公开(公告)号：US08828399B2

公开(公告)日：2014-09-09

申请号：US13409987

申请日：2012-03-01

申请人： Thomas Shenk ,  Dai Wang

发明人： Thomas Shenk ,  Dai Wang

IPC分类号： C07K16/08 ,  A61K39/42 ,  A61K39/395 ,  C12N5/12

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/505 ,  C07K14/005 ,  C07K16/085 ,  C07K16/088 ,  C07K2317/76 ,  C12N7/00 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12Q1/025 ,  G01N2500/04

摘要： Immunogenic compositions and prophylactic or therapeutic vaccines for use in protecting and treating against human cytomegalovirus (CMV) are disclosed. Subunit vaccines comprising a human CMV protein complex comprising pUL128 or pUL130, and nucleic acid vaccines comprising at least one nucleic acid encoding a CMV protein complex comprising pUL128 or pUL130 are described. Also disclosed are therapeutic antibodies reactive against a CMV protein complex comprising pUL128 or pUL130, as well as methods for screening compounds that inhibit CMV infection of epithelial and endothelial cells, methods for immunizing a subject against CMV infection, methods for determining the capability of neutralizing antibodies to inhibit CMV infection of cell types other than fibroblasts, and methods of diminishing an CMV infection.

摘要翻译： 公开了用于保护和治疗人巨细胞病毒（CMV）的免疫原性组合物和预防或治疗性疫苗。 描述了包含包含pUL128或pUL130的人CMV蛋白复合物的亚单位疫苗和包含至少一种编码包含pUL128或pUL130的CMV蛋白质复合物的核酸的核酸疫苗。 还公开了对包含pUL128或pUL130的CMV蛋白质复合物具有反应性的治疗性抗体以及用于筛选抑制上皮和内皮细胞的CMV感染的化合物的方法，用于免疫CMV感染的受试者的方法，用于测定中和抗体的能力的方法 抑制除成纤维细胞以外的细胞类型的CMV感染，以及减少CMV感染的方法。

公开(公告)号：US08173362B2

公开(公告)日：2012-05-08

申请号：US12766611

申请日：2010-04-23

申请人： Thomas Shenk ,  Dai Wang

发明人： Thomas Shenk ,  Dai Wang

IPC分类号： C12Q1/70

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/505 ,  C07K14/005 ,  C07K16/085 ,  C07K16/088 ,  C07K2317/76 ,  C12N7/00 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12Q1/025 ,  G01N2500/04

摘要： Immunogenic compositions and prophylactic or therapeutic vaccines for use in protecting and treating against human cytomegalovirus (CMV) are disclosed. Subunit vaccines comprising a human CMV protein complex comprising pUL128 or pUL130, and nucleic acid vaccines comprising at least one nucleic acid encoding a CMV protein complex comprising pUL128 or pUL130 are described. Also disclosed are therapeutic antibodies reactive against a CMV protein complex comprising pUL128 or pUL130, as well as methods for screening compounds that inhibit CMV infection of epithelial and endothelial cells, methods for immunizing a subject against CMV infection, methods for determining the capability of neutralizing antibodies to inhibit CMV infection of cell types other than fibroblasts, and methods of diminishing an CMV infection.

摘要翻译： 公开了用于保护和治疗人巨细胞病毒（CMV）的免疫原性组合物和预防或治疗性疫苗。 描述了包含包含pUL128或pUL130的人CMV蛋白复合物的亚单位疫苗和包含至少一种编码包含pUL128或pUL130的CMV蛋白质复合物的核酸的核酸疫苗。 还公开了对包含pUL128或pUL130的CMV蛋白质复合物具有反应性的治疗性抗体以及用于筛选抑制上皮和内皮细胞的CMV感染的化合物的方法，用于免疫CMV感染的受试者的方法，用于测定中和抗体的能力的方法 抑制除成纤维细胞以外的细胞类型的CMV感染，以及减少CMV感染的方法。

公开(公告)号：US20100285059A1

公开(公告)日：2010-11-11

申请号：US12681504

申请日：2008-10-01

申请人： Thomas Shenk ,  Dai Wang

发明人： Thomas Shenk ,  Dai Wang

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/54 ,  C12N7/00 ,  C12N2710/16134 ,  C12N2710/16151 ,  C12N2710/16164

摘要： Methods of increasing diversity in cytomegalovirus vaccines through the selection of cell type in which the virus is propagated, and the use of cytomegalovirus produced by those methods in the development of vaccine compositions, are disclosed. Vaccine compositions comprising CMV isolated from epithelial cells are also disclosed.

