公开(公告)号：US10478116B2

公开(公告)日：2019-11-19

申请号：US15114957

申请日：2015-01-30

Applicant: University of Florida Research Foundation, Inc.

Inventor： Josef Neu ,  Sungho Oh ,  Jui-Hong Chien ,  Eric Ortigoza

IPC: A61B5/04 ,  A61B5/00 ,  A61B5/0488 ,  A61B5/145 ,  A61B7/00 ,  A61B5/0476

Abstract: An aspect of the invention described herein includes a method for a digestion diagnosis of a subject. The method includes obtaining electrogastrogram (EGG), bowel sound and NIRS signals from one or more sensors positioned about an abdominal region of a subject, removing artifacts from the signals to obtain conditioned signal data, and determining a digestion condition or feeding readiness of the subject based at least in part on the conditioned signal data.

公开(公告)号：US20190323069A1

公开(公告)日：2019-10-24

申请号：US16366731

申请日：2019-03-27

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yunwei Charles Cao

IPC: C12Q1/6816 ,  G01N33/58 ,  B01L3/00 ,  G01N33/543

Abstract: Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can include an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.

公开(公告)号：US20190314769A1

公开(公告)日：2019-10-17

申请号：US16382851

申请日：2019-04-12

Applicant: University of Florida Research Foundation, Inc.

Inventor： Saeed Moghaddam

IPC: B01D69/14 ,  B01D67/00 ,  B01D69/02 ,  B01D71/02 ,  B01D71/60

Abstract: Various examples are provided for highly selective and ultra-high throughput filtration using bilayer two-dimensional (2D) material laminates and highly absorptive medium of 2D material laminates or solution dispersions. In one example, a 2D material bilayer membrane includes a first membrane layer; an interlinking layer of interlinking molecules disposed on the first membrane layer; and a second membrane layer disposed on the interlinking layer. The interlinking molecules electrostatically or covalently interlink the second membrane layer and first membrane layer.

公开(公告)号：US20190311263A1

公开(公告)日：2019-10-10

申请号：US16345533

申请日：2017-10-27

Applicant: University of Florida Research Foundation, Inc.

Inventor： Jack D. KENDALL ,  Juan C. NINO

IPC: G06N3/08 ,  G06N3/04 ,  G06N3/063 ,  G11C11/54

Abstract: Memristive learning concepts for neuromorphic circuits are described. In one example case, a neuromorphic circuit includes a first oscillatory-based neuron that generates a first oscillatory signal, a diode that rectifies the first oscillatory signal, and a synapse coupled to the diode and including a long-term potentiation (LTP) memristor arranged in parallel with a long-term depression (LTD) memristor. The circuit further includes a difference amplifier coupled to the synapse that generates a difference signal based on a difference between output signals from the LTP and LTD memristors, and a second oscillatory-based neuron electrically coupled to the difference amplifier that generates a second oscillatory signal based on the difference signal. The circuit also includes a feedback circuit that provides a feedback signal to the LTP and LTD memristors based on a difference or error between a target signal and the second oscillatory signal.

公开(公告)号：US20190300861A1

公开(公告)日：2019-10-03

申请号：US16315675

申请日：2017-07-07

Applicant: University of Florida Research Foundation, Inc.

Inventor： CHRISTOPHER DILLON VULPE ,  AMIN SOBH ,  DAVID MICHAEL FAULKNER ,  ABDERRAHMANE TAGMOUNT ,  MICHAEL FASULLO

IPC: C12N5/16 ,  C12N15/10 ,  C12N15/113 ,  C12N9/06 ,  C12N9/10 ,  G01N33/50 ,  C12N15/90

Abstract: Provided herein are genetically engineered cells containing one or more modulated metabolic genes, where the expression of the modulated metabolic gene(s) can be greater than that of an unmodified control. Also provided herein are methods of making the genetically engineered cells using synergistic activation mediator CRISPR-Cas9. Further provided herein are high throughput assays that can employ the genetically engineered cells provided herein.

公开(公告)号：US10426844B2

公开(公告)日：2019-10-01

申请号：US15246385

申请日：2016-08-24

Applicant: University of Florida Research Foundation, Inc.

Inventor： Mavis Agbandje-McKenna ,  William W. Hauswirth ,  Arun Srivastava ,  Li Zhong

IPC: C12N15/86 ,  A61K48/00 ,  C07K14/705 ,  A61K45/06 ,  C07K14/72

Abstract: Disclosed are capsid-mutated rAAV vectors and methods for their use in gene therapy, and particularly for use in delivering therapeutic transgenes to treat a variety of mammalian diseases and disorders, including dysfunctions and abnormal conditions of the human eye. VP3 capsid proteins comprising a modification of one or more of the surface-exposed tyrosine residues are disclosed, and in particular, VP3 capsid protein comprising tyrosine-to-phenylalanine mutations at positions corresponding to Y444F, Y500F, and Y730F of the wild-type AAV2 sequence. Also provided are rAAV virions and viral particles that comprise such a mutated AAV capsid protein and a nucleic acid molecule that expresses one or more selected therapeutic or reporter transgenes in one or more mammalian cells of interest. Advantageously, the capsid-mutated rAAV vectors and virions disclosed herein afford improved transduction efficiency in a variety of cells, tissues and organs of interest, when compared to their unmodified (i.e., wild-type) rAAV vector counterparts.

