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    Use of circulating tumor cell mitotic index in cancer stratification and diagnostics
    11.
    发明授权
    Use of circulating tumor cell mitotic index in cancer stratification and diagnostics 有权

    公开(公告)号：US11150245B2

    公开(公告)日：2021-10-19

    申请号：US15576472

    申请日：2016-05-26

    Applicant: CREATV MICROTECH INC.

    Inventor： Daniel Adams ,  Cha-Mei Tang

    IPC: G01N33/574 ,  G01N33/50

    Abstract: Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.

    POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES
    16.
    发明申请
    POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES 审中-公开

    公开(公告)号：US20190324014A1

    公开(公告)日：2019-10-24

    申请号：US16400600

    申请日：2019-05-01

    Applicant: Creatv MicroTech, Inc.

    Inventor： Cha-Mei TANG

    IPC: G01N33/50 ,  B01D39/16 ,  B01L3/00 ,  B01L9/00 ,  B01D29/00 ,  G01N1/40

    Abstract: A microfilter comprising a polymer layer formed from photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. Methods of forming apertures in one or more layers of photo-definable dry film are also disclosed. Filter holder designs and methods appropriate to hold microfilters to collect the rare cells and to perform of assays in the filter holder are provided. Microfiltration chip designs and methods appropriate to collect the rare cells and to perform assays in the microfluidic chips are provided. The invention also describes the use of the microfilter, filter holder and microfilter chips to collect rare cells from body fluids and perform assays, and these rare cells can be used for medical and biological research applications.

    POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES
    18.
    发明申请
    POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES 审中-公开
    聚合物微滤装置，其制造方法和微滤装置的用途

    公开(公告)号：US20140322743A1

    公开(公告)日：2014-10-30

    申请号：US14359467

    申请日：2012-11-21

    Applicant: Creatv Microtech, Inc.

    Inventor： Cha-Mei Tang ,  Yunqi Zhang

    IPC: G01N33/50 ,  B01D39/16 ,  B01L3/00 ,  B01L9/00

    CPC classification number: G01N33/5005 ,  B01D29/00 ,  B01D39/16 ,  B01D39/1692 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5635 ,  B01L9/00 ,  B01L2300/0681 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  Y10T29/49826 ,  Y10T29/49963

    Abstract: A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from epoxy-based photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate. Unique filter holder designs and methods appropriate to hold microfilters to collect the rare cells and allow performing assays in the filter holder are provided. The invention also describes the use of the microfilter and filter holder to collect rare cells from body fluids and perform assays. Rare cells collected on the microfilter in accordance with embodiments of the present invention can be used for medical and biological research applications.

    Abstract translation: 一种包含由环氧基可光定影干膜形成的聚合物层的微过滤器，以及遍及聚合物层的多个孔。 一种包含由环氧基可光限制的干膜形成的两个或更多个聚合物层的微过滤器，以及各自延伸穿过聚合物层的多个孔或开口区域。 还公开了一种形成微过滤器的方法。 该方法包括提供设置在基底上的第一层环氧基光可定义干膜，将第一层暴露于能量通过掩模，以在第一层干膜中形成由掩模限定的图案， 从曝光的第一层干膜，具有延伸穿过其中的多个孔的聚合物层，所述多个孔具有由图案限定的分布，以及从基底去除聚合物层。 提供了独特的过滤器支架设计和适用于容纳微过滤器以收集稀有细胞并允许在过滤器保持器中进行测定的方法。 本发明还描述了微过滤器和过滤器保持器从体液中收集稀有细胞并进行测定的用途。 根据本发明的实施方案收集在微过滤器上的稀有细胞可用于医学和生物研究应用。

    Apparatus and method for three-dimensional image reconstruction
    19.
    发明申请
    Apparatus and method for three-dimensional image reconstruction 失效
    三维图像重建的装置和方法

    公开(公告)号：US20030228053A1

    公开(公告)日：2003-12-11

    申请号：US10429258

    申请日：2003-05-05

    Applicant: Creatv MicroTech, Inc.

    Inventor： Shuhong Li ,  Cha-Min Tang ,  Cha-Mei Tang

    IPC: G06T007/00

    CPC classification number: G06T7/55 ,  G06T2207/10056 ,  G06T2207/30024

    Abstract: An instrument to acquire and methods to obtain three-dimensional (3D) images from a series of two-dimensional (2D) images which are obtained without moving the relative positions of the target, the detector, or the focusing lens is disclosed. The 2D images consist of one centered image obtained with the aperture at the center of optical system, and at least two directional images obtained with apertures at off-axis locations. The images can be obtained simultaneously or sequentially. The blurred 2D images are sectioned by computational method using point spread function of the optical system resulting in a set of decoupled 2D layers of the 3D object. The layered images are then sharpened by deconvolution using point spread function. The 3D reconstructed image is displayed. This technique provides fast data acquisition and fast image reconstruction and eliminates problems associated with motion, phototoxicity and photobleaching.

    Abstract translation: 公开了一种用于获取并从一系列二维（2D）图像中获得三维（3D）图像的仪器，该图像在不移动目标，检测器或聚焦透镜的相对位置的情况下获得。 2D图像由在光学系统的中心处的孔径获得的一个中心图像和在离轴位置处具有孔径获得的至少两个定向图像组成。 图像可以同时或顺序获得。 通过使用光学系统的点扩散函数的计算方法对模糊的2D图像进行分割，得到3D对象的一组去耦2D层。 然后通过使用点扩散函数的去卷积来锐化分层图像。 显示3D重建图像。 该技术提供快速数据采集和快速图像重建，并消除与运动，光毒性和光漂白相关的问题。

    FREESTANDING HIGH-ASPECT-RATIO GOLD MASKS FOR LOW-ENERGY, PHASE-BASED X-RAY MICROSCOPY
    20.
    发明公开
    FREESTANDING HIGH-ASPECT-RATIO GOLD MASKS FOR LOW-ENERGY, PHASE-BASED X-RAY MICROSCOPY 审中-公开

    公开(公告)号：US20240312665A1

    公开(公告)日：2024-09-19

    申请号：US18377523

    申请日：2023-10-06

    Applicant: Creatv MicroTech, Inc.

    Inventor： Olga V. Makarova ,  Ralu Divan ,  Nicolaie Moldovan ,  David A. Czaplewski ,  Cha-Mei Tang

    IPC: G21K1/10 ,  G21K7/00

    CPC classification number: G21K1/10 ,  G21K7/00

    Abstract: High-resolution, X-ray phase contrast microscopy, a key technique with promising potential in biomedical imaging and diagnostics, is based on narrow-slit high-aspect-ratio gold gratings. We present the development, fabrication details, and experimental testing of the freestanding 10-μm-thick gold membrane masks with an array of 0.9-1.5 □m void slit apertures for a novel low-energy X-ray microscope. The overall mask size is 4 mm×4 mm, with a grating pitch of 7.5 □m, 6.0-6.6-□m-wide gold bars are supported by 3-□m-wide crosslinks at 400 □m intervals. The fabrication process is based on gold electroplating into a silicon mold coated with various thin films to form a voltage barrier, plating base, and sacrificial layer, followed by the mold removal to obtain the freestanding gold membrane with void slit apertures. We discuss key aspects for the materials and processes, including gold structures homogeneity, residual stresses, and prevention of collapsing of the grid elements. We further demonstrate the possibility to obtain high-resolution, high contrast 2D images of biological samples using an incoherent, rotating anode X-ray tube.

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