公开(公告)号：US08357782B2

公开(公告)日：2013-01-22

申请号：US12933768

申请日：2009-03-16

Applicant: Shigeharu Fukuda ,  Chie Ushio ,  Harumi Ariyasu ,  Tohru Kayano ,  Toshio Ariyasu ,  Tsunetaka Ohta

Inventor： Shigeharu Fukuda ,  Chie Ushio ,  Harumi Ariyasu ,  Tohru Kayano ,  Toshio Ariyasu ,  Tsunetaka Ohta

IPC: C07K16/24 ,  A61K39/395 ,  C12N5/12

CPC classification number: C07K16/249 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/76 ,  G01N33/6866 ,  G01N2030/8831

Abstract: The present invention has the first object to provide a monoclonal antibody specific to interferon α subtype α8 (IFNα8) and its mutant proteins, the second object to provide a hybridoma capable of producing the monoclonal antibody, the third object to provide a method for detecting the IFNα8 and its mutant proteins by the monoclonal antibody, the fourth object to provide a method for purifying the IFNα8 and its mutant proteins by the monoclonal antibody, and the fifth object to provide a therapeutic agent for treating diseases whose onsets or exacerbation are related with IFNα8. The present invention solves the above objects by providing a monoclonal antibody specific to IFNα8 and its mutant proteins, a hybridoma capable of producing the monoclonal antibody, a method for detecting the IFNα8 and its mutant proteins by immunoreaction using the monoclonal antibody, a method for purifying the IFNα8 and its mutant proteins using the monoclonal antibody, and a therapeutic agent for treating diseases whose onsets or exacerbation are related with IFNα8, which contains the monoclonal antibody as an effective ingredient.

Abstract translation: 本发明的第一个目的是提供对干扰素α亚型α8（IFNα8）及其突变蛋白特异性的单克隆抗体，第二个目的是提供能产生单克隆抗体的杂交瘤，第三个目的是提供一种检测方法 通过单克隆抗体IFNα8及其突变蛋白，第四个目的是提供通过单克隆抗体纯化IFNα8及其突变蛋白的方法，第五个目的是提供一种用于治疗其发作或加重与IFNα8相关的疾病的治疗剂 。 本发明通过提供对IFNα8及其突变蛋白特异性的单克隆抗体，能够产生单克隆抗体的杂交瘤，通过使用单克隆抗体的免疫反应检测IFNα8及其突变蛋白的方法来解决上述目的，纯化方法 使用单克隆抗体的IFNα8及其突变蛋白，以及用于治疗其发作或恶化与IFNα8相关的疾病的治疗剂，其含有单克隆抗体作为有效成分。

公开(公告)号：US06713618B1

公开(公告)日：2004-03-30

申请号：US09578921

申请日：2000-05-26

Applicant: Yoshiaki Yanai ,  Harumi Ariyasu ,  Tsunetaka Ohta ,  Masashi Kurimoto

Inventor： Yoshiaki Yanai ,  Harumi Ariyasu ,  Tsunetaka Ohta ,  Masashi Kurimoto

IPC: C07H2104

CPC classification number: C12Y302/01028 ,  A01K2217/05 ,  C12N9/2402

Abstract: Disclosed are a DNA which encodes murine trehalase, a polypeptide expressed by the DNA, and a transgenic- and knockout- animals which have been genetically engineered with the DNA. The DNA comprises a part or the whole of the nucleotide sequence of SEQ ID NO:1.

Abstract translation: 公开了编码鼠海藻糖酶的DNA，由DNA表达的多肽，以及用DNA进行遗传工程改造的转基因和敲除动物。 DNA包含SEQ ID NO：1的核苷酸序列的一部分或全部。

公开(公告)号：US5910303A

公开(公告)日：1999-06-08

申请号：US570944

申请日：1995-12-12

Applicant: Kazue Takayama ,  Akira Harashima ,  Tsunetaka Ohta ,  Noboru Izumi ,  Hiroyoshi Tahata ,  Masashi Kurimoto

Inventor： Kazue Takayama ,  Akira Harashima ,  Tsunetaka Ohta ,  Noboru Izumi ,  Hiroyoshi Tahata ,  Masashi Kurimoto

IPC: A61K38/21 ,  A61K38/19 ,  A61K38/20

CPC classification number: A61K38/217

Abstract: An agent which contains, as effective ingredients, interferon-.gamma. and a biologically active substance with either or both activities of promoting production of platelets and leukocytes. A small amount of the agent effectively promotes platelet and/or leukocyte production in mammals including humans without causing side effects.

Abstract translation: 含有作为有效成分的干扰素-γ和具有促进血小板和白细胞产生活性的生物活性物质的药剂。 少量的药剂有效地促进包括人在内的哺乳动物的血小板和/或白细胞产生而不引起副作用。

公开(公告)号：US5585474A

公开(公告)日：1996-12-17

申请号：US555860

申请日：1995-11-13

Applicant: Kanso Iwaki ,  Tsunetaka Ohta ,  Masahi Kurimoto

Inventor： Kanso Iwaki ,  Tsunetaka Ohta ,  Masahi Kurimoto

IPC: A61K38/00 ,  A61P35/00 ,  A61P35/04 ,  C07K14/47 ,  C12N5/06 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/91 ,  C07H19/00 ,  C07H21/00 ,  C07K1/00 ,  C07K14/00

CPC classification number: C07K14/4703 ,  A61K38/00

Abstract: Disclosed is a novel protein which has a molecular weight of 45,000.+-.5,000 and pI 5.7.+-.0.5 and exhibits cancer metastasis-inhibitory activity. The protein can be prepared by culturing human cells, animal cells and microorganisms capable of producing the protein in a nutrient culture medium while stimulating them with an inducer such as Bacille Calmette-Gu erin and lipopolysaccharide.

Abstract translation: 公开了一种新型蛋白质，其分子量为45,000 +/- 5,000，pI 5.7 +/- 0.5，具有癌转移抑制活性。 蛋白质可以通过在营养培养基中培养能够产生蛋白质的人细胞，动物细胞和微生物，同时用诸如卡介苗和脂多糖的诱导剂刺激它们来制备。