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公开(公告)号：US20100317032A1
公开(公告)日：2010-12-16
申请号：US12796676
申请日：2010-06-09
申请人： Koichi MINAMI , Hirohiko TSUZUKI , Hiroshi UEDA , Masaki IHARA
发明人： Koichi MINAMI , Hirohiko TSUZUKI , Hiroshi UEDA , Masaki IHARA
IPC分类号： G01N33/53
CPC分类号： G01N33/5306
摘要： Provided is a method for detecting an antigen in a sample, the method including: bringing an unlabeled polypeptide and a labeled polypeptide into contact with an antigen in a sample, the unlabeled polypeptide being one of a pair including a VH-region polypeptide and a VL-region polypeptide which are separate and capable of cooperatively recognizing the antigen, and the labeled polypeptide being the other of the pair including the separate VH-region polypeptide and the VL-region polypeptide and being labeled with an environmentally-responsive substance at a site where the environmentally-responsive substance does not inhibit binding of the antigen, and detecting a change in the environmentally-responsive substance caused by a change in the environment around the labeled polypeptide after the contact. Also provided is an antibody fragment polypeptide set including the unlabeled polypeptide and the labeled polypeptide.
摘要翻译： 提供了一种用于检测样品中的抗原的方法，所述方法包括：使未标记的多肽和标记的多肽与样品中的抗原接触，所述未标记的多肽是包含VH区多肽和VL的一对之一 - 区域多肽，其是分离的并且能够协同识别抗原，并且标记的多肽是一对中的另一个，包括单独的VH区多肽和VL区多肽，并且在环境响应物质的标记位置处 环境响应物质不抑制抗原的结合，并且检测由接触后标记的多肽周围的环境变化引起的环境响应物质的变化。 还提供了包含未标记的多肽和标记的多肽的抗体片段多肽组。
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公开(公告)号：US20090140209A1
公开(公告)日：2009-06-04
申请号：US12276004
申请日：2008-11-21
申请人： Koichi MINAMI , Hirohiko TSUZUKI
发明人： Koichi MINAMI , Hirohiko TSUZUKI
IPC分类号： C09K3/00
CPC分类号： C07K17/00 , G01N21/553
摘要： A complex, which has been formed by at least two pieces of a ligand and a first metal ion, is bound with a carrier. A second metal ion is then added onto the carrier, a new complex being thereby formed. A substance that contains a Group-15 or Group-16 atom, which has metal coordinating capability, is then fixed to the first metal ion and the second metal ion.
摘要翻译： 由至少两个配体和第一金属离子形成的络合物与载体结合。 然后将第二金属离子加入到载体上，从而形成新的络合物。 含有具有金属配位能力的第15族或第16族原子的物质然后固定在第一金属离子和第二金属离子上。
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公开(公告)号：US07449343B2
公开(公告)日：2008-11-11
申请号：US11218656
申请日：2005-09-06
申请人： Hirohiko Tsuzuki
发明人： Hirohiko Tsuzuki
IPC分类号： G01N33/558 , G01N33/553
CPC分类号： G01N33/54373 , Y10S436/805
摘要： It is an object of the present invention to provide a measurement method which can suppress noise when a specific binding reaction between a physiologically active substance and a test substance is measured using a surface plasmon resonance measurement device. The present invention provides a method for measuring a change in surface plasmon resonance which comprises: using a surface plasmon resonance measurement device comprising a metal film, a light source for generating a light beam, an optical system for allowing the above-described light beam to enter the interface of the metal film so that total reflection conditions can be obtained at the interface thereof and so that various incidence angles can be included, a flow channel system comprising a cell formed on the above-described metal film, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at the above interface, and exchanging the liquid contained in the above flow channel system with another liquid, wherein the liquid in the above-described flow channel system is exchanged with another liquid obtained by eliminating insoluble matters from a measurement solvent, and the change in surface plasmon resonance is then measured in a state where the flow of the liquid has been stopped.
