公开(公告)号：US20240336980A1

公开(公告)日：2024-10-10

申请号：US18747178

申请日：2024-06-18

申请人： Natera, Inc.

发明人： Joshua Babiarz ,  Tudor Pompiliu Constantin ,  Lane A. Eubank ,  George Gemelos ,  Matthew Micah Hill ,  Huseyin Eser Kirkizlar ,  Matthew Rabinowitz ,  Onur Sakarya ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann

IPC分类号： C12Q1/6886 ,  C12Q1/6869 ,  G06N7/01 ,  G06N20/00 ,  G16B15/00 ,  G16B20/00 ,  G16B20/10 ,  G16B20/20 ,  G16B25/00 ,  G16B25/20 ,  G16B40/00 ,  G16B40/20 ,  G16H10/40 ,  G16H50/20 ,  G16Z99/00

CPC分类号： C12Q1/6886 ,  C12Q1/6869 ,  G06N7/01 ,  G06N20/00 ,  G16B15/00 ,  G16B20/00 ,  G16B20/10 ,  G16B20/20 ,  G16B25/00 ,  G16B40/00 ,  G16B40/20 ,  G16H10/40 ,  G16H50/20 ,  G16Z99/00 ,  C12Q2539/10 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/172 ,  G16B25/20

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US20240309464A1

公开(公告)日：2024-09-19

申请号：US18678577

申请日：2024-05-30

申请人： Natera, Inc.

发明人： Joshua BABIARZ ,  Tudor Pompiliu CONSTATIN ,  Lane A. EUBANK ,  George GEMELOS ,  Matthew Micah HILL ,  Huseyin Eser KIRKIZLAR ,  Matthew RABIBOWITZ ,  Onur SAKARYA ,  Styrmir SIGURJONSSON ,  Bernhard ZIMMERMANN

IPC分类号： C12Q1/6886 ,  C12Q1/6869 ,  G06N7/01 ,  G06N20/00 ,  G16B15/00 ,  G16B20/00 ,  G16B20/10 ,  G16B20/20 ,  G16B25/00 ,  G16B25/20 ,  G16B40/00 ,  G16B40/20 ,  G16H10/40 ,  G16H50/20 ,  G16Z99/00

CPC分类号： C12Q1/6886 ,  C12Q1/6869 ,  G06N7/01 ,  G06N20/00 ,  G16B15/00 ,  G16B20/00 ,  G16B20/10 ,  G16B20/20 ,  G16B25/00 ,  G16B40/00 ,  G16B40/20 ,  G16H10/40 ,  G16H50/20 ,  G16Z99/00 ,  C12Q2539/10 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/172 ,  G16B25/20

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US20240285200A1

公开(公告)日：2024-08-29

申请号：US18645106

申请日：2024-04-24

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： Abraham SHAKED ,  Bao-Li CHANG ,  Brendan KEATING ,  Toumy GUETTOUCHE

IPC分类号： A61B5/15 ,  A61B10/00 ,  C12N15/113 ,  C12Q1/6883 ,  G16B25/00 ,  G16B25/10 ,  G16B25/20 ,  G16H50/50

CPC分类号： A61B5/15 ,  A61B10/0038 ,  A61B10/007 ,  C12N15/113 ,  C12Q1/6883 ,  G16B25/00 ,  G16B25/10 ,  G16B25/20 ,  G16H50/50 ,  A61B2010/0061 ,  C12Q2600/158 ,  C12Q2600/178

摘要： The present invention relates to the discovery that the expression levels of some microRNAs (miRNAs) can use a diagnostic signature to predict transplant outcomes in a transplant recipient. Thus, in various embodiments described herein, the methods of the invention relate to methods of diagnosing a transplant subject for acute rejection such as acute cellular rejection (ACR), methods of predicting a subject's risk of having or developing ACR and methods of assessing in a subject the likelihood of a successful or failure minimization of immunosuppression therapy (IST) dosage from standard ranges.

公开(公告)号：US20240218450A1

公开(公告)日：2024-07-04

申请号：US18338974

申请日：2023-06-21

申请人： Board of Regents, The University of Texas System ,  Yale University

发明人： William fraser SYMMANS ,  Bruno SINN ,  Christos HATZIS ,  Chunxiao FU ,  Rosanna LAU

IPC分类号： C12Q1/6886 ,  A61K31/138 ,  A61K31/4196 ,  A61K31/566 ,  A61K45/06 ,  A61P35/04 ,  G16B10/00 ,  G16B25/10 ,  G16B25/20

CPC分类号： C12Q1/6886 ,  A61K31/138 ,  A61K31/4196 ,  A61K31/566 ,  A61K45/06 ,  A61P35/04 ,  G16B10/00 ,  G16B25/10 ,  G16B25/20 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： Provided herein are methods of determining tumoral sensitivity to hormonal (endocrine) therapy based upon an index of estrogen receptor (ER)- and progesterone receptor (PR)-related genes, referred to as the sensitivity to endocrine therapy index (SETER/PR index), and may have additional consideration for the proportion of ER gene (ESR1) RNA transcripts that contain a mutation relative to the value of the SETER/PR index. Further provided are methods of treating breast cancer patients determined to be sensitive to an endocrine therapy by the SETER/PR index.

