-
1.发明申请Methods of culturing, storing, and inducing differentiation in cells, instrument for use in the methods, method of using the instrument, and medical biomaterial 审中-公开培养，储存和诱导细胞分化的方法，方法中使用的仪器，使用仪器的方法和医用生物材料公开(公告)号：US20070020756A1
公开(公告)日：2007-01-25
申请号：US11529829
申请日：2006-09-29
摘要： The present invention provides methods of culturing or storing cells for a prolonged period while suppressing a loss of functions of the cells during storage, by applying centrifugal force or pressure such as hydraulic pressure to the cells to form compact bodies, particularly aggregates, in which a state of high contact or a high contact frequency is maintained between the cells, and then culturing, storing or inducing differentiation in the cells in this aggregate state.
摘要翻译： 本发明提供长时间培养或储存细胞的方法，同时通过向细胞施加离心力或压力如液压来形成紧凑体，特别是聚集体，同时抑制储存期间细胞功能的损失，其中 在细胞之间维持高接触或高接触频率的状态，然后在该聚集状态下在细胞中培养，储存或诱导分化。
-
公开(公告)号：US20060110369A1
公开(公告)日：2006-05-25
申请号：US10525707
申请日：2003-08-28
CPC分类号： A61L27/3804
摘要： To provide a cell-filled device that is suitable for use in, for example, an implantable or circulation type hybrid artificial organ. In a cell-filled device including hollow fiber membranes whose hollow portions are filled with cells, the hollow fiber membranes have modified cross sections, and a cell aggregate provided in each of the hollow portions has cells formed into two or more layers in arbitrary directions, provided that the distance from an arbitrary point of the cell aggregate to the nearest inner wall of hollow fiber membrane is less than 75 μm. This cell-filled device enables effective use of cells without the necrosis thereof. Further, according to the present invention, there is provided a method of manufacturing the cell-filled device.
摘要翻译： 提供适用于例如可植入或循环型混合人造器官的细胞填充装置。 在包括中空纤维膜填充有细胞的中空纤维膜的电池填充装置中，中空纤维膜具有改变的横截面，并且设置在每个中空部分中的细胞集合体具有在任意方向上形成两层或更多层的单元， 假设从细胞聚集体的任意点到中空纤维膜的最近内壁的距离小于75μm。 该细胞填充装置能够有效地利用细胞而没有坏死。 此外，根据本发明，提供一种制造电池填充装置的方法。
-
公开(公告)号：US07695958B2
公开(公告)日：2010-04-13
申请号：US10525707
申请日：2003-08-28
CPC分类号： A61L27/3804
摘要： To provide a cell-filled device that is suitable for use in, for example, an implantable or circulation type hybrid artificial organ. In a cell-filled device including hollow fiber membranes whose hollow portions are filled with cells, the hollow fiber membranes have modified cross sections, and a cell aggregate provided in each of the hollow portions has cells formed into two or more layers in arbitrary directions, provided that the distance from an arbitrary point of the cell aggregate to the nearest inner wall of hollow fiber membrane is less than 75 μm. This cell-filled device enables effective use of cells without the necrosis thereof. Further, according to the present invention, there is provided a method of manufacturing the cell-filled device.
摘要翻译： 提供适用于例如可植入或循环型混合人造器官的细胞填充装置。 在包括中空纤维膜填充有细胞的中空纤维膜的电池填充装置中，中空纤维膜具有改变的横截面，并且设置在每个中空部分中的细胞集合体具有在任意方向上形成两层或更多层的单元， 条件是从细胞聚集体的任意点到中空纤维膜的最近内壁的距离小于75μm。 该细胞填充装置能够有效地利用细胞而没有坏死。 此外，根据本发明，提供一种制造电池填充装置的方法。
-
公开(公告)号：US06284451B1
公开(公告)日：2001-09-04
申请号：US09648027
申请日：2000-08-25
IPC分类号： A61F204
CPC分类号： B01D63/02 , B01D2313/14 , C12M21/08 , C12M25/10
摘要： The present invention provides a module for cell culture comprising a structure comprising hollow fibers and at least two spacers, wherein the spacers have small pores regularly arranged therein and the hollow fibers pass through the small pores and are arranged regularly at a very small distance, a hybrid artificial liver module wherein hepatocytes are immobilized in this module, and a method of cell culture wherein cells are immobilized in the lumen or extra-fiber space of the module of this module by the use of a centrifugal force and wherein the immobilized cells are cultured. The present invention also provides a method for cell culture wherein hepatocytes are cultured in a culture medium containing DMEM as a basal medium.
摘要翻译： 本发明提供了一种用于细胞培养的模块，其包括包括中空纤维和至少两个间隔物的结构，其中间隔物具有规则排列的小孔，并且中空纤维通过小孔并且以非常小的距离规则地排列， 其中肝细胞被固定在该模块中的混合人造肝组件和细胞培养方法，其中通过使用离心力将细胞固定在该组件的模块的内腔或纤维外空间中，并且其中固定的细胞被培养 。 本发明还提供一种细胞培养方法，其中在含有作为基础培养基的DMEM的培养基中培养肝细胞。
-
-
-
搜索结果
4 条记录 (耗时 0.02 秒)