摘要翻译： 本发明的目的是提供一种测量方法，当使用表面等离子体共振测量装置测量生理活性物质和测试物质之间的特异性结合反应时，可以抑制噪声。 本发明提供了一种用于测量表面等离子体共振的变化的方法，包括：使用表面等离子体共振测量装置，其包括金属膜，用于产生光束的光源，用于允许上述光束 进入金属膜的界面，使得能够在其界面获得全反射条件，并且可以包括各种入射角，包括形成在上述金属膜上的单元的流路系统和光检测 用于通过测量在上述界面全反射的光束强度来检测表面等离子体共振的状态的装置，以及将上述流道系统中所含的液体与另一种液体进行交换，其中上述流道系统 与通过从测量溶剂中除去不溶物质而获得的另一种液体以及表面质膜的变化进行交换 然后在液体的流动停止的状态下测量单次共振。
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公开(公告)号：US20060187460A1
公开(公告)日：2006-08-24
申请号：US11354839
申请日：2006-02-16
申请人： Hirohiko Tsuzuki
发明人： Hirohiko Tsuzuki
IPC分类号： G01N21/55
CPC分类号： G01N21/553
摘要： It is an object of the present invention to provide a method for obtaining a chromatogram using the SPR analysis method. The present invention provides a method for measuring the change in the surface plasmon resonance by using a surface plasmon resonance measurement device which comprises a metal film, a light source for generating a light beam, an optical system for allowing such a light beam to enter the interface of the metal film so that total reflection conditions can be obtained at the interface thereof and so that various incidence angles can be included, a flow channel system comprising a cell formed on said metal film, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at said interface, and exchanging a liquid contained in the flow channel system; wherein the measurement is carried out by supplying a liquid at a liquid amount such that the absolute amount of molecules adsorbed on the metal film surface is lower than the saturated adsorption amount of said molecules on said metal film surface.
摘要翻译： 本发明的目的是提供使用SPR分析方法获得色谱图的方法。 本发明提供了一种用于通过使用表面等离子体共振测量装置来测量表面等离子体共振的变化的方法，所述表面等离子体共振测量装置包括金属膜，用于产生光束的光源，用于允许这种光束进入 使得能够在其界面处获得全反射条件，并且可以包括各种入射角，包括形成在所述金属膜上的单元的流路系统和用于检测状态的光检测装置 通过测量在所述界面处全反射的光束的强度，并且更换包含在所述流道系统中的液体的表面等离子体共振; 其中所述测量是通过以液体量供给液体来进行的，以使吸附在所述金属膜表面上的分子的绝对量低于所述金属膜表面上的所述分子的饱和吸附量。
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公开(公告)号：US06274301B1
公开(公告)日：2001-08-14
申请号：US09174011
申请日：1998-10-16
IPC分类号： G03C1498
CPC分类号： G03C1/49863 , G03C1/002 , G03C1/04 , G03C1/34 , G03C1/49845 , G03C2200/22 , G03C2200/36
摘要： A photothermographic recording element having improved image tone and improved tone stability during storage is provided wherein at least one of constituent layers comprises (a) polymeric microparticulates containing a dyestuff, (b) microcapsules containing a dyestuff, and/or (c) an organic or inorganic pigment.
摘要翻译： 提供了具有改善的图像色调并且在存储期间具有改善的色调稳定性的光热记录元件，其中至少一个构成层包含（a）含有染料的聚合物微粒，（b）含有染料的微胶囊和/或（c）有机或 无机颜料。
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公开(公告)号：US6093529A
公开(公告)日：2000-07-25
申请号：US60881
申请日：1998-04-16
CPC分类号： G03C1/49845 , G03C1/49872 , G03C1/49863
摘要： An imaging material is provided in the form of a photothermographic or thermographic image forming layer containing an organic silver salt on one surface of a support and further comprising a polyvalent metal ion and a phthalic acid compound on the image forming layer-bearing surface of the support. The imaging material has improved shelf stability before and after image formation and the ease of handling and produces high-quality blue black tone images.
摘要翻译： 成像材料以支持体的一个表面上含有有机银盐的光热照相或热成像图像形式的形式提供，并且在载体的图像形成层承载表面上还包含多价金属离子和邻苯二甲酸化合物 。 成像材料在图像形成之前和之后具有改善的储存稳定性和易于处理并产生高质量的蓝黑色调图像。
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公开(公告)号：US5922529A
公开(公告)日：1999-07-13
申请号：US996914
申请日：1997-12-23
申请人： Hirohiko Tsuzuki , Hisashi Okada , Yoshio Inagaki
发明人： Hirohiko Tsuzuki , Hisashi Okada , Yoshio Inagaki
CPC分类号： G03C1/49854 , G03C1/49845
摘要： A photothermographic material has a photosensitive layer containing a binder, organic silver salt, reducing agent, and silver halide on one surface of a support. The photosensitive layer further contains a specific sulfur-containing compound and a merocyanine dye of specific structure and has an absorbance of 0.15-1.0 at an exposure wavelength. Sharpness, sensitivity, and aging stability are improved.
