公开(公告)号：US20220119862A1

公开(公告)日：2022-04-21

申请号：US17520075

申请日：2021-11-05

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hatim T. Allawi ,  William G. Weisburg ,  Graham P. Lidgard ,  Michael W. Kaiser ,  Abram M. Vaccaro ,  Gracie Shea

IPC: C12Q1/686 ,  C12Q1/6827

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US11118228B2

公开(公告)日：2021-09-14

申请号：US15881409

申请日：2018-01-26

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim T. Allawi ,  Michael W. Kaiser ,  Graham P. Lidgard ,  William R. Taylor ,  Tamara J. Sander ,  Abram M. Vaccaro

IPC: C12Q1/6883 ,  C12Q1/6886 ,  G01N33/533 ,  G16Z99/00 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6853 ,  G01N33/574

Abstract: Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.

公开(公告)号：US11104960B2

公开(公告)日：2021-08-31

申请号：US16570782

申请日：2019-09-13

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： David A. Ahlquist ,  William R. Taylor ,  John B. Kisiel ,  Douglas W. Mahoney ,  Tracy C. Yab ,  Graham P. Lidgard ,  Hatim T. Allawi

IPC: C07H21/04 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6883 ,  G01N33/574

Abstract: Provided herein is technology for esophageal disorder screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of esophageal disorders (e.g., Barrett's esophagus, Barrett's esophageal dysplasia, etc.). In addition, the technology provides methods, compositions and related uses for distinguishing between Barrett's esophagus and Barrett's esophageal dysplasia, and between Barrett's esophageal low-grade dysplasia, Barrett's esophageal high-grade dysplasia, and esophageal adenocarcinoma within samples obtained through endoscopic brushing or nonendoscopic whole esophageal brushing or swabbing using a tethered device (e.g. such as a capsule sponge, balloon, or other device).

公开(公告)号：US20210172023A1

公开(公告)日：2021-06-10

申请号：US17110985

申请日：2020-12-03

Applicant: Exact Sciences Development Company, LLC

Inventor： Ilse A. Tyler ,  Keith Yaeger ,  Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12N15/11 ,  C12Q1/6876

Abstract: The present invention provides synthetic DNA strands that find use as controls or in nucleic acid testing methods. In particular, provided herein are synthetic DNA strands of known composition for use as control molecules in stool DNA testing, e.g., of mutations and/or methylation of DNA isolated from stool samples.

公开(公告)号：US20210155993A1

公开(公告)日：2021-05-27

申请号：US17078924

申请日：2020-10-23

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Chateen Krueger ,  Michael W. Kaiser ,  Tamara J. Sander

IPC: C12Q1/6886

Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

公开(公告)号：US20210079451A1

公开(公告)日：2021-03-18

申请号：US17031383

申请日：2020-09-24

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Tamara J. Sander ,  Maria Giakoumopoulos ,  Michael W. Kaiser ,  Melissa M. Gray ,  Abram Michael Vaccaro

IPC: C12Q1/6806 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6837

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US10808286B2

公开(公告)日：2020-10-20

申请号：US15971461

申请日：2018-05-04

Applicant: Exact Sciences Development Company, LLC

Inventor： Manon Van Engeland ,  Manon Adriaan De Bruine ,  Arjan Griffioen ,  Joost Louwagie ,  Katja Bierau ,  Gontran Brichard ,  Gaetan Otto ,  Maarten Penning

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6886

Abstract: A method of detecting a predisposition to, or the incidence of, cancer in a sample comprises detecting an epigenetic change in at least one gene selected from an NDRG4/NDRG2 subfamily gene, GATA4, OSMR, GATA5, SFRP1, ADAM23, JPH3, SFRP2, APC, MGMT, TFPI2, BNIP3, FOXE1, SYNE1, SOX17, PHACTR3 and JAM3, wherein detection of the epigenetic change is indicative of a predisposition to, or the incidence of, cancer. Also described are pharmacogenetic methods for determining suitable treatment regimens for cancer and methods for treating cancer patients, based around selection of the patients according to the methods of the invention. The present invention is also concerned with improved methods of collecting, processing and analyzing samples, in particular body fluid samples. These methods may be useful in diagnosing, staging or otherwise characterizing various diseases. The invention also relates to methods for identifying, diagnosing, staging or otherwise characterizing cancers, in particular gastrointestinal cancers such as colorectal cancers, gastric cancers and oesophageal cancers. The methods of the invention relate, inter alia, to isolating and analyzing the human DNA component from faecal samples and blood-based samples.

公开(公告)号：US20200248245A1

公开(公告)日：2020-08-06

申请号：US16789276

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/6818 ,  C12Q1/686

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US10683555B2

公开(公告)日：2020-06-16

申请号：US15978565

申请日：2018-05-14

Applicant: Mayo Foundation for Medical Education and Research ,  EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： David A. Ahlquist ,  John B. Kisiel ,  William R. Taylor ,  Tracy C. Yab ,  Douglas W. Mahoney ,  Graham P. Lidgard ,  Hatim T. Allawi

IPC: C07H21/04 ,  C12Q1/68 ,  C12Q1/6886

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as pancreatic and colorectal cancer.

公开(公告)号：US20190218601A1

公开(公告)日：2019-07-18

申请号：US16318580

申请日：2017-07-19

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim T. Allawi ,  Graham P. Lidgard

IPC: C12Q1/6827

CPC classification number: C12Q1/6827 ,  C12Q2523/125 ,  C12Q2531/113 ,  C12Q2545/101 ,  C12Q2561/109 ,  C12Q2600/154

Abstract: Provided herein is technology relating compositions and methods for analysis of methylated DNA from a subject. The technology also relates to use of endogenous methylated DNAs as internal controls for marker gene methylation assays.