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    Sequential Sequencing
    14.
    发明申请
    Sequential Sequencing 审中-公开
    顺序排序

    公开(公告)号：US20160251712A1

    公开(公告)日：2016-09-01

    申请号：US14990339

    申请日：2016-01-07

    申请人： NuGEN Technologies, Inc.

    发明人： Doug Amorese ,  Benjamin G. Schroeder ,  Jonathan Scolnick

    IPC分类号： C12Q1/68 ,  G06F19/22

    摘要： The present invention provides improved methods, compositions and kits for short read next generation sequencing (NGS). The methods, compositions and kits of the present invention enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (typically comprising regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information is obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein are useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.

    摘要翻译： 本发明提供用于短读下一代测序（NGS）的改进的方法，组合物和试剂盒。 本发明的方法，组合物和试剂盒能够使样品中的两个或多个核酸序列相位定位，即确定核酸序列（通常包含序列变异区域）是否位于同一染色体和/或相同染色体上 分段。 通过从相同的固定化核酸模板进行多个连续的测序反应获得相位信息。 本文提供的方法，组合物和试剂盒可用于例如单倍型，SNP定相或用于确定RNA-seq中的下游外显子。

    SEQUENTIAL SEQUENCING
    17.
    发明申请
    SEQUENTIAL SEQUENCING 审中-公开
    顺序排序

    公开(公告)号：US20140274738A1

    公开(公告)日：2014-09-18

    申请号：US14211261

    申请日：2014-03-14

    申请人： NuGEN Technologies, Inc.

    发明人： Doug Amorese ,  Benjamin G. Schroeder ,  Jonathan Scolnick

    IPC分类号： C12Q1/68 ,  G06F19/22

    CPC分类号： C12Q1/6874 ,  G16B30/00

    摘要： The present invention provides improved methods, compositions and kits for short read next generation sequencing (NGS). The methods, compositions and kits of the present invention enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (typically comprising regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information is obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein are useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.

    摘要翻译： 本发明提供用于短读下一代测序（NGS）的改进的方法，组合物和试剂盒。 本发明的方法，组合物和试剂盒能够使样品中的两个或多个核酸序列相位定位，即确定核酸序列（通常包含序列变异区域）是否位于同一染色体和/或相同染色体上 分段。 通过从相同的固定化核酸模板进行多个连续的测序反应获得相位信息。 本文提供的方法，组合物和试剂盒可用于例如单倍型，SNP定相或用于确定RNA-seq中的下游外显子。

    Library preparation
    18.
    发明授权
    Library preparation 有权

    公开(公告)号：US11365408B2

    公开(公告)日：2022-06-21

    申请号：US16268971

    申请日：2019-02-06

    申请人： NUGEN TECHNOLOGIES, INC.

    发明人： Bin Li ,  Benjamin G. Schroeder ,  Manqing Hong ,  Maureen Peterson

    IPC分类号： C12N15/10 ,  C40B50/06 ,  C40B40/06

    摘要： The disclosure provides DNA library preparation methods that do not require a purification between adapter ligation and PCR amplification. Adaptors are added to DNA fragments to form oligonucleotide extension products and the oligonucleotide extension products are amplified without stopping or interruption for a cleanup step. Excess materials, such as enzymes, adaptors, or co-factors, from the adaptor addition step do not interfere with the amplification step and the amplification step proceeds without regards to the presence of reagents from the ligation step. In preferred embodiments, the ligation and amplification step make use of a common priming sequence e.g., in the form of one of the adaptor oligos.