公开(公告)号：US20240229121A1

公开(公告)日：2024-07-11

申请号：US18561284

申请日：2023-03-21

Applicant: ZHEJIANG LAB

Inventor： PEIXIANG MA ,  MINGTIAN ZHONG ,  LU DANG ,  BAICHENG HUANG

IPC: C12Q1/6851 ,  C12Q1/6823

CPC classification number: C12Q1/6851 ,  C12Q1/6823 ,  C12Q2600/178

Abstract: The present invention discloses a microRNA detection method and kit for rapid detection of microRNA nucleic acids in biological and environmental samples, including microRNA tailing and a Cpf1 detection system suitable for rapid detection. The present invention is the first to use a combination of microRNA tailing and Cpf1 detection to detect microRNAs, with the advantages of high sensitivity, strong specificity, short time consumption, high throughput, direct interpretation by the naked eye, no dependence on large-scale experimental equipment and the like. These advantages make the detection method developed by the present invention convenient for rapid detection, and identification and diagnosis of microRNAs in biological and environmental samples at a clinical front line.

公开(公告)号：US20250002882A1

公开(公告)日：2025-01-02

申请号：US18658938

申请日：2024-05-08

Applicant: ZHEJIANG LAB

Inventor： JIN TANG ,  JUNCHAO SHI ,  YUANYUAN FENG ,  PEIXIANG MA ,  XINGXU HUANG

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/63

Abstract: The present invention discloses a Cpf 1 protein and its use in gene editing, which relates to the field of biotechnology. The present invention provides a novel Cas12a nuclease named as the Cas12a-68 protease, which has a wider PAM recognition range than traditional nucleases and is capable of recognizing targets of various genes. When editing nucleic acid fragments, using the new nuclease of the present invention is capable of overcoming the shortcomings of the traditional Cas12a and increasing the PAM recognition range. These advantages make the present invention more suitable for editing a wider range of nucleic acid fragments and lay a foundation for gene editing applications.