公开(公告)号：US3453180A

公开(公告)日：1969-07-01

申请号：US3453180D

申请日：1965-08-02

申请人： MILES LAB

发明人： FRASER JOSEPH WILFRED JR ,  ATKINSON ROGER LEE

IPC分类号： C12Q1/26 ,  C12Q1/54 ,  C12K1/04 ,  G01N33/16

CPC分类号： C12Q1/26 ,  C12Q1/54 ,  Y10S435/805

公开(公告)号：US3438866A

公开(公告)日：1969-04-15

申请号：US3438866D

申请日：1967-03-21

申请人： PFIZER & CO C

发明人： PENICNAK ADRIAN J

IPC分类号： C12Q1/46 ,  C12K1/04

CPC分类号： C12Q1/46 ,  C12Q2334/00

公开(公告)号：US4174772A

公开(公告)日：1979-11-20

申请号：US865278

申请日：1977-12-28

申请人： Norbert Neuss ,  Roger D. Miller

发明人： Norbert Neuss ,  Roger D. Miller

IPC分类号： G01N30/10 ,  G01N30/74 ,  G01N30/80 ,  C12K1/04

CPC分类号： G01N30/74 ,  G01N30/10 ,  G01N30/80

摘要： An apparatus is described which is adaptable for use in coordinating UV/VIS absorbency values of a chromatography eluate containing antimicrobial constituents with bioautograph test results. Such an apparatus is comprised of a UV/VIS spectrophotometer equipped with a continuous recording monitor, a pair of parallel metering valves, a stream splitter, a rotatable drum adapted to hold a piece of absorbent material and means to rotate said drum. The method involves the use of such an apparatus in combination with the bioautograph test to coordinate said absorbency valves with the test results from a plurality of bioautograph test organisms.

摘要翻译： 描述了一种适用于协调含有抗微生物成分的色谱洗脱液的UV / VIS吸收值与Bioautograph测试结果的装置。 这种装置由配备有连续记录监视器，一对平行计量阀，流分离器，适于容纳一块吸收材料的可旋转滚筒和旋转所述滚筒的装置的UV / VIS分光光度计组成。 该方法涉及使用这种装置与生物自动影像测试结合使用来自多个生物自动生物测试生物体的测试结果来协调所述吸光度阀。

公开(公告)号：US4161514A

公开(公告)日：1979-07-17

申请号：US766229

申请日：1977-02-07

申请人： Dee O. Casey

发明人： Dee O. Casey

IPC分类号： C12Q1/02 ,  C12K1/04 ,  G01N1/00 ,  G01N1/28 ,  G01N1/30

CPC分类号： C12Q1/02

摘要： A method is provided for preparing specimens of water containing micro-organisms, for microscopic viewing, employing a composition for immobilizing and also for staining the micro-organisms as an aqueous solution. The composition contains hydrofluoric acid and, advantageously, a small amount of malachite green dye.

摘要翻译： 提供了一种用于制备含微生物水的样品的方法，用于显微镜观察，使用用于固定的组合物以及用于将微生物作为水溶液染色的方法。 组合物含有氢氟酸，有利地是少量的孔雀绿染料。

公开(公告)号：US4137225A

公开(公告)日：1979-01-30

申请号：US888586

申请日：1978-03-20

申请人： Bo T. Af Ekenstam ,  Leif E. Aurell ,  Karl G. Claeson ,  Birgitta G. Karlsson ,  Stig I. Gustavsson ,  Gun A. Olausson

发明人： Bo T. Af Ekenstam ,  Leif E. Aurell ,  Karl G. Claeson ,  Birgitta G. Karlsson ,  Stig I. Gustavsson ,  Gun A. Olausson

IPC分类号： C07K5/083 ,  C07C103/52 ,  C12K1/04

CPC分类号： C07K5/0808 ,  C12Q2337/12 ,  Y10S530/802

摘要： Diagnostically active chromogenic substrate with good stability and high specificity to serine proteases having the formula: H-D-A.sub.1 -A.sub.2 -A.sub.3 -NH-R or salts thereof, wherein A.sub.1 is Gly, Ala, Val, Leu, Ile, Pip, Pro, or Aze; A.sub.2 is Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, or Phe; A.sub.3 is Arg, Lys, or Orn, and R is a chromophoric group.

