公开(公告)号：US20060260008A1

公开(公告)日：2006-11-16

申请号：US11485079

申请日：2006-07-12

申请人： Mark Rupar ,  William Donovan ,  Chih-Rei Chu ,  Elizabeth Pease ,  Yuping Tan ,  Annette Slaney ,  Thomas Malvar ,  James Baum

发明人： Mark Rupar ,  William Donovan ,  Chih-Rei Chu ,  Elizabeth Pease ,  Yuping Tan ,  Annette Slaney ,  Thomas Malvar ,  James Baum

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  C12N1/21 ,  C07K14/325

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

摘要翻译： 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。

公开(公告)号：US20060191034A1

公开(公告)日：2006-08-24

申请号：US10563270

申请日：2004-07-06

申请人： James Baum ,  Judith Donovan ,  William Donovan ,  James Engleman ,  Karina Krasomil-Osterfeld ,  John Pitkin ,  James Roberts

发明人： James Baum ,  Judith Donovan ,  William Donovan ,  James Engleman ,  Karina Krasomil-Osterfeld ,  John Pitkin ,  James Roberts

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C07K14/325 ,  A01N63/00

CPC分类号： A01N63/02 ,  C07K14/32

摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。 公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。