公开(公告)号：US20190368982A1

公开(公告)日：2019-12-05

申请号：US15995755

申请日：2018-06-01

Applicant: Agilent Technologies, Inc.

Inventor： Kyle Schleifer ,  Kristin Briana Bernick ,  Adrienne Mccampbell ,  Nicholas M. Sampas ,  Victor Lim

IPC: G01N1/30 ,  G01N1/28 ,  G01N1/06 ,  G01N35/00 ,  G06T7/00

Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.