摘要:
The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
摘要:
Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
摘要:
The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
摘要:
Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mt, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
摘要:
In a CMOS SRAM cell formed on an SOI substrate and including a flip-flop having first and second NMOS and PMOS transistors, transfer gates having first and seconf MOS transistors, and a word line section, characterized in that:the word line section extends along a predetermined direction; that source and drain diffusion layer regions of the first and second NMOS and PMOS transistors are arranged along the predetermined direction, and gates of these NMOS and PMOS transistors are arranged on channel regions thereof in a direction perpendicular to the predetermined direction; that the gates of the first and second NMOS transistors are electrically connected to the gates of the first and second PMOS transistors, respectively; and that in regions between the gates of the first and second NMOS transistors on the channel regions and the gates of the first and second PMOS transistors on the channel regions, each of the drain diffusion layer regions of the fisrt and second NMOS and PMOS transistors, and each one of the drain and source diffusion layer regions of the first and second MOS transistors are respectively arranged to be adgacent to each other and are electrically connected to each other, respectively, through a diffusion layer interconnection.
摘要:
Plural nonvolatile address storing circuits hold address data. A serial transfer circuit sequentially transfers the address data stored in each of the nonvolatile address storing circuits. A serial reception circuit sequentially receives the address data transferred by the serial transfer circuit. An address latch circuit holds the address data received by the serial reception circuit. An address comparison circuit compares each of the address data stored in the address latch circuit with an input address, and determines whether each address data coincides with the input address.
摘要:
An address generating circuit includes a first carry look-ahead circuit for performing an operation using an inputted first carry and a first address and outputting a first output address and a first output carry respectively as a first operation result, a second carry look-ahead circuit for performing an operation using a carry fixed to 0 and a second address and outputting a second output address and a second output carry respectively as a second operation result, a third carry look-ahead circuit for performing an operation using a carry fixed to 1 and the second address and outputting a third output address and a third output carry respectively as a third operation result, and a first select circuit for selecting either of the second and third operation results based on the first output carry and outputting the selected operation result.
摘要:
The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.