公开(公告)号：US20100317032A1

公开(公告)日：2010-12-16

申请号：US12796676

申请日：2010-06-09

申请人： Koichi MINAMI ,  Hirohiko TSUZUKI ,  Hiroshi UEDA ,  Masaki IHARA

发明人： Koichi MINAMI ,  Hirohiko TSUZUKI ,  Hiroshi UEDA ,  Masaki IHARA

IPC分类号： G01N33/53

CPC分类号： G01N33/5306

摘要： Provided is a method for detecting an antigen in a sample, the method including: bringing an unlabeled polypeptide and a labeled polypeptide into contact with an antigen in a sample, the unlabeled polypeptide being one of a pair including a VH-region polypeptide and a VL-region polypeptide which are separate and capable of cooperatively recognizing the antigen, and the labeled polypeptide being the other of the pair including the separate VH-region polypeptide and the VL-region polypeptide and being labeled with an environmentally-responsive substance at a site where the environmentally-responsive substance does not inhibit binding of the antigen, and detecting a change in the environmentally-responsive substance caused by a change in the environment around the labeled polypeptide after the contact. Also provided is an antibody fragment polypeptide set including the unlabeled polypeptide and the labeled polypeptide.

摘要翻译： 提供了一种用于检测样品中的抗原的方法，所述方法包括：使未标记的多肽和标记的多肽与样品中的抗原接触，所述未标记的多肽是包含VH区多肽和VL的一对之一 - 区域多肽，其是分离的并且能够协同识别抗原，并且标记的多肽是一对中的另一个，包括单独的VH区多肽和VL区多肽，并且在环境响应物质的标记位置处 环境响应物质不抑制抗原的结合，并且检测由接触后标记的多肽周围的环境变化引起的环境响应物质的变化。 还提供了包含未标记的多肽和标记的多肽的抗体片段多肽组。

公开(公告)号：US20100173326A1

公开(公告)日：2010-07-08

申请号：US12651689

申请日：2010-01-04

申请人： Koichi MINAMI

发明人： Koichi MINAMI

IPC分类号： G01N33/53 ,  B05D1/36 ,  B01J19/00

CPC分类号： G01N33/54373

摘要： A self assembling monolayer formed by the self assembling molecules is caused to bond onto the surface of a base material, and chelators are caused to bond onto the self assembling monolayer, to produce a substrate, in which the chelators are bound to the self assembling monolayer at a density within a range from 0.4/nm2 to 4/nm2. The bonding of the chelators onto the self assembling monolayer being performed within an organic solvent.

摘要翻译： 使由自组装分子形成的自组装单层结合到基材的表面上，并使螯合剂结合到自组装单层上以产生底物，其中螯合剂与自组装单层结合 在0.4 / nm 2至4 / nm 2的范围内的密度。 螯合剂在自组装单层上的键合在有机溶剂中进行。

公开(公告)号：US20090140209A1

公开(公告)日：2009-06-04

申请号：US12276004

申请日：2008-11-21

申请人： Koichi MINAMI ,  Hirohiko TSUZUKI

发明人： Koichi MINAMI ,  Hirohiko TSUZUKI

IPC分类号： C09K3/00

CPC分类号： C07K17/00 ,  G01N21/553

摘要： A complex, which has been formed by at least two pieces of a ligand and a first metal ion, is bound with a carrier. A second metal ion is then added onto the carrier, a new complex being thereby formed. A substance that contains a Group-15 or Group-16 atom, which has metal coordinating capability, is then fixed to the first metal ion and the second metal ion.

摘要翻译： 由至少两个配体和第一金属离子形成的络合物与载体结合。 然后将第二金属离子加入到载体上，从而形成新的络合物。 含有具有金属配位能力的第15族或第16族原子的物质然后固定在第一金属离子和第二金属离子上。

公开(公告)号：US20090062515A1

公开(公告)日：2009-03-05

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/00

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质通过10mmol / L至1mol / L咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US4382597A

