公开(公告)号：US20240060060A1

公开(公告)日：2024-02-22

申请号：US18484214

申请日：2023-10-10

Applicant: Novozymes A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386 ,  C12N1/20 ,  C12N15/52 ,  C12N1/16 ,  C12N1/14

CPC classification number: C12N9/20 ,  C11D3/38627 ,  C11D3/38681 ,  C12N1/20 ,  C12N15/52 ,  C12N1/16 ,  C12N1/14

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/SN, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/LN/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US20230363419A1

公开(公告)日：2023-11-16

申请号：US18029586

申请日：2021-10-07

Applicant: Novozymes A/S

Inventor： Steffen Kayser ,  Christian Nyffenegger ,  Marianne Thorup Cohn ,  Flemming Borup

IPC: A23K20/189 ,  A23K30/00

CPC classification number: A23K20/189 ,  A23K30/00 ,  C12Y111/01016 ,  C12Y111/01021 ,  C12Y115/01001

Abstract: Animal feeds are preserved using an enzymatic antioxidant. Polypeptides having catalase activity and/or superoxide dismutase activity preserve animal feed or animal feed additives or prevent the degradation of the proteins, vitamins fats and lipids contained in animal feed components.

公开(公告)号：US20210108184A1

公开(公告)日：2021-04-15

申请号：US17128344

申请日：2020-12-21

Applicant: Novozymes A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US10920203B2

公开(公告)日：2021-02-16

申请号：US15580853

申请日：2016-07-01

Applicant: NOVOZYMES A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US11814656B2

公开(公告)日：2023-11-14

申请号：US17816892

申请日：2022-08-02

Applicant: Novozymes A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386 ,  C12N1/20 ,  C12N15/52 ,  C12N1/16 ,  C12N1/14

CPC classification number: C12N9/20 ,  C11D3/38627 ,  C11D3/38681 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N15/52

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US20220372454A1

公开(公告)日：2022-11-24

申请号：US17816892

申请日：2022-08-02

Applicant: Novozymes A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386 ,  C12N1/20 ,  C12N15/52 ,  C12N1/16 ,  C12N1/14

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US11441134B2

公开(公告)日：2022-09-13

申请号：US17128344

申请日：2020-12-21

Applicant: Novozymes A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386 ,  C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US20180346893A1

公开(公告)日：2018-12-06

申请号：US15580853

申请日：2016-07-01

Applicant: NOVOZYMES A/S

Inventor： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC: C12N9/20 ,  C11D3/386

CPC classification number: C11D3/38627 ,  C11D3/38681 ,  C12N9/20

Abstract: The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US20160319325A1

公开(公告)日：2016-11-03

申请号：US15107654

申请日：2015-01-23

Applicant: NOVOZYMES A/S

Inventor： Yuan Xu ,  Steffen Kayser ,  Bitten Plesner

IPC: C12Q1/44 ,  C11D3/386

CPC classification number: C12Q1/44 ,  C11D3/38627 ,  C12Y301/01003 ,  G01N2333/92

Abstract: The present invention provides a swatch comprising a fabric and a colored lipid stain, wherein said colored lipid stain comprises one or more of several lipase substrates, at least one carotenoid, and optionally a color preserving agent. The present invention also provides a method for preparing the swatch.

Abstract translation: 本发明提供了一种包含织物和着色脂质染色剂的样片，其中所述着色脂质染色剂包括几种脂肪酶底物，至少一种类胡萝卜素和任选的防色剂中的一种或多种。 本发明还提供了一种用于制备样片的方法。