公开(公告)号：US11859246B2

公开(公告)日：2024-01-02

申请号：US16689018

申请日：2019-11-19

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Shengrong Lin ,  Lin Fung Tang

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6874

CPC classification number: C12Q1/6865 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

公开(公告)号：US11286519B2

公开(公告)日：2022-03-29

申请号：US16434941

申请日：2019-06-07

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Shengrong Lin ,  Lin Fung Tang

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6874

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

公开(公告)号：US10724088B2

公开(公告)日：2020-07-28

申请号：US16172310

申请日：2018-10-26

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Zhaohui Sun ,  Shengrong Lin

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, or two different sheared polynucleotides as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described method.

公开(公告)号：US10155980B2

公开(公告)日：2018-12-18

申请号：US15800558

申请日：2017-11-01

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Zhaohui Sun ,  Shengrong Lin

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, or two different sheared polynucleotides as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods.

公开(公告)号：US20180298434A1

公开(公告)日：2018-10-18

申请号：US15947100

申请日：2018-04-06

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Shengrong Lin ,  Ling Fung Tang

IPC: C12Q1/6853 ,  C12Q1/6858

CPC classification number: C12Q1/6853 ,  C12Q1/68 ,  C12Q1/6858 ,  G16B25/00 ,  C12Q2525/301 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/16 ,  C12Q2525/155

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

公开(公告)号：US12163184B2

公开(公告)日：2024-12-10

申请号：US15780370

申请日：2016-12-02

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Shengrong Lin ,  Malek Faham

IPC: C12P19/34 ,  C12M1/34 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6839 ,  C12Q1/6855

Abstract: In some aspects, the present disclosure provides methods for forming ligation products comprising single-stranded polynucleotides. Ligation products formed by various aspects of the present disclosure can be useful for various applications, including but not limited to sequence analysis. In some embodiments, the ligation products comprise cell-free polynucleotides. In some aspects, the present disclosure provides reaction mixtures, kits and complexes consistent with the methods herein.

公开(公告)号：US11643683B2

公开(公告)日：2023-05-09

申请号：US16896073

申请日：2020-06-08

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Zhaohui Sun ,  Shengrong Lin

IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6858

CPC classification number: C12Q1/6869 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2531/125 ,  C12Q2535/122

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, or two different sheared polynucleotides as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described method.

公开(公告)号：US10752942B2

公开(公告)日：2020-08-25

申请号：US15947100

申请日：2018-04-06

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Li Weng ,  Shengrong Lin ,  Ling Fung Tang

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q1/6858 ,  G16B25/00

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

公开(公告)号：US12234512B2

公开(公告)日：2025-02-25

申请号：US18164439

申请日：2023-02-03

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Shengrong Lin ,  Zhaohui Sun ,  Grace Qizhi Zhao ,  Paul Ling-Fung Tang

IPC: C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6869

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods.

公开(公告)号：US11427866B2

公开(公告)日：2022-08-30

申请号：US16301707

申请日：2017-05-16

Applicant: ACCURAGEN HOLDINGS LIMITED

Inventor： Malek Faham ,  Shengrong Lin ,  Ling Fung Tang ,  Yontao Lu ,  Zhaohui Sun ,  Yingyu Wang ,  Li Weng

IPC: C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858 ,  C40B50/06

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants, as well as methods of determining copy number of a genetic locus in a sample. Systems and kits for performing methods of the disclosure, as well as compositions produced by or useful in methods of the disclosure are also provided. In some embodiments, methods comprise extending 3′ ends of polynucleotides by adding one or more pre-determined nucleotides. In some embodiments, methods comprise use of a strand-tagging sequence.