公开(公告)号：US4675290A

公开(公告)日：1987-06-23

申请号：US713242

申请日：1985-03-18

申请人： Kunio Matsumoto ,  Yoshitaka Kagimoto ,  Tsutomu Hirata ,  Susumu Watanabe ,  Akira Ohtsuka ,  Kiyoharu Takahashi

发明人： Kunio Matsumoto ,  Yoshitaka Kagimoto ,  Tsutomu Hirata ,  Susumu Watanabe ,  Akira Ohtsuka ,  Kiyoharu Takahashi

IPC分类号： C12Q1/26 ,  C12Q1/37 ,  C12Q1/52 ,  C12Q1/36

CPC分类号： C12Q1/52 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q2337/10 ,  Y10S435/817

摘要： A method for assay of enzyme activity which comprises treating an amide compound of the formula ##STR1## with peptidase; treating thus-liberated amine of the formula ##STR2## with an oxidase which consume oxygen and forms pigment by oxidative condensation of said amine with a coupler of the formula ##STR3## and quantitatively measuring the detectable changes. Also disclosed is an assay method which comprises treating an amide compound of the formula ##STR4## or a salt thereof, with peptidase to liberate an aniline derivative of the formula ##STR5## oxidizing said aniline derivative; and quantitatively measuring detectable change.

摘要翻译： 一种测定酶活性的方法，其包括用肽酶处理式“酰基”的酰胺化合物; 用消耗氧气的氧化酶处理如此释放的式“IMAGE”的胺，并通过所述胺与式“IMAGE”的成色剂的氧化缩合形成颜料并定量测量可检测的变化。 还公开了一种测定方法，其包括用肽酶处理式IMA的酰胺化合物或其盐，以释放氧化所述苯胺衍生物的式IMA的苯胺衍生物; 并定量测量可检测的变化。

公开(公告)号：US4503145A

公开(公告)日：1985-03-05

申请号：US352970

申请日：1982-02-26

申请人： Harumi Katsuyama ,  Yoshikazu Amano ,  Asaji Kondo

发明人： Harumi Katsuyama ,  Yoshikazu Amano ,  Asaji Kondo

IPC分类号： G01N33/66 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/48 ,  C12Q1/52 ,  G01N31/22 ,  G01N33/92 ,  G01N33/52

CPC分类号： C12Q1/52 ,  C12Q1/26 ,  Y10S435/805

摘要： An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.

摘要翻译： 用于测定转氨酶活性的分析膜包括含有丙酮酸氧化酶，氢供体和能够形成阳离子染料的成色剂的组合的试剂层和阴离子聚合物。 通过与用于检测过氧化氢的颜色指示剂组合物的反应形成的阳离子染料用阴离子聚合物固定，从而阻止阳离子染料的移动和扩散，使得分析膜实现高检测灵敏度和高精度。

公开(公告)号：US4329425A

公开(公告)日：1982-05-11

申请号：US168286

申请日：1980-07-10

申请人： Giorgio Ricci ,  Giorgio Federici

发明人： Giorgio Ricci ,  Giorgio Federici

IPC分类号： G01N33/50 ,  C12Q1/52

CPC分类号： C12Q1/52 ,  Y10S435/81

摘要： A kinetic UV method of determining GOT and GPT by reacting transaminase with a substrate uses L-cysteinsulfinic acid and L-alanine as the substrate and lactic dehydrogenase as the revealing enzyme.

