公开(公告)号：US06812380B2

公开(公告)日：2004-11-02

申请号：US10107096

申请日：2002-03-26

申请人： Petr Karlovsky ,  Edmund H. Crane, III ,  Jacob T. Gilliam ,  Joyce R. Maddox

发明人： Petr Karlovsky ,  Edmund H. Crane, III ,  Jacob T. Gilliam ,  Joyce R. Maddox

IPC分类号： C12N1509

CPC分类号： C12N9/18

摘要： The present invention provides nucleotide sequences encoding zearalenone detoxification polypeptides, and methods for mycotoxin detoxification using the sequences. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供了编码玉米赤霉烯酮解毒多肽的核苷酸序列，以及使用该序列的霉菌毒素解毒的方法。 一种方法包括稳定地并入植物细胞的基因组中，本发明的核苷酸序列可操作地连接到异源启动子并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US06627797B1

公开(公告)日：2003-09-30

申请号：US09810268

申请日：2001-03-16

申请人： Jon Duvick ,  Joyce R. Maddox ,  Nancy P. Keller

发明人： Jon Duvick ,  Joyce R. Maddox ,  Nancy P. Keller

IPC分类号： C12N1509

CPC分类号： C12N15/8247 ,  C12N15/8243 ,  C12N15/8282

摘要： The invention provides isolated maize lipoxygenase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering lipoxygenase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

摘要翻译： 本发明提供了分离的玉米脂氧合酶核酸及其编码的蛋白质。 本发明提供了与改变植物脂氧合酶浓度和/或组成有关的方法和组合物。 本发明还提供了重组表达盒，宿主细胞和转基因植物。

公开(公告)号：US06239330B1

公开(公告)日：2001-05-29

申请号：US09262758

申请日：1999-03-04

申请人： Jonathan Duvick ,  Joyce R. Maddox ,  Xun Wang

发明人： Jonathan Duvick ,  Joyce R. Maddox ,  Xun Wang

IPC分类号： C12N1531

CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

公开(公告)号：US06229071B1

公开(公告)日：2001-05-08

申请号：US08888950

申请日：1997-07-07

申请人： Jonathan Duvick ,  Joyce R. Maddox ,  Tracy A. Rood ,  Xun Wang

发明人： Jonathan Duvick ,  Joyce R. Maddox ,  Tracy A. Rood ,  Xun Wang

IPC分类号： A01H106

CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

公开(公告)号：US5792931A

公开(公告)日：1998-08-11

申请号：US484815

申请日：1995-06-07

申请人： Jonathan Duvick ,  Tracy Rood ,  Joyce R. Maddox ,  Xun Wang

发明人： Jonathan Duvick ,  Tracy Rood ,  Joyce R. Maddox ,  Xun Wang

IPC分类号： C12N15/09 ,  A01N63/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/00 ,  C12N15/55 ,  C12N15/82 ,  C12R1/645 ,  C12S3/00 ,  A01H5/00

CPC分类号： A01N63/00 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18

摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on flumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for furaonisin-resistance can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain processing and in animal feed and rumen microbes are also disclosed.

摘要翻译： 鉴定能够降解伏马菌素的生物体的方法。 可以将鱼藤苷加入培养基中，以选择对伏马菌素具有抗性的生物体和/或能够在作为唯一碳源的烟曲霉毒素上生长。 使用这种方法，已经确定了几种生物体。 这些生物可用于分离酶和负责赋予伏马菌素抗性的基因。 可以将该基因克隆并插入到合适的表达载体中，以使蛋白质进一步表征。 此外，编码呋喃诺森抗性的DNA可用于转化通常易受镰刀菌或其它产生毒素的真菌感染的植物细胞。 植物可以从转化的植物细胞中再生。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 还公开了谷物加工和动物饲料和瘤胃微生物中的解毒方法。