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    Macroporous polymeric carrier for covalently binding proteins, its preparation and its use for fixing active proteins
    124.
    发明授权
    Macroporous polymeric carrier for covalently binding proteins, its preparation and its use for fixing active proteins 失效
    用于共价结合蛋白质的大孔聚合物载体，其制备及其用于固定活性蛋白质的用途

    公开(公告)号：US4266030A

    公开(公告)日：1981-05-05

    申请号：US146400

    申请日：1980-05-05

    申请人： Chung-Ji Tschang ,  Heinrich Klefenz ,  Axel Sanner

    发明人： Chung-Ji Tschang ,  Heinrich Klefenz ,  Axel Sanner

    IPC分类号： C08F8/00 ,  C08F8/18 ,  C08F8/28 ,  C08F8/30 ,  C08F8/32 ,  C08F8/34 ,  C08H1/00 ,  C12N11/08 ,  C08J9/00

    CPC分类号： C12N11/08 ,  C08F8/00

    摘要： A macroporous crosslinked styrene resin, used as a carrier for covalently binding proteins, which resin contains isocyanate, thioisocyanate or aldehyde groups as protein-binding groups and may or may not contain sulfonic acid groups--which may also be in the form of the sodium salt or of sulfonic acid amide groups--as hydrophilic groups.The carrier according to the invention is prepared from a sulfochlorinated macroporous crosslinked styrene resin by reacting the sulfonic acid chloride groups with an .alpha.,.omega.-diamino compound, with hydrazine or with an .alpha., .omega.-diamino-diether, converting any sulfonic acid chloride groups which may still be present to free sulfonic acid groups, their sodium salt or a sulfonamide group, and then reacting the terminal amino groups with phosgene, thiophosgene or a diisocyanate in order to produce the binding groups, which in turn fix a biologically active protein by covalent bonds.

    摘要翻译： 用作共价结合蛋白质的载体的大孔交联苯乙烯树脂，该树脂含有异氰酸酯，硫代异氰酸酯或醛基作为蛋白质结合基团，并且可以含有也可以不含磺酸基团，其也可以是钠盐形式 或磺酰胺基 - 作为亲水基团。 根据本发明的载体由磺基氯化大孔交联苯乙烯树脂通过使磺酰氯基团与α，ω-二氨基化合物与肼或与α，ω-二氨基二醚反应而制备，转化任何磺酰氯基团 其仍然可以存在以游离磺酸基团，它们的钠盐或磺酰胺基团，然后使末端氨基与光气，硫光气或二异氰酸酯反应，以产生结合基团，其又通过以下方式固定生物活性蛋白质 共价键。