摘要翻译： 光热敏成像材料在载体的一个表面上具有含有粘合剂，有机银盐，还原剂和卤化银的感光层。 感光层还含有特定的含硫化合物和特定结构的部花青染料，并且在曝光波长下的吸光度为0.15-1.0。 清晰度，灵敏度和老化稳定性得到提高。
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公开(公告)号：US20110237462A1
公开(公告)日：2011-09-29
申请号：US13132834
申请日：2009-12-04
申请人： Koichi Minami , Hirohiko Tsuzuki , Hiroshi Ueda , Masaki Ihara
发明人： Koichi Minami , Hirohiko Tsuzuki , Hiroshi Ueda , Masaki Ihara
CPC分类号： G01N33/54353
摘要： An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein antibody fragments of each set include at least two types of separate antibody fragments, the at least two types of separate antibody fragments include at least one type of labeled antibody fragment having a labeled site labeled with a luminescent substance and at least one type of acceptor antibody fragment having an acceptor site for accepting emission from the labeled antibody fragment, and the at least one type of labeled antibody fragment and the at least one type of acceptor antibody fragment are capable of cooperatively recognizing one type of antigen in combination and are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.
摘要翻译： 抗体片段固定的底物包括底物和至少一组抗体片段，其中每组的抗体片段包括至少两种类型的分离的抗体片段，所述至少两种类型的分离的抗体片段包括至少一种类型的 具有用发光物质标记的标记位点的标记抗体片段和至少一种具有用于接受来自标记抗体片段的发射的受体位点的受体抗体片段，以及至少一种类型的标记抗体片段和至少一种类型 的受体抗体片段能够组合地协同识别一种类型的抗原，并以允许一组中的每个抗体片段结合相同抗原的位置关系独立地固定在底物上。
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9.发明申请BIOSENSOR CHIP, PROCESS FOR PRODUCING THE SAME, AND SENSOR FOR SURFACE PLASMON RESONANCE ANALYSIS 审中-公开生物传感器芯片，其生产方法和表面等离子体共振分析的传感器公开(公告)号：US20090062146A1
公开(公告)日：2009-03-05
申请号：US12201467
申请日：2008-08-29
CPC分类号： C40B50/18 , B01J19/0046 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00702 , B01J2219/00734 , G01N33/54373 , G01N2405/00
摘要： A biosensor chip includes a substrate, a polymer having an anionic functional group and being arranged on a surface of the substrate, a polyamino group which is directly or indirectly bound to the anionic functional group at a surface of the polymer, and a long-chain alkyl-based group which is directly or indirectly bound to the anionic functional group at the surface of the polymer.
摘要翻译： 生物传感器芯片包括基板，具有阴离子官能团并且布置在基板表面上的聚合物，在聚合物表面直接或间接地结合阴离子官能团的聚氨基，长链 在聚合物表面直接或间接结合阴离子官能团的烷基基团。
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公开(公告)号：US20080274448A1
公开(公告)日：2008-11-06
申请号：US11710429
申请日：2007-02-26
IPC分类号： C12Q1/00
CPC分类号： G01N33/582 , G01N33/542 , G01N2458/30
摘要： An object of the present invention is to provides a method for detecting a hydrophobic region of a target substance that is capable of forming a hydrophobic region, such as a protein, which can detect a fluorescent analyte with high sensitivity with the use of a simple detection apparatus. The present invention provides a method for detecting a hydrophobic region of a target substance which comprises steps of allowing a target substance capable of forming a hydrophobic region to come into contact with a chemiluminescent substance, and assaying chemiluminescence.
摘要翻译： 本发明的目的是提供一种用于检测能够形成疏水性区域的目标物质的疏水性区域的方法，例如蛋白质，其可以通过使用简单检测来检测具有高灵敏度的荧光分析物 仪器。 本发明提供了一种检测目标物质的疏水区域的方法，其包括使能够形成疏水区域的目标物质与化学发光物质接触的步骤，以及测定化学发光的步骤。
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