公开(公告)号：US20210394083A1

公开(公告)日：2021-12-23

申请号：US17464107

申请日：2021-09-01

Applicant: Cytiva Sweden AB

Inventor： Per Karlberg ,  Erik Rurling ,  Joakim Lundkvist

IPC: B01D15/20 ,  G01N30/56

Abstract: A method for packing a media bed in a column (3). According to the invention the method comprises the steps of: providing data to a control unit (15) connected to the column (3), said data comprising at least a measured slurry concentration, a target bed height, a target packing factor or compression factor and minimum and maximum acceptable values for at least one of target bed height and target packing or compression factor; forcing a movable adapter (9) along a longitudinal axis of the column (3) in order to pack the media; detecting when the media bed is consolidated; the control unit (15) processing the provided data and the information about the consolidated bed height in order to present to the user acceptable bed heights, if there are any, giving acceptable packing or compression factors.

公开(公告)号：US11110398B2

公开(公告)日：2021-09-07

申请号：US15750218

申请日：2016-08-17

Applicant: Cytiva Sweden AB

Inventor： Sasi Kumar Nutalapati ,  Klaus Gebauer ,  Karl Axel Jakob Liderfelt ,  Nachiket Karmarkar ,  Ajit S. Vernekar ,  Amit Kumar Sharma ,  Fredrik Oskar Lundstrom ,  Sangeetha Raghu Ramachandran

IPC: C07K1/34 ,  B01D61/18 ,  B01D61/20 ,  B01D61/22 ,  B01D61/08 ,  B01D61/10 ,  B01D61/12 ,  B01D65/02

Abstract: The disclosure generally relates to methods and apparatus for the efficient quantitative recovery of valuable biological fluids from filtration systems, more particularly to efficient quantitative recovery of valuable biological fluids from high precision separation systems suitable for use in the pharmaceutical and biotechnology industries.

公开(公告)号：US20210263482A1

公开(公告)日：2021-08-26

申请号：US17268490

申请日：2019-08-30

Applicant: Cytiva Sweden AB

Inventor： Mikael Johansson ,  Pasi Juntikka ,  Peter Toreheim ,  Shivakumar Selvaraj

IPC: G05B13/02

Abstract: The present invention relates to a computer implemented method (600) for optimization of a bioprocessing system (100) formed by interconnected or interconnectable bioprocessing units and configured to provide a desired system functionality, the method comprising obtaining (610) requirement data of a bioprocess, the requirement data being at least indicative of the desired system functionality, and indicative of a desired substance resulting from the operation of the bioprocess system, obtaining (620) unit data indicative of characteristics of a plurality of bioprocessing units, generating (630) at least one optimized or suitable bioprocessing system configuration capable to provide the desired system functionality, the bioprocessing system configuration comprising a selection of bioprocessing units from the plurality of bioprocessing units selected using the requirement data, the unit data and an objective function dependent on the requirement data and the unit data. The invention extends to a configuration system (500).

公开(公告)号：US20210223079A1

公开(公告)日：2021-07-22

申请号：US16768956

申请日：2018-12-04

Applicant: Cytiva Sweden AB

Inventor： Philipp Lang ,  Jens Ruetten ,  Charles E. Seeley

IPC: G01F1/84 ,  G01F15/02

Abstract: Provided is a Coriolis flow sensor assembly that includes a flow tube configured to provide a flow path through the flow tube. Further, the Coriolis flow sensor assembly includes a mechanical drive assembly configured to drive an oscillation of the flow tube while fluid is flowing via an oscillation surface. The Coriolis flow sensor assembly includes an interface fixedly coupled to the oscillation surface of the mechanical drive assembly and configured to receive the flow tube.

公开(公告)号：US11047859B2

公开(公告)日：2021-06-29

申请号：US15317619

申请日：2015-06-19

Applicant: CYTIVA SWEDEN AB

Inventor： Olof Karlsson

IPC: G01N21/552 ,  G01N33/68 ,  G01N21/27

Abstract: The invention relates to a method for normalization of a label-free system for calibration-free concentration analysis. The method comprises (1) providing a solution containing a control macromolecular particle of a known concentration at a pH lower than the pI of the macromolecular particle and a low ionic strength; (2) contacting the solution with a negatively charged optical sensor surface at a first flow rate to allow electrostatic binding of the macromolecular particle to the surface and obtaining a first sensorgram; (3) contacting the solution with the optical sensor surface at a second flow rate to allow electrostatic binding of the macromolecular particle to the surface and obtaining a second sensorgram; and (4) fitting the sensorgrams to a binding equation to determine a measured concentration of the control; wherein the optical sensor surface is not immobilized with a ligand for the control and the contacting steps are performed under mass transport limitations. Also provided is a kit for performing the method, as well as a method for determining a concentration of an analyte.