摘要翻译： 通过使转氨酶与底物反应来测定GOT和GPT的动力学UV方法使用L-半胱氨酸亚磷酸和L-丙氨酸作为底物，以及乳酸脱氢酶作为揭示酶。

公开(公告)号：US4271265A

公开(公告)日：1981-06-02

申请号：US59368

申请日：1979-07-20

申请人： Ulfert Deneke ,  Peter Stahl ,  Walter Schneider

发明人： Ulfert Deneke ,  Peter Stahl ,  Walter Schneider

IPC分类号： C12Q1/32 ,  C12Q1/52 ,  G01N33/48

CPC分类号： C12Q1/52 ,  Y10S435/805

摘要： In a method for the determination of glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate, with formation of .alpha.-ketoglutarate in a buffered solution, the .alpha.-ketoglutarate formed is reacted with .gamma.-aminobutyrate in the presence of .gamma.-aminobutyrate transaminase with formation of succinate semialdehyde, NADP is reduced with the latter in the presence of succinate semialdehyde dehydrogenase to give NADPH, and the latter is measured either directly or after conversion with a tetrazolium salt and an electron carrier such as diaphorase, phenantroline methosulphate or phenazine methosulphate to a formazane dye.A reagent suited for use with this method contains .gamma.-aminobutyrate transaminase, succinate semialdehyde dehydrogenase, .gamma.-aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate, and optionally a tetrazolium salt, an electron carrier and a surface-active agent.

摘要翻译： 在通过草酸乙酯或丙酮酸与谷氨酸的反应测定谷氨酸 - 草乙酸转氨酶或谷氨酸 - 丙酮酸转氨酶的方法中，在缓冲溶液中形成α-酮戊二酸，所形成的α-酮戊二酸与γ-氨基丁酸酯在 存在具有琥珀酸盐半醛形成的γ-氨基丁酸转氨酶，NADP在琥珀酸半醛脱氢酶存在下还原，得到NADPH，后者直接或用四唑鎓盐和电子载体如心肌黄酶转化后测量 ，异丙基硫酸甲酯或吩嗪硫酸甲酯加成甲ane染料。 适用于该方法的试剂含有γ-氨基丁酸转氨酶，琥珀酸半醛脱氢酶，γ-氨基丁酸盐，谷氨酸盐和缓冲液，以及草酸乙酯或丙酮酸盐，以及任选的四唑鎓盐，电子载体和表面活性剂。

公开(公告)号：US4024021A

公开(公告)日：1977-05-17

申请号：US575879

申请日：1975-05-09

申请人： William S. Stavropoulos ,  Kenneth J. Acuff

发明人： William S. Stavropoulos ,  Kenneth J. Acuff

IPC分类号： C12Q1/52 ,  G01N31/14

CPC分类号： C12Q1/52

摘要： Glutamate and glutamic transaminases are determined by mixing a substrate-reagent composition that is essentially free of ammonia or ammonium ions with a specimen to be analyzed, incubating the resulting mixture for less than about 15 minutes, terminating incubation and measuring a color produced.

摘要翻译： 谷氨酸和谷氨酸转氨酶通过将基本上不含氨或铵离子的底物试剂组合物与待分析的样品混合，将所得混合物孵育少于约15分钟，终止培养并测量所产生的颜色来测定。

公开(公告)号：US4017365A

公开(公告)日：1977-04-12

申请号：US512620

申请日：1974-10-07

申请人： Toshimasa Nakayama ,  Motoshi Kitamura

发明人： Toshimasa Nakayama ,  Motoshi Kitamura

IPC分类号： C12Q1/48 ,  C12Q1/32 ,  C12Q1/52 ,  G01N31/14

CPC分类号： C12Q1/52 ,  C12Q1/32

摘要： Difficulty in using the Karmen method due to lipemic turbidity and substances absorbing ultraviolet rays in serum can be prevented by reducing the amount of GOT or GPT-containing sample and adding nonion surfactant to the reaction system.

摘要翻译： 通过减少含GOT或含GPT的样品并向反应体系中加入非离子表面活性剂，可以防止由于血液浊度而使用Karmen方法难以吸收血清中的紫外线。

公开(公告)号：US3950133A

公开(公告)日：1976-04-13

申请号：US467550

申请日：1974-05-06

申请人： Alexander A. Monte ,  Ching Chiang

发明人： Alexander A. Monte ,  Ching Chiang

IPC分类号： C12Q1/30 ,  C12Q1/32 ,  C12Q1/42 ,  C12Q1/52 ,  C12Q1/54 ,  C12Q1/58 ,  C12Q1/62 ,  G01N33/50 ,  G01N33/68 ,  G01N33/72 ,  G01N33/84 ,  G01N33/16

CPC分类号： C12Q1/42 ,  C12Q1/30 ,  C12Q1/32 ,  C12Q1/52 ,  C12Q1/54 ,  C12Q1/58 ,  C12Q1/62 ,  G01N33/50 ,  G01N33/68 ,  G01N33/721 ,  G01N33/84 ,  Y10T436/166666

