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1.发明授权Compositions and polyeptides related to type 1 diacylgycerol O-acyltransferase (DGAT) 有权与1型二酰基甘油O-酰基转移酶（DGAT）相关的组合物和多肽公开(公告)号：US08748576B2
公开(公告)日：2014-06-10
申请号：US13299763
申请日：2011-11-18
申请人： William B. Allen , Bo Shen , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant, for marker-assisted selection of a plant, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物的油含量增加和/或油酸含量增加和/或油酸/亚油酸比例增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于操作植物的油和/或油酸含量和/或油酸/亚油酸比例，用于植物的标记辅助选择和标记物的方法 - 高油和/或高油酸性状的辅助育种。
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2.发明授权Method of identifying maize plants having increased oil content using quantitative trait locus 6 (QTL6) 有权使用数量性状位点6（QTL6）鉴定具有增加的油含量的玉米植物的方法公开(公告)号：US08084208B2
公开(公告)日：2011-12-27
申请号：US12875574
申请日：2010-09-03
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的用于操纵植物或植物部分的油和/或油酸含量和/或油酸/亚油酸比例的方法，用于标记辅助选择 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。
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3.发明申请COMPOSITIONS RELATED TO THE QUANTITATIVE TRAIT LOCUS 6 (QTL6) IN MAIZE AND METHODS OF USE 有权与量化条件6（QTL6）相关的组合物及其使用方法公开(公告)号：US20110252499A1
公开(公告)日：2011-10-13
申请号：US13157068
申请日：2011-06-09
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： A01H1/04 , C12N9/1029 , C12N15/8247 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172 , Y10T436/104165
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。
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4.发明授权Identifying a high oil phenotype in maize by detecting a marker locus genetically linked with a QTL6 region 有权通过检测与QTL6区域遗传连锁的标记位点，识别玉米高油表型公开(公告)号：US07981677B2
公开(公告)日：2011-07-19
申请号：US11680906
申请日：2007-03-01
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： A01H1/04 , C12N9/1029 , C12N15/8247 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172 , Y10T436/104165
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。
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5.发明授权Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods of use 有权与玉米的数量性状基因座6（QTL6）和使用方法有关的成分公开(公告)号：US07812216B2
公开(公告)日：2010-10-12
申请号：US11680922
申请日：2007-03-01
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或增加的油酸含量和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及用于高油的标记辅助育种 和/或高油酸性状。
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6.发明授权Methods of producing maize plants having a high oil phenotype by detecting a marker locus genetically linked with a QTL6 region 有权通过检测与QTL6区域遗传连锁的标记位点来生产具有高油表型的玉米植物的方法公开(公告)号：US08319010B2
公开(公告)日：2012-11-27
申请号：US13157068
申请日：2011-06-09
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： A01H1/04 , C12N9/1029 , C12N15/8247 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172 , Y10T436/104165
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。
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7.发明申请COMPOSITIONS RELATED TO THE QUANTITATIVE TRAIT LOCUS 6 (QTL6) IN MAIZE AND METHODS OF USE 有权与量化条件6（QTL6）相关的组合物及其使用方法公开(公告)号：US20120102596A1
公开(公告)日：2012-04-26
申请号：US13299763
申请日：2011-11-18
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant, for marker-assisted selection of a plant, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物的油含量增加和/或油酸含量增加和/或油酸/亚油酸比例增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于操作植物的油和/或油酸含量和/或油酸/亚油酸比例，用于植物的标记辅助选择和标记物的方法 - 高油和/或高油酸性状的辅助育种。
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8.发明申请COMPOSITIONS RELATED TO THE QUANTITATIVE TRAIT LOCUS 6 (QTL6) IN MAIZE AND METHODS OF USE 有权与量化条件6（QTL6）相关的组合物及其使用方法公开(公告)号：US20110041207A1
公开(公告)日：2011-02-17
申请号：US12875574
申请日：2010-09-03
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的用于操纵植物或植物部分的油和/或油酸含量和/或油酸/亚油酸比例的方法，用于标记辅助选择 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。
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公开(公告)号：US08383901B2
公开(公告)日：2013-02-26
申请号：US12555426
申请日：2009-09-08
申请人： William B. Allen , Bo Shen , Peizhong Zheng
发明人： William B. Allen , Bo Shen , Peizhong Zheng
CPC分类号： C12N15/8247
摘要： Methods are provided for altering plant characteristics by introducing into plants, isolated nucleic acid molecules that can be used to produce transgenic plants characterized by altered oil levels within the seed. Also provided are isolated nucleic acids that encode AP2 domain transcription factor proteins and fragments thereof, vectors capable of expressing such nucleic acid molecules, host cells containing such vectors, and polypeptides encoded by such nucleic acids.
摘要翻译： 提供了通过向植物中引入植物特征的方法，所述分离的核酸分子可用于产生以种子内改变的油含量为特征的转基因植物。 还提供了编码AP2结构域转录因子蛋白及其片段，能够表达该核酸分子的载体，含有该载体的宿主细胞和由该核酸编码的多肽的分离的核酸。
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公开(公告)号：US20100242138A1
公开(公告)日：2010-09-23
申请号：US12555426
申请日：2009-09-08
申请人： William B. Allen , Bo Shen , Peizhong Zheng
发明人： William B. Allen , Bo Shen , Peizhong Zheng
CPC分类号： C12N15/8247
摘要： Methods are provided for altering plant characteristics by introducing into plants, isolated nucleic acid molecules that can be used to produce transgenic plants characterized by altered oil levels within the seed. Also provided are isolated nucleic acids that encode AP2 domain transcription factor proteins and fragments thereof, vectors capable of expressing such nucleic acid molecules, host cells containing such vectors, and polypeptides encoded by such nucleic acids.
摘要翻译： 提供了通过向植物中引入植物特征的方法，所述分离的核酸分子可用于产生以种子内改变的油含量为特征的转基因植物。 还提供了编码AP2结构域转录因子蛋白及其片段，能够表达该核酸分子的载体，含有该载体的宿主细胞和由该核酸编码的多肽的分离的核酸。
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