公开(公告)号：US20060068396A1

公开(公告)日：2006-03-30

申请号：US10948224

申请日：2004-09-24

Applicant: Shiu-Ru Lin ,  Tian-Lu Cheng ,  Yi-Fang Chen ,  Chan-Han Wu

Inventor： Shiu-Ru Lin ,  Tian-Lu Cheng ,  Yi-Fang Chen ,  Chan-Han Wu

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q2565/507

Abstract: The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon membrane chip by a rapid nucleic acid cross-linker when preparing a chip; collecting some normal whole blood to be linearly amplified; synthesizing required amount of cDNA by a reverse transcription to obtain a marker as a probe; processing labeling, hybridization and post-hybridization to the chip and the marker; processing chemical color reaction; and automatically analyzing the result image after the chemical color reaction. Accordingly, a gene biochip operation technology platform with low cost, easy operation and high efficiency is obtained. And so the functions and the applications of the gene biochip can be effectively worked out and the practical applications of the gene biochip on related fields can be conclusively popularized.

Abstract translation: 本发明是一种检测有限细胞的生物芯片的方法。 本发明包括以下步骤：通过使用手动打斑机获得具有以点阵方式布置的所需核苷酸片段的尼龙膜芯片; 通过加热自然干燥，并在制备芯片时通过快速核酸交联剂固定尼龙膜芯片上的核苷酸片段; 收集一些正常全血要线性扩增; 通过逆转录合成所需量的cDNA以获得作为探针的标记; 加工标记，杂交和后期杂交到芯片和标记物; 加工化学色素反应; 并在化学反应后自动分析结果图像。 因此，获得了低成本，易于操作和高效率的基因生物芯片操作技术平台。 因此可以有效地制定基因生物芯片的功能和应用，基因生物芯片在相关领域的实际应用可以得到最终推广。

公开(公告)号：US20110281770A1

公开(公告)日：2011-11-17

申请号：US12849264

申请日：2010-08-03

Applicant: Shiu-Ru Lin ,  Hui-Jen Chang ,  Suz-Kai Hsiung ,  Chan-Han Wu

Inventor： Shiu-Ru Lin ,  Hui-Jen Chang ,  Suz-Kai Hsiung ,  Chan-Han Wu

IPC: C40B40/08

CPC classification number: C12Q1/6837 ,  G01N35/0098 ,  G01N35/0099 ,  G01N2035/00158 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/00564

Abstract: A sample is diagnosed with a genechip array by the present disclosure. The sample is automatically diagnosed by devices revealed in the present disclosure for a fast diagnosis having high accuracy.

Abstract translation: 通过本公开将样品诊断为基因芯片阵列。 通过本公开中揭示的装置自动诊断样品，用于具有高精度的快速诊断。