公开(公告)号：US10989709B2

公开(公告)日：2021-04-27

申请号：US15465970

申请日：2017-03-22

Applicant: CYTIVA SWEDEN AB

Inventor： Radislav Alexandrovich Potyrailo ,  Andrew David Pris ,  Nandini Nagraj ,  Anthony John Murray

IPC: G01N33/53 ,  G01N33/543

Abstract: A method of recovering a target from a sample is provided. The method of recovering the target follows different steps. The steps include providing a binding element, wherein the binding elements are immobilized on a solid support, adding the sample comprising the target to the binding element to form a binding element-target complex; washing the binding element-target complex; and eluting the target from the binding element-target complex. The system for reversible detection of target in a range from 2 to 1,000,000 bind/release cycles is also provided.

公开(公告)号：US10981947B2

公开(公告)日：2021-04-20

申请号：US15122609

申请日：2015-03-12

Applicant: Cytiva Sweden AB

Inventor： Bjorn Markus Olovsson ,  Mathias Osmark ,  Mangus Gamberg ,  Daniel Carlsson

IPC: C07K1/04 ,  B65B31/04 ,  B01J19/00 ,  C07K1/06 ,  C07K1/08

Abstract: The invention relates to a container holder 10, comprising a main body 12 which in turn comprises a gas inlet 16; a solution liquid outlet 18; a gas control valve 20 through which a gas enters the container 100 from the gas inlet; and a sealing means 22 for the container, which sealing means includes a passageway 24 for the input of gas and output of a solution in the container via an egress tube 19; wherein when the container is connected to the container holder through the sealing means, the gas control valve opens automatically, and when the container is disconnected the gas control valve is closed automatically. The invention further relates to a container panel 50 which includes two or more container holders. Also disclosed are methods of using these containers and container panels for synthesizing a polypeptide.

公开(公告)号：US10816459B2

公开(公告)日：2020-10-27

申请号：US16130100

申请日：2018-09-13

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring

IPC: G01N21/33 ,  G01N21/05 ,  G01N21/59 ,  A61L2/08 ,  G01N30/74 ,  G01N21/03 ,  G01N33/487

Abstract: The present invention relates to a flow cell (10) comprising a fluid inlet (16) and a fluid outlet (18) separated by a sample flow-through chamber (12) comprising at least one UV-transparent window (22′), wherein the at least one UV-transparent window (22′) is made of a polymer material and has been subjected to Gamma radiation sterilisation. In one aspect, the flow cell is combustible.

公开(公告)号：US12297418B2

公开(公告)日：2025-05-13

申请号：US17235195

申请日：2021-04-20

Applicant: Cytiva Sweden AB

Inventor： Christian Kaisermayer

IPC: C12M1/34 ,  C12M1/00 ,  C12M1/12 ,  C12M3/00

Abstract: The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be aseptically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

公开(公告)号：US12247958B2

公开(公告)日：2025-03-11

申请号：US17772902

申请日：2020-11-17

Applicant: Cytiva Sweden AB

Inventor： Nils Stafstrom

IPC: G01N30/32 ,  G01N30/86 ,  G01N30/02

Abstract: Disclosed is a method for determining an operating flow rate for a chromatographic column (4) in an HPLC system (1). The method comprises: measuring/calculating a pressure of the system (1) without the chromatographic column (4) for one or more flow rates; fitting a function to the flow rate(s) and corresponding pressure(s), calculating from the function and a predetermined recommended flow rate for the chromatographic column (4) a system pressure drop at the predetermined recommended flow rate. An operating flow rate is determined by summing the system pressure drop and a maximal column pressure limit, and determining a contribution of the system pressure drop to the summed pressure. If this contribution exceeds 1% an operating flow rate for the column is determined to a flow rate that corresponds to a pressure at a pressure monitor arranged before the column that is lower than the predetermined maximum column pressure limit.