摘要： A solid, water-soluble substantially anhydrous, storage-stable reagent formulation for use in conducting a clinical diagnostic test on a biological specimen is provided. The reagent formulation comprises a mixture containing a reagent capable of participating in a test reaction to effect a measurable change in a test system; and a nitrogen bearing polyoxyalkylene nonionic surfactant. The surfactant has a structure corresponding to that obtained when ethylene diamine is reacted sequentially with propylene oxide and ethylene oxide in the presence of a catalyst. The polyoxypropylene chains of said surfactant have an average molecular weight of between about 750 and about 6750. Methods of preparing the reagent formulations and methods of using them to conduct clinical diagnostic tests are also provided.

公开(公告)号：US3814669A

公开(公告)日：1974-06-04

申请号：US29534872

申请日：1972-10-05

申请人： DOW CHEMICAL CO

发明人： GOLDENBERG H

IPC分类号： C12Q1/52 ,  G01N31/14

CPC分类号： C12Q1/52

摘要： AN IMPROVED METHOD OF DETERMINING AMOUNTS OF THE ENZYME GULTAMIC-OXALACETIC TRANSMINASE IN BODY FLUIDS IS DECRIBED. THE METHOD IS BASED ON THE REACTION BETWEEN A DIAZONIUM SALT COLOR DEVELOPER AND OXALACETIC ACID PRODUCED DURING INCUBATION OF THE BODY FLUID WITH A SUITABLE SUBSTRATE AND THE COMPARISON OF THE RECIPROCAL OF THE DEPTH OF COLOR OBTANED THEREBY TO THE RECIPROCAL OF THE DEPTH OG COLOR OBTANED WITH A KNOWN AMOUNT OF SAID ENZYLE.

公开(公告)号：US3684451A

公开(公告)日：1972-08-15

申请号：US3684451D

申请日：1969-08-20

申请人： MILES LAB

发明人： LINOLI GIANNI ,  MANNUCCI ENZO SERGIO

IPC分类号： C08F8/30 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/52 ,  G01N33/52 ,  C07G7/02 ,  C08F27/08 ,  G01N31/00

CPC分类号： C12Q1/52 ,  C08F8/30 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q2334/30 ,  C12Q2334/70 ,  G01N33/52 ,  Y10S435/805 ,  Y10S435/814 ,  Y10T436/173845 ,  Y10T436/203332

摘要： A NOVEL INSOLUBLE POLYMERIC DIAZONIUM SALT COLOR DEVELOPER CONSISTING OF A DIAZOTIZED AROMATIC POLYAMINE BOUND BY AN AMIDE LINKAGE TO A CATION EXCHANGE RESIN CAN BE PREPARED BY REACTING A CATION EXCHANGE RESIN CONTAINING ACID GROUPS WITH A HALOGENATING AGENT TO CONVERT SOME OF THE RESIN ACID GROUPS TO ACYL HALIDE GROUPS, REACTING THE RESULTING ACYL HALIDE GROUPS WITH AN AROMATIC POLYAMINE TO FORM A RESIN CONTAINING AMIDE LINKAGES AND FREE AROMATIC AMINO GROUPS, AND THEN DIAZOTIZING THE FREE AROMATIC AMINO GROUPS. THE RESULTING INSOLUBLE COLOR DEVELOPER CAN BE USED TO DETECT COUPLING COMPOUNDS BY FORMING COLORED REACTION PRODUCTS WITH SUCH COUPLING COMPOUNDS. THE COLOR DEVELOPER CAN ALSO BE USED TO DETECT AN ENZYME SYSTEM WHEREIN THE ENZYME CATALYZES A REACTION WITH A SUITABLE SUBSTRATE TO RELEASE A COUPLING COMPOUND WHICH WILL FORM A COLORED REACTION PRODUCT WITH THE INSOLUBLE COLOR DEVELOPER.

公开(公告)号：US3458403A

公开(公告)日：1969-07-29

申请号：US3458403D

申请日：1966-05-05

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： KATSUNUMA NOBUHIKO

IPC分类号： C12Q1/52 ,  C12K1/06

CPC分类号： C12Q1/52