公开(公告)日：1983-05-10

申请号：US187567

申请日：1980-09-15

申请人： Koichi Minami

发明人： Koichi Minami

IPC分类号： A63F7/02 ,  A63F7/06 ,  A63F7/00

CPC分类号： A63F7/2409 ,  A63F7/025 ,  A63F7/066 ,  A63F2250/04 ,  A63F2250/0407

摘要： A pinball game is contained within a housing which includes a liquid filled chamber. At least one ball is located within the liquid within the chamber. The ball has a specific gravity heavier than the liquid such that it tends to sink within the liquid but it is light enough to be conveyed upwardly by currents within the liquid. A flipper is located within the chamber and is capable of interacting with the ball to propel it within the confines of the chamber. The flipper is controlled by an actuator member located on the housing and operatively connected to the flipper to move the flipper. At least one goal receptacle is positioned within the chamber and the ball is capable of being located or deposited in the goal receptacle. It is the object to deposit the ball in the goal receptacle by manipulating the ball with the flippers and/or currents of liquid.

摘要翻译： 弹珠游戏包含在包括充满液体的腔室的壳体内。 至少一个球位于腔室内的液体内。 球具有比液体更重的比重，使得其倾向于在液体内沉降，但是其足够轻以通过液体内的电流被向上输送。 导板位于腔室内，能够与球体相互作用，以将其推进腔室的范围内。 导板由位于壳体上的致动器构件控制，并且可操作地连接到导板以移动导板。 至少一个目标容器定位在腔室内，并且球能够定位或沉积在球门座中。 目标是通过用脚蹼和/或液体流动操纵球，将球沉积在球门座中。

公开(公告)号：US09487592B2

公开(公告)日：2016-11-08

申请号：US13125478

申请日：2009-10-23

申请人： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

发明人： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

IPC分类号： C07K16/18 ,  C07K16/40

CPC分类号： C07K16/40 ,  C07K16/18 ,  C07K2317/56 ,  Y10T428/269

摘要： An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein the antibody fragments of each set includes at least two types of separate antibody fragments that are capable of recognizing one type of antigen and that are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

摘要翻译： 抗体片段固定底物包括底物和至少一组抗体片段，其中每组的抗体片段包含能够识别一种类型的抗原并且独立地固定在其上的至少两种类型的分离的抗体片段 位置关系的底物允许一组中的每个抗体片段与相同的抗原结合。

公开(公告)号：US20110100913A1

公开(公告)日：2011-05-05

申请号：US12915973

申请日：2010-10-29

申请人： Koichi MINAMI ,  Morihito IKEDA ,  Hiroshi ARAKATSU

发明人： Koichi MINAMI ,  Morihito IKEDA ,  Hiroshi ARAKATSU

IPC分类号： C02F1/44 ,  B01D61/00 ,  B01D11/04

CPC分类号： C02F1/44 ,  B01D61/00 ,  B01D61/002 ,  B01D61/145 ,  B01D61/243 ,  B01D61/364 ,  B01D61/422 ,  B01D61/58 ,  B01D2311/2626 ,  C02F1/444 ,  C02F1/445 ,  C02F1/4693 ,  C02F2301/08 ,  C02F2303/16

摘要： To provide a water purifier, containing: a diluting unit configured to bring targeted water for purification into contact with an aqueous solution containing a volatile solute and a polymer via a semi-permeable membrane so as to separate water from the targeted water by the semi-permeable membrane, and configured to dilute the aqueous solution with the separated water; a separating unit configured to separate the volatile solute and the polymer from the diluted aqueous solution obtained by the diluting unit so as to obtain purified water; and a dissolving unit configured to return the separated volatile solute by the separating unit to the aqueous solution containing the polymer, and to allow the separated volatile solute to dissolve in the aqueous solution containing the polymer.

摘要翻译： 本发明提供一种净水器，其特征在于，含有：稀释单元，其配置为使净化物与含有挥发性溶质的水溶液和聚合物通过半透膜接触，以通过半透过膜将水与目标水分离， 并且被配置为用分离的水稀释水溶液; 分离单元，被配置为从由稀释单元获得的稀释水溶液中分离挥发性溶质和聚合物以获得纯化水; 以及溶解单元，其被配置为将分离单元中分离的挥发性溶质返回到含有聚合物的水溶液中，并且使分离的挥发性溶质溶解在含有聚合物的水溶液中。

公开(公告)号：US20100290954A1

公开(公告)日：2010-11-18

申请号：US12744311

申请日：2008-11-21

申请人： Koichi Minami ,  Hirohiko Tsuzuki

发明人： Koichi Minami ,  Hirohiko Tsuzuki

IPC分类号： G01N21/64 ,  G01N33/543

CPC分类号： G01N33/542 ,  G01N21/6428

摘要： A first reactant, which is provided with a reaction site for specific binding with an analyte, and a fluorescent label site, and a second reactant, which is provided with a reaction site for specific binding with the analyte, and a fluorescence recognition site for recognizing fluorescence produced by the fluorescent label site of the first reactant, are respectively fixed onto a support such that the first reactant and the second reactant have a positional relationship adapted for the binding with the analyte.

