公开(公告)号：US20220137039A1

公开(公告)日：2022-05-05

申请号：US17577138

申请日：2022-01-17

Applicant: CYTIVA SWEDEN AB

Inventor： Olof Karlsson

IPC: G01N33/543 ,  G01N33/557

Abstract: The present invention relates to a method for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor, comprising the steps of providing (101) a plurality of fluid samples, each containing known concentrations of analyte providing (102) a plurality of needles and a plurality of sensor surfaces or detection spots, at least some of the sensor surfaces or detection spots having a known amount of ligand immobilized thereon, and each needle being configured to inject a fluid sample to a sensor surface or detection spots dividing (103) said plurality of fluid samples into at least two groups injecting (104) the fluid samples of a first of said groups to the sensor surfaces or detection spots by means of the needles to permit association of the analyte to the ligand monitoring (104) each sensor surface or detection spot and collecting binding data, and repeating (105) the steps of injecting fluid samples and monitoring detection spots and collecting binding data for each group of fluid samples, wherein the steps above are performed sequentially, without intermediate regeneration or renewal of the immobilized ligand. The invention also relates to a biosensor system for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface, to software for performing the steps of the method and to a computer readable medium for storing said software.

公开(公告)号：US11796536B2

公开(公告)日：2023-10-24

申请号：US16932453

申请日：2020-07-17

Applicant: CYTIVA SWEDEN AB

Inventor： Olof Karlsson

IPC: G01N33/543

CPC classification number: G01N33/54373 ,  G01N33/54393

Abstract: The present invention provides a method of assaying a sample solution for the presence of a first analyte comprising: (a) providing a sensor surface having a ligand immobilized thereto; (b) flowing the sample solution over the sensor surface; and (c) detecting the presence or absence of binding of the analyte to the ligand on the sensor surface;
wherein the contact time between the sample solution and the immobilized ligand is less than 15 seconds.

公开(公告)号：US20220336042A1

公开(公告)日：2022-10-20

申请号：US17640771

申请日：2020-09-29

Applicant: Cytiva Sweden AB

Inventor： Tobias Soderman ,  Olof Karlsson ,  Magnus Raberg ,  Andreas Borgstrom

IPC: G16B15/00 ,  G16B40/20 ,  G01N21/552 ,  G01N21/27 ,  G06N3/08

Abstract: Disclosed is a method for classifying monitoring results from an analytical sensor system (20) arranged to monitor molecular interactions at a sensing surface, wherein detection curves representing progress of the molecular interactions with time are produced. The method comprises steps of: acquiring (100) a set of detection curves, fitting (101) a first mathemati- cal model to the set of detection curves; calculating (102) a set of features from the set of detection curves and fitted mathematical model; based on the calculated set of features, classifying (103) each detection curve into qual- ity classification group; and based on the classification determining which detection curves to use in kinetic analysis of the monitored molecular inter- actions.

公开(公告)号：US20220336041A1

公开(公告)日：2022-10-20

申请号：US17636288

申请日：2020-09-08

Applicant: Cytiva Sweden AB

Inventor： Fredrik Edebratt ,  Olof Karlsson ,  Anna Lager ,  Jens Larsson

IPC: G16B15/00 ,  G01N33/68 ,  G16B40/20 ,  G16B45/00

Abstract: Disclosed is a method of qualifying a subgroup of target binding biomolecules from a larger group of target binding biomolecules for analysis. A competitive immunoassay including a target protein is used to identify 100 interactions between different pairs of the target binding biomolecules and interaction profiles are generated 200. Each target binding biomolecule is allocated 300 to a bin representing an epitope family and identified bins are associated in a circular or semi-circular bin chart on a display with identified respective target binding biomolecule(s). Based on the association 400 between identified bins and identified respective target binding molecule(s) in the bin chart, a subgroup of target binding biomolecules is selected 500 for further analysis by selecting one or more of the target binding biomolecule(s) of one or more of the bins.