摘要翻译： 公开了通过选择扩增病毒的细胞类型增加巨细胞病毒疫苗多样性的方法，以及通过这些方法产生的巨细胞病毒在开发疫苗组合物中的应用。 还公开了包含从上皮细胞分离的CMV的疫苗组合物。

公开(公告)号：US20100144781A1

公开(公告)日：2010-06-10

申请号：US12608386

申请日：2009-10-29

申请人： Dong-Jing Fu ,  Qingqin S. Li ,  Dai Wang

发明人： Dong-Jing Fu ,  Qingqin S. Li ,  Dai Wang

IPC分类号： A61K31/4375 ,  C12Q1/68

CPC分类号： A61K31/4375 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172

摘要： The present application is directed to the use of genetic polymorphism in the ErbB4 gene to predict whether a patient is likely to respond to psychotic medication Paliperidone. The polymorphism in the ErbB4 gene is also used to predict whether a patient is likely to display placebo effect among patients in need of psychotic treatment. A method of treating patients with antipsychotic medication Paliperidone using the polymorphism in the ErbB4 gene and a kit of are also provided.

摘要翻译： 本申请涉及在ErbB4基因中使用遗传多态性来预测患者是否可能对精神病药物帕利哌酮有反应。 ErbB4基因多态性也用于预测患者是否可能在需要精神病治疗的患者中显示安慰剂效应。 一种治疗患有抗精神病药物的方法也提供了使用ErbB4基因中的多态性的一种试剂盒。

公开(公告)号：US20230399648A1

公开(公告)日：2023-12-14

申请号：US18251314

申请日：2021-11-08

申请人： Dai WANG ,  John P. BILELLO ,  Kevin B. GURNEY ,  Ping HAN ,  Xi HE ,  Dengyun SUN ,  Amy Hsu TOU ,  MERCK SHARP & DOHME LLC

发明人： Dai WANG ,  John P. BILELLO ,  Kevin B. GURNEY ,  Ping HAN ,  Xi HE ,  Dengyun SUN ,  Amy Hsu TOU

IPC分类号： C12N15/63 ,  C12N7/00 ,  C12Q1/6897 ,  C12Q1/66

CPC分类号： C12N15/63 ,  C12N7/00 ,  C12Q1/6897 ,  C12Q1/66

摘要： The disclosure describes methods for measuring the potency of anti-Respiratory Syncytial Virus (RSV) antibody against respiratory syncytial virus in a cell culture system using an RSV reporter virus. The disclosure also describes respiratory syncytial viruses that carry a reporter gene, and expression vectors for producing an infectious recombinant respiratory syncytial virus (RSV) that carry a reporter gene.

公开(公告)号：US09546355B2

公开(公告)日：2017-01-17

申请号：US14343637

申请日：2012-09-04

申请人： Tong-Ming Fu ,  Dai Wang ,  Muneeswara Babu Medi

发明人： Tong-Ming Fu ,  Dai Wang ,  Muneeswara Babu Medi

IPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/55505 ,  A61K2039/55577 ,  C12N2710/16134

摘要： The present invention relates to methods of inducing an immune response to cytomegalovirus (CMV) using a genetically modified CMV that is conditionally replication defective. The methods of the invention can be used to treat and/or prevent primary CMV infection, infection due to reactivation of a latent CMV and a super-infection of a different strain of CMV that had been previously encountered. The present invention also relates to a replication defective CMV which has been recombinantly altered to allow for external control of viral replication. Compositions comprising the replication defective CMV are also encompassed by the present invention.

摘要翻译： 本发明涉及使用有条件复制缺陷的遗传修饰的CMV诱导对巨细胞病毒（CMV）的免疫应答的方法。 本发明的方法可用于治疗和/或预防原发性CMV感染，由于潜在CMV的再活化引起的感染和先前已经遇到的不同CMV株的超感染。 本发明还涉及重组改变以​​允许外部控制病毒复制的复制缺陷型CMV。 包含复制缺陷型CMV的组合物也包括在本发明中。