公开(公告)号：US20190249695A1

公开(公告)日：2019-08-15

申请号：US16395767

申请日：2019-04-26

Applicant: University of Florida Research Foundation, Inc.

Inventor： SUBRATA ROY ,  NICHOLAS S. CAMPBELL

IPC: F15D1/00 ,  H05H1/24 ,  B64C23/00

CPC classification number: F15D1/0075 ,  B64C23/005 ,  H05H1/2406 ,  H05H2001/2418 ,  Y02T50/166

Abstract: The present disclosure is directed towards electro-fluid transducers that may influence the flow of a fluid in and around a channel. In one such embodiment, a system comprises a first electrode at least partially encapsulated by a first dielectric; a second electrode at least partially encapsulated by a second dielectric, wherein a portion of a channel exists between the first dielectric and the second dielectric; a third electrode positioned in the channel; and a fourth electrode positioned in the channel, wherein the electrodes influence a flow of a fluid in the channel upon the electrodes being energized.

公开(公告)号：US20190218264A1

公开(公告)日：2019-07-18

申请号：US16339585

申请日：2017-10-04

Applicant: University of Florida Research Foundation, Inc.

Inventor： GREGORY HUDALLA ,  SABRINA FREEMAN ,  ANTONIETTA RESTUCCIA ,  MARGARET FETTIS ,  EVELYN R. BRACHO-SANCHEZ ,  SHAHEEN FARHADI ,  BENJAMIN G. KESELOWSKY

IPC: C07K14/47 ,  C12N9/02

CPC classification number: C07K14/4726 ,  A61K38/00 ,  C07K14/00 ,  C07K2319/33 ,  C07K2319/73 ,  C12N9/0069 ,  C12N15/00 ,  C12Y113/11052 ,  C12Y113/12007

Abstract: Provided herein are targeted effector fusion proteins, complexes thereof, and uses thereof. The targeted effector fusion proteins can include an effector protein that can be linked to a targeting moiety. Monomer targeted effector fusion proteins can form homogeneous or heterogeneous complexes. The targeted effector fusion proteins and complexes thereof can be formulated as pharmaceutical formulations. The targeted effector fusion proteins, complexes thereof, and formulations thereof can be administered to a subject in need thereof.

公开(公告)号：US10350185B2

公开(公告)日：2019-07-16

申请号：US14773960

申请日：2014-03-11

Applicant: University of Florida Research Foundation, Inc.

Inventor： Sadasivan Vidyasagar ,  Paul Okunieff

IPC: A61K33/14 ,  A61K9/00 ,  A61K31/198 ,  A61K31/405

Abstract: The subject invention provides therapeutic compositions and uses thereof for improving pulmonary function. In one embodiment, the therapeutic composition comprises one or more free amino acids selected from lysine, glycine, threonine, valine, tyrosine, aspartic acid, isoleucine, tryptophan, asparagine, and serine; and electrolytes. In one embodiment, the subject invention can be used to prevent or treat long-term lung complications induced by radiation.

公开(公告)号：US10343219B2

公开(公告)日：2019-07-09

申请号：US15123172

申请日：2015-03-04

Applicant: University of Florida Research Foundation, Inc. ,  UTBATTELLE, LLC

Inventor： Michele Viola Manuel ,  Hunter B. Henderson ,  Orlando Rios ,  Gerard M. Ludtka

IPC: B22F9/06 ,  B22F1/00 ,  C22C1/10 ,  H01F1/047 ,  H01F1/44 ,  H05B6/36 ,  B22F9/04 ,  H01F1/00

Abstract: Disclosed herein is a method comprising disposing a container containing a metal and/or ferromagnetic solid and abrasive particles in a static magnetic field; where the container is surrounded by an induction coil; activating the induction coil with an electrical current, to heat up the metallic or ferromagnetic solid to form a fluid; generating sonic energy to produce acoustic cavitation and abrasion between the abrasive particles and the container; and producing nanoparticles that comprise elements from the container, the metal and/or the ferromagnetic solid and the abrasive particles. Disclosed herein too is a composition comprising first metal or a first ceramic; and particles comprising carbides and/or nitrides dispersed therein. Disclosed herein too is a composition comprising nanoparticles comprising chromium carbide, iron carbide, nickel carbide, y.-Fe and magnesium nitride.