摘要翻译： 具有下列通式的丝氨酸蛋白酶具有良好的稳定性和高特异性的诊断活性显色底物或其盐，其中A1是Gly，Ala，Val，Leu，Ile，Pip，Pro或 阿泽 A2是Gly，Ala，Val，Leu，Ile，Pip，Pro，Aze或Phe; A3是Arg，Lys或Orn，R是发色基团。

公开(公告)号：US4118575A

公开(公告)日：1978-10-03

申请号：US610671

申请日：1975-09-05

申请人： Meir Rigbi ,  Yakir Klausner ,  Pierre Lefrancier ,  Edgar Sache

发明人： Meir Rigbi ,  Yakir Klausner ,  Pierre Lefrancier ,  Edgar Sache

IPC分类号： G01N31/00 ,  C07C67/00 ,  C07C279/18 ,  C07C301/00 ,  C07C303/40 ,  C07C311/19 ,  C12Q1/37 ,  G01N31/22 ,  C07C149/40 ,  C12K1/04

CPC分类号： C07C2101/14

摘要： The invention relates to novel derivatives of L-phenylalanine and particularly to tosyl-paraguanidino-L-phenylalanine methyl ester, to ter-butyloxycarbonyl derivative of para-guanidino-L-phenylalanine and to para-guanidino-L-phenylalanine.The tosyl-paraguanidino-L-phenylalanine methyl ester is an efficient trypsine substrate.

摘要翻译： 本发明涉及L-苯丙氨酸的新型衍生物，尤其涉及甲磺酰基 - 新亚氨基-L-苯丙氨酸甲酯，对 - 胍基-L-苯丙氨酸的叔丁氧基羰基衍生物和对 - 胍基-L-苯丙氨酸。

公开(公告)号：US4115200A

公开(公告)日：1978-09-19

申请号：US712685

申请日：1976-08-09

申请人： Joseph A. Anderson

发明人： Joseph A. Anderson

IPC分类号： C12M1/32 ,  C12K1/04

CPC分类号： C12M33/04 ,  C12M23/12 ,  C12M23/48 ,  C12M23/50 ,  C12M33/06 ,  C12M37/00

摘要： A motor driven inoculator is provided wherein a plurality of bacteriological transfer loops are mounted on a common head and are activated in response to the positioning of a tray of inoculum to cause the head to be lowered and then raised so that the loops pick up a sample of inoculum. The subsequent positioning of an antibiotic or antimicrobic microtube plate, etc. causes the head to be lowered and raised a second time so that the loops are lowered into the plate to place samples of inoculum in each of a plurality of wells in the plate by dispersion. The removal of the antibiotic plate initiates a sterilizing cycle wherein the head is lowered and raised a third time whereby the loops are heated to a very high temperature by passing electrical current therethrough to volatilize all material thereon and then they are subsequently air cooled prior to the beginning of the next cycle. In an alternative embodiment the inoculum on the loops may be dispersed by percussion.The inoculum tray includes a plurality of grooves and ridges so that a minimal amount of inoculum is necessary for use in placing samples on the loops by dipping.

摘要翻译： 提供一种马达驱动的接种器，其中多个细菌转运回路安装在公共头上并响应于接种物托盘的定位而被激活，以使头降低然后升高，使得回路拾取样品 的接种物。 抗生素或抗微生物微管板等的随后定位使得头部被降低并且第二次升高，使得环被降低到板中，以通过分散体将接种物的样品放置在板中的多个孔中的每个孔中 。 去除抗生素板开始消毒循环，其中头部降低并升高第三次，由此通过使电流通过其将挥发其中的所有材料而将环加热到非常高的温度，然后在它们之前空气冷却 下一个周期的开始。 在替代实施方案中，环上的接种物可以通过敲击分散。

