公开(公告)号：US10590467B2

公开(公告)日：2020-03-17

申请号：US15303741

申请日：2015-04-16

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido Amit ,  David Lara-Astiaso ,  Meital Gury

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6816 ,  G01N33/53

Abstract: A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising: (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety; (b) isolating the complexes on a solid support; (c) labeling the DNA of the complexes, wherein the labeling distinguishes between the complexes of the first of the at least two samples and the complexes of the second of the at least two samples; (d) pooling the at least two samples of complexes; and (e) isolating the complexes using an agent which specifically binds to the DNA binding moiety; and (f) analyzing the DNA of the complexes.

公开(公告)号：US11485971B2

公开(公告)日：2022-11-01

申请号：US16330774

申请日：2017-09-14

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido Amit ,  Diego Jaitin ,  David Lara-Astiaso ,  Assaf Weiner ,  Ido Yofe

IPC: C12N15/79 ,  C07H21/04 ,  C12N15/11 ,  C12N15/63 ,  C12N15/10 ,  C12N9/22 ,  C12Q1/6883 ,  C12N15/85 ,  C07H21/02

Abstract: An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.