公开(公告)号：US11961590B2

公开(公告)日：2024-04-16

申请号：US16605298

申请日：2018-04-17

申请人： SEEGENE, INC.

发明人： Mi Hyun Jang

IPC分类号： G01N33/48 ,  C12N15/10 ,  G01N33/50 ,  G06F17/12 ,  G16B25/20 ,  G16B30/00

CPC分类号： G16B25/20 ,  C12N15/1089 ,  G06F17/12 ,  G16B30/00

摘要： The present invention relates to optimization logic for preparing an optimal combination of oligonucleotides hybridized with a plurality of target nucleic acid sequences, in a completely different approach from conventional methods, i.e., empirical and manual methods. In addition, the optimization logic of the present invention may be used to (i) preparing an oligonucleotide combination used to detect a plurality of target nucleic acid sequences with a target coverage of interest, (ii) selecting target nucleic acid sequences to be detected by a multiplex target detection with a highest target coverage by using a limited number of oligonucleotides, and (iii) determining a conserved region in a plurality of target nucleic acid sequences.

公开(公告)号：US11915794B2

公开(公告)日：2024-02-27

申请号：US16280885

申请日：2019-02-20

申请人： Chakrabarti Advanced Technology LLC

发明人： Raj Chakrabarti

IPC分类号： G16B25/20 ,  C12Q1/6851 ,  G16B25/00

CPC分类号： G16B25/20 ,  C12Q1/6851 ,  G16B25/00

摘要： In one aspect, methods are described herein for enhancing one or more nucleic acid interactions. For example, in some embodiments, methods of enhancing one or more steps of polymerase chain reaction (PCR) are described herein. In some embodiments, the optimal temperature cycling protocol for one or more PCR cycles can be determined according to methods described herein.

公开(公告)号：US11898198B2

公开(公告)日：2024-02-13

申请号：US17890208

申请日：2022-08-17

申请人： Illumina, Inc.

发明人： Chen Zhao ,  Kevin Wu ,  Han-Yu Chuang ,  Jennifer Lococo ,  Alex So ,  Dwight Baker ,  Tatjana Singer

IPC分类号： C12P19/34 ,  C12Q1/6855 ,  G16B20/20 ,  G16B35/10 ,  G16B30/10 ,  G16B25/20 ,  G16B40/00 ,  G16B20/00 ,  G16B30/00 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876

CPC分类号： C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B20/00 ,  G16B20/20 ,  G16B25/20 ,  G16B30/00 ,  G16B30/10 ,  G16B35/10 ,  G16B40/00 ,  C12Q2525/191 ,  C12Q2525/197 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2563/179

摘要： The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

公开(公告)号：US20230366022A1

公开(公告)日：2023-11-16

申请号：US18190884

申请日：2023-03-27

申请人： Becton, Dickinson and Company

发明人： Stephen P.A. Fodor ,  Glenn K. Fu

IPC分类号： C12Q1/686 ,  C12N15/10 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6869 ,  G16B25/00 ,  G16B30/00 ,  C12Q1/6809 ,  G16B25/20 ,  C12Q1/6837 ,  C12Q1/6851

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B25/20 ,  G16B30/00 ,  G16B25/10

摘要： Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US11810647B2

公开(公告)日：2023-11-07

申请号：US16280693

申请日：2019-02-20

申请人： Chakrabarti Advanced Technology LLC

发明人： Raj Chakrabarti

IPC分类号： G16B25/20 ,  G16B25/00 ,  C12Q1/6851

CPC分类号： G16B25/20 ,  C12Q1/6851 ,  G16B25/00

摘要： In one aspect, methods are described herein for enhancing one or more nucleic acid interactions. For example, in some embodiments, methods of enhancing one or more steps of polymerase chain reaction (PCR) are described herein. In some embodiments, the optimal temperature cycling protocol for one or more PCR cycles can be determined according to methods described herein.

公开(公告)号：US20230332228A1

公开(公告)日：2023-10-19

申请号：US17998722

申请日：2021-05-11

申请人： BAR ILAN UNIVERSITY

发明人： Ayal HENDEL ,  Zohar YAKHINI ,  Ido AMIT

IPC分类号： C12Q1/6876 ,  G16B30/00 ,  G16B25/20 ,  G16B40/00

CPC分类号： C12Q1/6876 ,  G16B30/00 ,  G16B25/20 ,  G16B40/00 ,  C12Q2600/16 ,  C12Q2600/166

摘要： Novel systems methods and kits are disclosed for determining effects of Nucleic Acid (NA) editing procedures. The technique (methods systems and kits) of the present invention are adapted for receiving sequencing data indicative of pluralities of reads, RTx={riTx} and RMc={rjMc}, of the multiplexed amplifications of each of the edit and control NA collections, originating from a similar source of NAs whereby a certain NA editing procedure was applied to the edit NA collection; and processing the sequencing data, per each particular type of adverse effect of interest to determine a statistical model for classifying whether that type of adverse effect actually occurred due to the NA editing procedure. The technique is suitable for determining occurrences of INDEL types and/or TRANSLOCATION types/species of adverse effect. The technique further enables to statistically quantify the rates of occurrence types of adverse effects which are actually caused by the NA editing procedure, and also to provide statistical confidence intervals for these rates according to a desired statistical confidence level.