公开(公告)号：US4111752A

公开(公告)日：1978-09-05

申请号：US837365

申请日：1977-09-28

申请人： Howard H. Weetall

发明人： Howard H. Weetall

IPC分类号： C12Q1/12 ,  C12K1/04

CPC分类号： C12Q1/12

摘要： Bacteria of the genus Neisseria can be detected in a sample by separating a lysate of the sample into two parts, incubating one part with a solution of nicotinamide-adenine-dinucleotide (NAD) and 1,2-propanediol, incubating the other part with a solution of only the NAD, and then comparing the products of each incubation for reduction of NAD to NADH. A difference in NADH concentration indicates the presence of any enzyme specific to Neisseria, this enzyme having the capability of oxidizing 1,2-propanediol and reducing NAD. The structure of the enzyme is not completely understood but, because of those two characterizing properties, the nomenclature 1,2-propanediol dehydrogenase therefor is proposed herein.

公开(公告)号：US4104127A

公开(公告)日：1978-08-01

申请号：US698470

申请日：1976-06-18

申请人： Louis Bucalo

发明人： Louis Bucalo

IPC分类号： A61B10/00 ,  A61B10/02 ,  A61F13/22 ,  C12M1/00 ,  C12M1/16 ,  C12K1/04 ,  C12K1/10

CPC分类号： A61B10/02 ,  A61F13/206 ,  C12M21/00 ,  C12M41/34 ,  Y10S435/81

摘要： Cultures of microorganisms can be grown in a body cavity by situating therein a nutrient on a suitable carrier while maintaining the nutrient exposed to a predetermined gaseous atmosphere, so that microorganisms which require a particular atmosphere can grow directly in the body cavity on the nutrient. After a time sufficient for culture growth the carrier with the nutrient thereon are removed from the body cavity and inspected, for example, under a microscope with suitable dyes being used to stain the growth for rendering the latter easily visible. The carrier for the nutrient may take the form of a plastic sheet material having small protuberances which are thicker than a nutrient layer carried by the plastic sheet material and which serve to maintain apart from each other surfaces of the plastic and nutrient which otherwise might engage each other to prevent exposure of the nutrient to the predetermined atmosphere. The article is preferably packaged in a container which has in its interior sufficient moisture to prevent drying of the nutrient, the interior of the container being closed off from the outer atmosphere by the sheet material which is used for the container.

摘要翻译： 微生物的培养物可以通过在体内将营养物置于合适的载体上，同时保持暴露于预定的气体气氛的营养物，从而使需要特定气氛的微生物直接在营养物质的体腔中生长。 在培养生长足够的时间之后，将其上的营养物质的载体从体腔中除去，并且例如在显微镜下检查，使用合适的染料染色生长以使后者容易可见。 用于营养物的载体可以采取具有小的突起的塑料片材的形式，其比由塑料片材承载的营养层更厚，并且其用于保持与塑料和营养物彼此分开的表面，否则其可能与每个 另一种是防止营养物质暴露于预定的气氛。 该制品优选地包装在其内部具有足够的水分以防止营养物干燥的容器中，容器的内部被用于容器的片材从外部大气封闭。

公开(公告)号：US4102748A

公开(公告)日：1978-07-25

申请号：US850040

申请日：1977-11-09

申请人： Mary Frances Vacanti

发明人： Mary Frances Vacanti

IPC分类号： C12M1/26 ,  C12B1/02 ,  C12K1/04

CPC分类号： C12M33/02

摘要： A three plane instrument for plating and streaking a microbiological sample has a hand held stem topped by a conventional first planar loop for forming a film by dipping and at least one partial planar loop co-extensive with the first loop and having one leg spaced from and parallel thereto. After plating, the stem is rotated and the said leg is utilized to streak the plated sample.

摘要翻译： 用于电镀和条纹微生物样品的三平面仪器具有由常规的第一平面回路顶起的手持式茎，用于通过浸渍形成膜，以及至少一个部分平面环与第一环共同延伸，并且具有一条腿与 与其平行。 在电镀之后，杆旋转，并且所述腿用于条纹镀覆的样品。