公开(公告)号：US5512459A

公开(公告)日：1996-04-30

申请号：US95162

申请日：1993-07-20

申请人： Fred W. Wagner ,  Jay Stout ,  Dennis Henriksen ,  Bruce Partridge ,  Shane Manning

发明人： Fred W. Wagner ,  Jay Stout ,  Dennis Henriksen ,  Bruce Partridge ,  Shane Manning

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/04 ,  A61P3/08 ,  A61P13/02 ,  A61P15/00 ,  A61P43/00 ,  C07K14/60 ,  C07K14/605 ,  C12N1/21 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19 ,  C12N9/74 ,  C12N9/76

CPC分类号： C07K14/60 ,  C07K14/605 ,  C12P21/02 ,  C12P21/06 ,  A61K38/00 ,  C07K2319/00

摘要： The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously under go substitution of the leaving unit by the desired addition unit. The invention utilizes known and newly discovered cleavage recognition sites to effectuate the desired modification products.

摘要翻译： 本发明的方法提供了通过使用转肽酶方法在C末端修饰的重组多肽的形成。 该方法适用于修饰任何来源的重组多肽，包括可商购的重组多肽，衍生自重组单拷贝或多拷贝多肽构建体的重组多肽，或衍生自单拷贝或多拷贝重组融合蛋白构建体的重组多肽。 转肽反应包括使内肽酶与重组多肽接触，以将一个或多个氨基酸的加成单元替换为通过内肽酶识别的切割位点与核心多肽连接的离去单元。 衍生自多拷贝多肽构建体的重组多肽可以在N末端和C-末端的多拷贝多肽中切割，同时在离去单位取代所需的加成单位。 本发明利用已知和新发现的裂解识别位点来实现所需的修饰产物。

公开(公告)号：US08493978B2

公开(公告)日：2013-07-23

申请号：US13122517

申请日：2009-09-30

申请人： Marta San Martin Arribas ,  Dennis Henriksen ,  Jorge Nevado Jimenez

发明人： Marta San Martin Arribas ,  Dennis Henriksen ,  Jorge Nevado Jimenez

IPC分类号： H04L12/28

CPC分类号： H04L67/1095 ,  H04L67/1002 ,  H04L67/1008 ,  H04L67/101 ,  H04L67/1097

摘要： A transmitting node in a telecommunications network, wherein data are distributed into one or more data storage groups (DSG), and wherein data related to a DSG is replicated into a plurality of data storage elements (DS) distributed along one or more nodes of the network. The transmitting node includes a network interface through which a bi-directional connection is established with a plurality of other nodes in a transport layer of the network, and from which messages are sent to the plurality of other nodes comprising an operational state matrix (OSM), which includes information held by the transmitting node about the operational state of the replicas of the DSs of each DSG. The transmitting node includes a memory for storing data. The transmitting node includes a processing unit which forms a list of the plurality of other nodes as having an answer pending of the node in the memory. The network interface receives an OSM from at least some of other nodes which are interpreted by the processing unit as answer messages from the list of the plurality of other nodes to the messages sent by the network interface, and which indicates that an OSM information exchange with the plurality of the other nodes is complete. The processing unit decides a node hosting the master DS replica of each DSG based on the OSM information exchange. The processing unit detects changes in a view of the world (VOW) which includes an accumulated set of OSMs with respect to each DSG hosted by the transmitting node. The network interface sends messages comprising changes in the VOW to the other nodes.

摘要翻译： 电信网络中的发送节点，其中数据被分配到一个或多个数据存储组（DSG）中，并且其中与DSG相关的数据被复制到沿着所述数据存储组（DSG）的一个或多个节点分发的多个数据存储元件 网络。 发射节点包括网络接口，通过该网络与网络的传输层中的多个其他节点建立双向连接，并且从哪个消息发送到包括操作状态矩阵（OSM）的多个其他节点， ，其包括发送节点关于每个DSG的DS的副本的操作状态的信息。 发送节点包括用于存储数据的存储器。 发送节点包括处理单元，该处理单元将多个其他节点的列表形成为具有存储器中节点的待决答案。 网络接口从至少一些其他节点接收OSM，这些节点由处理单元解释为从多个其他节点的列表到由网络接口​​发送的消息的应答消息，并且其指示OSM信息与 多个其他节点是完整的。 处理单元基于OSM信息交换来决定承载每个DSG的主DS副本的节点。 处理单元检测世界视图（VOW）中的变化，其包括相对于由发送节点托管的每个DSG的累积的OSM集合。 网络接口将包含VOW中的更改的消息发送到其他节点。