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    Identifying a high oil phenotype in maize by detecting a marker locus genetically linked with a QTL6 region
    1.
    发明授权
    Identifying a high oil phenotype in maize by detecting a marker locus genetically linked with a QTL6 region 有权
    通过检测与QTL6区域遗传连锁的标记位点，识别玉米高油表型

    公开(公告)号：US07981677B2

    公开(公告)日：2011-07-19

    申请号：US11680906

    申请日：2007-03-01

    申请人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

    发明人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  G01N33/92

    CPC分类号： A01H1/04 ,  C12N9/1029 ,  C12N15/8247 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172 ,  Y10T436/104165

    摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.

    摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。

    Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods of use
    4.
    发明授权
    Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods of use 有权
    与玉米的数量性状基因座6（QTL6）和使用方法有关的成分

    公开(公告)号：US07812216B2

    公开(公告)日：2010-10-12

    申请号：US11680922

    申请日：2007-03-01

    申请人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

    发明人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

    IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/82

    CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172

    摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.

    摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或增加的油酸含量和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及用于高油的标记辅助育种 和/或高油酸性状。

    Enzymatic methods for modulating the levels of organic sulfur compounds in plants
    7.
    发明授权
    Enzymatic methods for modulating the levels of organic sulfur compounds in plants 有权
    用于调节植物中有机硫化合物水平的酶法

    公开(公告)号：US07560623B2

    公开(公告)日：2009-07-14

    申请号：US11365135

    申请日：2006-03-01

    申请人： Mitchell C. Tarczynski ,  Changjiang Li ,  Bo Shen

    发明人： Mitchell C. Tarczynski ,  Changjiang Li ,  Bo Shen

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8243 ,  C12N15/8242

    摘要： Methods for modulating levels of at least one organic sulfur compound in plants are provided. Also provided are plants and seeds produced by the methods. The methods comprise stably transforming a plant with a DNA construct encoding a cystathionine gamma synthase enzyme, a serine acetyl transferase enzyme and/or other sulfur assimilating enzymes capable of altering the level of at least one organic sulfur compound.

    摘要翻译： 提供了调节植物中至少一种有机硫化合物水平的方法。 还提供了通过这些方法生产的植物和种子。 所述方法包括用编码胱硫醚γ-合成酶的DNA构建体，丝氨酸乙酰转移酶和/或能够改变至少一种有机硫化合物的水平的其它硫同化酶稳定转化植物。