，1-脱氧-D-木酮糖-5-磷酸合成酶和吡哆醇5'-磷酸合酶。

公开(公告)号：US06984510B2

公开(公告)日：2006-01-10

申请号：US10481099

申请日：2002-06-14

申请人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

发明人： Tatsuo Hoshino ,  Keiko Ichikawa ,  Masaaki Tazoe

IPC分类号： C12N9/12 ,  C12N9/00 ,  C12P17/00 ,  C12P1/00

CPC分类号： C12P17/12 ,  C12N9/16

摘要： A purified vitamin B6-phosphate phosphatase (VB6PP), having the following physico-chemical properties: a) Molecular weight: 29,000±5,000 (consisting of a monomer having a molecular weight of 29,000±5,000); b) Co-factor: Mn2+, Mg2+, Co2+, Sn2+, or Ni2+; c) Substrate specificity: active on pyridoxol 5′-phosphate, pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate; d) Optimum temperature: 30–400° C. at pH 7.5; and e) Optimum pH: 7.0–8.0 is disclosed. Also disclosed are a process for producing VB6PP from a microorganism belonging to the genus Sinorhizobium capable of producing VB6PP, a process for producing vitamin B6 from vitamin B6-phosphate (VB6P) utilizing VB6PP, and a cell-free extract of a microorganism belonging to the genus Sinorhizobium capable of producing VB6PP.

摘要翻译： 具有以下物理化学性质的纯化的维生素B 6 - 磷酸磷酸酶（VB6PP）：a）分子量：29,000±5,000（由分子量为29,000±5,000的单体组成） b）共因子：Mn 2+，Mg 2+，Co 2+，Sn 2+， 或Ni 2+; c）底物特异性：对吡哆醇5'-磷酸，吡哆醛5'-磷酸和吡哆胺5'-磷酸活性; d）最适温度：30-400℃，pH7.5; 和e）最佳pH：7.0-8.0。 还公开了从属于能产生VB6PP的中华根瘤菌属的微生物生产VB6PP的方法，从维生素B 6磷酸酯（VB6P）生产维生素B 6的方法 ），以及能够产生VB6PP的属于中华根瘤菌属的微生物的无细胞提取物。

公开(公告)号：US06723544B2

公开(公告)日：2004-04-20

申请号：US10033078

申请日：2001-12-27

申请人： Yasuhiro Furuichi ,  Tatsuo Hoshino ,  Hitoshi Kimura ,  Tatsuya Kiyasu ,  Yoshie Nagahashi

发明人： Yasuhiro Furuichi ,  Tatsuo Hoshino ,  Hitoshi Kimura ,  Tatsuya Kiyasu ,  Yoshie Nagahashi

IPC分类号： C12N910

CPC分类号： C12N15/52 ,  A23L33/15 ,  A23V2002/00 ,  C12P17/185 ,  A23V2250/70

摘要： The present invention relates to the production process of biotin by fermentation using a genetically engineered microorganism, and DNA sequences and vectors to be used in such process.