公开(公告)号：US11255852B2

公开(公告)日：2022-02-22

申请号：US15519238

申请日：2015-10-26

Applicant: CYTIVA SWEDEN AB

Inventor： Olof Karlsson ,  Henrik Berling

IPC: G01N33/50 ,  G01N33/543 ,  G01N33/53 ,  G01N33/483 ,  G01N21/552 ,  G01N21/64 ,  B01L3/00 ,  B01F13/00

Abstract: In one embodiment, the present invention relates to a method for establishing a solvent correction curve as well as using the curve for obtaining a corrected sensorgram or corrected report points from a sensorgram of an analyte. In another embodiment, the present invention provides an analytical system for studying molecular interactions, which comprises computer processing means including program code means for performing the steps of the methods. Also provided is a computer program product comprising program code means stored on a computer readable medium or carried on an electrical or optical signal for performing the steps of the methods.

公开(公告)号：US20230228676A1

公开(公告)日：2023-07-20

申请号：US17998236

申请日：2021-06-22

Applicant: CYTIVA SWEDEN AB

Inventor： Tobias Soderman ,  Olof Karlsson ,  Paul E. Belcher

IPC: G01N21/27 ,  G16C20/70 ,  G16C20/50

CPC classification number: G01N21/272 ,  G16C20/70 ,  G16C20/50

Abstract: Disclosed is a method for classifying monitoring results from an analytical sensor system (20), by allowing (100) a first set of analyte sample solutions to interact with a ligand (3) and acquiring (101) a set of response data, extracting (102) at least one interaction parameter from the response data, and for each analyte sample solution providing (103) a trained machine learning algorithm with the interaction parameter(s). The trained machine learning algorithm classifies (104) each analyte sample solution based on the interaction parameter(s) into at least one quality classification group indicative of the interaction of the analyte sample solution with the ligand (3). The machine learning algorithm is trained (200) using a set of interaction parameters extracted from response data obtained from interactions between a second set of analyte sample solutions with at least one ligand (3), and at least one quality classification group indicative of the interaction of the analyte sample solution with the ligand (3).

公开(公告)号：US11255851B2

公开(公告)日：2022-02-22

申请号：US15760754

申请日：2016-09-22

Applicant: CYTIVA SWEDEN AB

Inventor： Olof Karlsson

IPC: G01N33/557 ,  G01N33/543 ,  G01N21/41 ,  G01N21/552

Abstract: A system and method for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor is provided. In one example, the system includes a plurality of needles, each being arranged to inject a fluid sample to one of sensor surfaces or detection spots. A plurality of fluid samples, each containing known concentrations of analyte, is provided. The plurality of fluid samples may be divided into at least two groups, each group having a number of fluid samples corresponding to the number of needles. The system and method is configured to perform the injections without intermediate regeneration or renewal of the immobilized ligand. Software for performing the steps of the method and a computer readable medium for storing the software are also provided.

公开(公告)号：US11047859B2

公开(公告)日：2021-06-29

申请号：US15317619

申请日：2015-06-19

Applicant: CYTIVA SWEDEN AB

Inventor： Olof Karlsson

IPC: G01N21/552 ,  G01N33/68 ,  G01N21/27

Abstract: The invention relates to a method for normalization of a label-free system for calibration-free concentration analysis. The method comprises (1) providing a solution containing a control macromolecular particle of a known concentration at a pH lower than the pI of the macromolecular particle and a low ionic strength; (2) contacting the solution with a negatively charged optical sensor surface at a first flow rate to allow electrostatic binding of the macromolecular particle to the surface and obtaining a first sensorgram; (3) contacting the solution with the optical sensor surface at a second flow rate to allow electrostatic binding of the macromolecular particle to the surface and obtaining a second sensorgram; and (4) fitting the sensorgrams to a binding equation to determine a measured concentration of the control; wherein the optical sensor surface is not immobilized with a ligand for the control and the contacting steps are performed under mass transport limitations. Also provided is a kit for performing the method, as well as a method for determining a concentration of an analyte.