公开(公告)号：US20190153510A1

公开(公告)日：2019-05-23

申请号：US16134254

申请日：2018-09-18

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US10253358B2

公开(公告)日：2019-04-09

申请号：US15033803

申请日：2014-11-04

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Michael Domanico ,  Ilse A. Tyler ,  Brian Aizenstein ,  Hatim Allawi ,  Graham P. Lidgard

IPC: C12P19/34 ,  C12Q1/6851 ,  C12Q1/6844

Abstract: The present invention provides DNA plasmids that find use as calibrators or reference standards for calculating DNA quantities in a sample. In particular, provided herein are DNA plasmids that contain multiple control fragments, and methods of their use in DNA quantitation.

公开(公告)号：US10000817B2

公开(公告)日：2018-06-19

申请号：US15165853

申请日：2016-05-26

Applicant: Exact Sciences Development Company, LLC

Inventor： Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.

公开(公告)号：US20210172023A1

公开(公告)日：2021-06-10

申请号：US17110985

申请日：2020-12-03

Applicant: Exact Sciences Development Company, LLC

Inventor： Ilse A. Tyler ,  Keith Yaeger ,  Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12N15/11 ,  C12Q1/6876

Abstract: The present invention provides synthetic DNA strands that find use as controls or in nucleic acid testing methods. In particular, provided herein are synthetic DNA strands of known composition for use as control molecules in stool DNA testing, e.g., of mutations and/or methylation of DNA isolated from stool samples.

公开(公告)号：US20210155993A1

公开(公告)日：2021-05-27

申请号：US17078924

申请日：2020-10-23

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Chateen Krueger ,  Michael W. Kaiser ,  Tamara J. Sander

IPC: C12Q1/6886

Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

公开(公告)号：US20210079451A1

公开(公告)日：2021-03-18

申请号：US17031383

申请日：2020-09-24

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Tamara J. Sander ,  Maria Giakoumopoulos ,  Michael W. Kaiser ,  Melissa M. Gray ,  Abram Michael Vaccaro

IPC: C12Q1/6806 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6837

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US20200248245A1

公开(公告)日：2020-08-06

申请号：US16789276

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/6818 ,  C12Q1/686

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US20190144948A1

公开(公告)日：2019-05-16

申请号：US16168326

申请日：2018-10-23

Applicant: Exact Sciences Development Company, LLC

Inventor： Ilse A. Tyler ,  Keith Yaeger ,  Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6876 ,  C12N15/11

Abstract: The present invention provides synthetic DNA strands that find use as controls or in nucleic acid testing methods. In particular, provided herein are synthetic DNA strands of known composition for use as control molecules in stool DNA testing, e.g., of mutations and/or methylation of DNA isolated from stool samples.

公开(公告)号：US20190048399A1

公开(公告)日：2019-02-14

申请号：US16164373

申请日：2018-10-18

Applicant: Exact Sciences Development Company, LLC

Inventor： Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Oliver Hunt ,  Tobias Charles Zutz

IPC: C12Q1/6806 ,  C12Q1/6851

Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

公开(公告)号：US11499179B2

公开(公告)日：2022-11-15

申请号：US16789276

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6886

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.