摘要翻译： 提供有用于与分析物特异性结合的反应位点的第一反应物和荧光标记位点，以及第二反应物，其具有用于与分析物特异性结合的反应位点和用于识别的荧光识别位点 由第一反应物的荧光标记位点产生的荧光分别固定在载体上，使得第一反应物和第二反应物具有适于与分析物结合的位置关系。

公开(公告)号：US4480834A

公开(公告)日：1984-11-06

申请号：US433031

申请日：1982-10-06

申请人： Koichi Minami

发明人： Koichi Minami

IPC分类号： A63F7/06 ,  A63F7/20 ,  A63H11/06

CPC分类号： A63F7/0612 ,  A63H11/06 ,  A63F7/249

摘要： A toy has a base with a surface thereon. A portion of the surface is movable with respect to the remainder of the surface. A target is mounted on a pedestal above the surface. A self-propelled object is associated with the surface. The object is of the type having a body section and a pedestal section, with a motor located in the body section which is capable of moving the pedestal section with respect to the body section. The object further includes a hand-shaped member which is capable of locking with a post which projects upwardly from the surface of the base near the movable portion of the surface. A moving member is associated with the movable portion of the surface for moving that portion. The moving member is utilized to move the movable portion of the surface such that when the object is located on that portion as the portion moves then the object is also moved.

摘要翻译： 玩具具有在其上的表面的基座。 表面的一部分相对于表面的其余部分是可移动的。 目标安装在表面上方的基座上。 自走物体与表面相关联。 该物体是具有主体部分和基座部分的类型，其中电动机位于主体部分中，其能够相对于主体部分移动基座部分。 该目的还包括手形构件，该手形构件能够利用从表面的可移动部分附近的基部表面向上突出的柱来锁定。 移动构件与用于移动该部分的表面的可移动部分相关联。 移动构件用于移动表面的可移动部分，使得当物体位于该部分上时，随着部分移动，物体也被移动。

公开(公告)号：US4468033A

公开(公告)日：1984-08-28

申请号：US417330

申请日：1982-09-13

申请人： Koichi Minami ,  Tohru Nishimiya

发明人： Koichi Minami ,  Tohru Nishimiya

IPC分类号： A63F7/04 ,  A63F9/00 ,  A63F9/08

CPC分类号： A63F9/0823 ,  A63F9/0819

摘要： A toy game of skill has a housing and a plurality of members rotatably mounted on the housing such that they are in operative association with one another. One of the members is rotated, and in so rotating that member, the rotation is communicated to the rest of the members to simultaneously rotate them. Located on the members are a plurality of object repositories. A plurality of objects, at least one less than the number of object repositories, are positioned within the object repositories. As the members move with respect to one another, the objects are carried on the object repositories. The objects can be transferred from one repository to an empty repository when two of the members are in a position aligning their respective object repositories with one another. However, the objects are retained in any one object repository whenever the members are in a position with respect to one another such that the object repositories are not aligned.

摘要翻译： 本领域的玩具游戏具有壳体和可旋转地安装在壳体上的多个构件，使得它们彼此可操作地相关联。 其中一个构件被旋转，并且在旋转该构件的同时，旋转与构件的其余部分连通以同时旋转它们。 位于成员上的是多个对象库。 多个对象（至少少于对象存储库的数量）位于对象存储库内。 当成员相对于彼此移动时，对象被承载在对象存储库上。 当两个成员处于将其各自的对象存储库彼此对齐的位置时，可以将这些对象从一个存储库传输到一个空的存储库。 但是，只要成员处于相对于彼此的位置，对象库就不对齐，则对象将保留在任何一个对象存储库中。