公开(公告)号：US20190119743A1

公开(公告)日：2019-04-25

申请号：US16172310

申请日：2018-10-26

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Zhaohui Sun ,  Shengrong Lin

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12P19/34

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, or two different sheared polynucleotides as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described method.

公开(公告)号：US11578359B2

公开(公告)日：2023-02-14

申请号：US16927898

申请日：2020-07-13

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Shengrong Lin ,  Ling Fung Tang

IPC: C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/68 ,  G16B25/00 ,  G16B25/20

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

公开(公告)号：US10767222B2

公开(公告)日：2020-09-08

申请号：US15102241

申请日：2014-12-11

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Shengrong Lin ,  Zhaohui Sun ,  Grace Qizhi Zhao ,  Paul Ling-Fung Tang

IPC: C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6869

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods.

公开(公告)号：US20180057871A1

公开(公告)日：2018-03-01

申请号：US15800558

申请日：2017-11-01

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Zhaohui Sun ,  Shengrong Lin

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6869 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2521/501 ,  C12Q2531/125 ,  C12Q2535/122

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, or two different sheared polynucleotides as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods.