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    Methods of reducing odor
    132.
    发明授权
    Methods of reducing odor 有权

    公开(公告)号：US10954477B2

    公开(公告)日：2021-03-23

    申请号：US15742076

    申请日：2016-07-06

    Applicant: NOVOZYMES A/S

    Inventor： Jesper Vind ,  Lone Baunsgaard ,  Garry Paul Gippert

    IPC: C12N9/20 ,  C11D3/386 ,  C12N15/52

    Abstract: The present invention relates to reduced odor. More specifically the invention relates to a method for hydrolyzing a lipase substrate comprising: adding to said substrate a lipase variant of SEQ ID NO: 3, which variant comprises a substitution at one or more positions corresponding to positions 4; 44; 45; 49; 58; 84; 88; 93; 95; 97; 104; 108; 131; 140; 147; 152; 158; 165; 176; 177; 187; 195; 199; 208; 211; 221; 222; 224; 232; 244; 247; 248; 249; 251; 255; and 259 of SEQ ID NO: 3, has lipase activity, and has at least 75% but less than 100% sequence identity to SEQ ID NO: 3 or a fragment thereof with lipase activity, in which method odor is reduced when compared to the method wherein SEQ ID NO: 3 is added to the lipase substrate. The present invention also relates lipase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.

    METHODS OF REDUCING ODOR
    134.
    发明申请
    METHODS OF REDUCING ODOR 有权

    公开(公告)号：US20210079318A1

    公开(公告)日：2021-03-18

    申请号：US17075185

    申请日：2020-10-20

    Applicant: Novozymes A/S

    Inventor： Jesper Vind ,  Lone Baunsgaard ,  Garry Paul Gippert

    IPC: C11D3/386 ,  C12N9/20 ,  C12N15/52

    Abstract: The present invention relates to reduced odor. More specifically the invention relates to a method for hydrolyzing a lipase substrate comprising: adding to said substrate a lipase variant of SEQ ID NO: 3, which variant comprises a substitution at one or more positions corresponding to positions 4; 44; 45; 49; 58; 84; 88; 93; 95; 97; 104; 108; 131; 140; 147; 152; 158; 165; 176; 177; 187; 195; 199; 208; 211; 221; 222; 224; 232; 244; 247; 248; 249; 251; 255; and 259 of SEQ ID NO: 3, has lipase activity, and has at least 75% but less than 100% sequence identity to SEQ ID NO: 3 or a fragment thereof with lipase activity, in which method odor is reduced when compared to the method wherein SEQ ID NO: 3 is added to the lipase substrate. The present invention also relates lipase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.

    Methods of reducing odor
    137.
    发明授权
    Methods of reducing odor 有权

    公开(公告)号：US10920203B2

    公开(公告)日：2021-02-16

    申请号：US15580853

    申请日：2016-07-01

    Applicant: NOVOZYMES A/S

    Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

    IPC: C12N9/20 ,  C11D3/386

    Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

    Fungal Chaperone Proteins
    140.
    发明申请
    Fungal Chaperone Proteins 有权

    公开(公告)号：US20210010010A1

    公开(公告)日：2021-01-14

    申请号：US17040906

    申请日：2019-03-25

    Applicant: Novozymes A/S

    Inventor： Jean-Marie Mouillon ,  Pernille Hvid Christensen

    IPC: C12N15/80

    Abstract: The present invention relates to fungal host cells comprising nucleic acid constructs comprising a heterologous promoter operably linked to polynucleotidea encoding a chaperone, nucleic acid constructs comprising a polynucleotide encoding a chaperone, and methods for producing polypeptides of interest.

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