公开(公告)号：US07928191B2

公开(公告)日：2011-04-19

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/16

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质由10mmol / L至1mol / L的咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US20090042745A1

公开(公告)日：2009-02-12

申请号：US12188508

申请日：2008-08-08

申请人： Koichi MINAMI ,  Yohsuke Takeuchi ,  Hirohiko Tsuzuki

发明人： Koichi MINAMI ,  Yohsuke Takeuchi ,  Hirohiko Tsuzuki

IPC分类号： C40B60/12

CPC分类号： G01N33/54353 ,  G01N33/54393 ,  G01N33/553 ,  G01N2610/00

摘要： A biosensor chip is constituted by: a substrate; a metal film which is physically attached to the substrate; and a self assembled monolayer constituted by alkyl chains, which is physically attached to the metal film. Ligands and at least one functional group, selected from a group consisting of a hydroxyl group, a carboxyl group, an alkoxy group, a methyl group, and hydrogen atoms are physically attached to the self assembled monolayer. The ratio between the total number of alkyl chains physically attached to the metal film and the number of alkyl chains which are covalently bonded with the ligands is within a range of 0.40 to 0.99.

摘要翻译： 生物传感器芯片由以下部分构成：基板; 物理地附接到基底的金属膜; 以及由金属膜物理附着的烷基链构成的自组装单层。 配体和选自羟基，羧基，烷氧基，甲基和氢原子的至少一个官能团物理连接到自组装单层。 与金属膜物理连接的烷基链的总数与与配体共价键合的烷基链的数量之间的比例在0.40至0.99的范围内。

公开(公告)号：US06245476B1

公开(公告)日：2001-06-12

申请号：US09328489

申请日：1999-06-09

申请人： Tomohiro Kodama ,  Yohsuke Takeuchi ,  Kimiatsu Nomura ,  Yoshihiro Jimbo ,  Ken Iwakura

发明人： Tomohiro Kodama ,  Yohsuke Takeuchi ,  Kimiatsu Nomura ,  Yoshihiro Jimbo ,  Ken Iwakura

IPC分类号： G03F7016

CPC分类号： G03C1/52 ,  G03C1/002

摘要： The present invention provides a photo-sensitive and heat-sensitive recording material comprising a substrate supporting thereon a photo-sensitive and heat-sensitive recording layer containing a diazo compound and a coupler, wherein the diazo compound is a compound represented by the following general formula (1) and the diazo compound is enclosed in a microcapsule: wherein, R1 and R2 may be the same or different, and represent an alkyl group having 1 to 5 carbon atoms; R1 and R2 may be bonded to each other to form a ring; R3 and R4 may be the same or different, and represent an alkyl group; and R3 and R4 may be bonded to each other to form a ring. This photo-sensitive and heat-sensitive recording material reveals less change in the base concentration between before and after storage before use.

摘要翻译： 本发明提供了一种感光和热敏记录材料，其包括在其上支撑含有重氮化合物和成色剂的感光和热敏记录层的基底，其中重氮化合物是由以下通式表示的化合物 （1），重氮化合物封闭在微胶囊中：其中，R 1和R 2可以相同或不同，表示碳原子数1〜5的烷基。 R1和R2可以彼此键合形成环; R3和R4可以相同或不同，表示烷基; 并且R 3和R 4可以彼此键合形成环。 这种光敏和热敏记录材料在使用前显示储存前后的碱浓度变化较小。

公开(公告)号：US20100167010A1

公开(公告)日：2010-07-01

申请号：US12294775

申请日：2007-03-27

申请人： Yohsuke Takeuchi

发明人： Yohsuke Takeuchi

IPC分类号： B32B3/30 ,  B32B17/10 ,  B32B27/06 ,  C08F212/08 ,  C08L25/08

CPC分类号： C09D5/00 ,  C08L25/06 ,  C08L33/06 ,  C08L2205/02 ,  C09D125/14 ,  Y10T428/24355 ,  Y10T428/31645 ,  Y10T428/31667

摘要： A planing coat includes a copolymer having a hydrocarbon vinyl monomer as a copolymer component, which is formed on a base material and has a slipping rate of 0.5 cm/second or more, the slipping rate being calculated by adding 50 μl of distilled water to a sample mounted on a horizontal surface with a slant of 15 degree so as to form a droplet, and measuring the period of time of the droplet to travel 90 mm under an environment of 20±5° C. in temperature and 50±10% in relative humidity.

摘要翻译： 刨花涂层包括具有作为共聚物成分的烃类乙烯基单体的共聚物，其形成在基材上并具有0.5cm /秒以上的滑移率，通过向50℃蒸馏水中加入50μl蒸馏水来计算滑移率 样品以15度的倾斜安装在水平表面上，以形成液滴，并且在20±5℃的温度和50±10％的温度下测量液滴行进90mm的时间段 相对湿度。