公开(公告)号：US20200283853A1

公开(公告)日：2020-09-10

申请号：US15930267

申请日：2020-05-12

Applicant: Mayo Foundation for Medical Education and Research ,  EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： David A. Ahlquist ,  John B. Kisiel ,  William R. Taylor ,  Tracy C. Yab ,  Douglas W. Mahoney ,  Graham P. Lidgard ,  Hatim T. Allawi

IPC: C12Q1/6886

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as pancreatic and colorectal cancer.

公开(公告)号：US20200224278A1

公开(公告)日：2020-07-16

申请号：US16665731

申请日：2019-10-28

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim T. Allawi ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6881

Abstract: Provided herein is technology relating compositions and methods for detecting tissue cell-specific DNA, such as epithelial cell-specific DNA, in blood or blood products from a subject. The technology also relates to use of tissue cell-specific DNAs as internal controls for methylation assays.

公开(公告)号：US10704081B2

公开(公告)日：2020-07-07

申请号：US15335096

申请日：2016-10-26

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Graham P. Lidgard ,  Hatim Allawi

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6837

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US10450614B2

公开(公告)日：2019-10-22

申请号：US15987793

申请日：2018-05-23

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12P19/34 ,  C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6881

Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.

公开(公告)号：US10385406B2

公开(公告)日：2019-08-20

申请号：US15471337

申请日：2017-03-28

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Maria Giakoumopoulos ,  David A. Ahlquist ,  William R. Taylor ,  Douglas Mahoney

IPC: C12Q1/6886 ,  C12Q1/6806 ,  A61K31/713

Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

公开(公告)号：US20190161805A1

公开(公告)日：2019-05-30

申请号：US16202935

申请日：2018-11-28

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  Douglas W. Mahoney ,  Tracy C. Yab ,  John B. Kisiel ,  Hatim T. Allawi ,  Graham P. Lidgard ,  Michael W. Kaiser

IPC: C12Q1/6886 ,  C12Q1/6853 ,  G16H50/30 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12Q1/6837

Abstract: Provided herein is technology for breast cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of breast cancer.

公开(公告)号：US20190137498A1

公开(公告)日：2019-05-09

申请号：US16182854

申请日：2018-11-07

Applicant: Exact Sciences Development Company, LLC

Inventor： Jeanne Ohrnberger ,  Sharat Singh ,  Jim Arthurs ,  David Esposito

IPC: G01N33/574 ,  G16H50/20 ,  G06F17/15

Abstract: Disclosed herein are methods and composition of improving accuracy of cancer detection and enhancing therapeutic outcomes. The compositions, methods and systems described herein facilitate assessing risk assessment of cancer.

公开(公告)号：US20190055609A1

公开(公告)日：2019-02-21

申请号：US16113892

申请日：2018-08-27

Applicant: Exact Sciences Development Company, LLC

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US10144953B2

公开(公告)日：2018-12-04

申请号：US15068121

申请日：2016-03-11

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Oliver Hunt ,  Tobias Charles Zutz

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.