公开(公告)号：US20090062515A1

公开(公告)日：2009-03-05

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/00

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质通过10mmol / L至1mol / L咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US07928191B2

公开(公告)日：2011-04-19

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/16

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质由10mmol / L至1mol / L的咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US08557194B2

公开(公告)日：2013-10-15

申请号：US13188637

申请日：2011-07-22

申请人： Koichi Minami ,  Yohsuke Takeuchi

发明人： Koichi Minami ,  Yohsuke Takeuchi

IPC分类号： B05D5/00 ,  B05D1/36

CPC分类号： G01N33/54353 ,  C07K1/22 ,  C07K2319/21 ,  G01N33/54386 ,  G01N33/54393

摘要： A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×1016 pieces/mm3 to 3.3×1018 pieces/mm3. The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

摘要翻译： 载体包括已经结合在基板的表面上的基板，聚合物膜和已经与聚合物膜结合的配体。 该配体以1.0×10 16个/ mm 3〜3.3×10 18个/ mm 3的范围内的密度与聚合物膜结合。 通过一种方法制备载体，其包括以下步骤：使聚合物膜结合在基板上，并使配体与聚合物膜结合，使配体与聚合物膜结合的步骤在 有机溶剂。

公开(公告)号：US20090081371A1

公开(公告)日：2009-03-26

申请号：US12212942

申请日：2008-09-18

申请人： Koichi Minami ,  Morihito Ikeda ,  Yohsuke Takeuchi

发明人： Koichi Minami ,  Morihito Ikeda ,  Yohsuke Takeuchi

IPC分类号： B05D3/00 ,  C12M1/34

CPC分类号： G01N33/54353 ,  C07K17/06 ,  C07K17/14 ,  G01N33/54386 ,  G01N33/54393

摘要： A substrate includes: a base material; and chelators which are three dimensionally covalently bound to the base material at a density of 7.8×1015/mm3 or greater and 4.5×1017/mm3 or less, or two dimensionally covalently bound to the base material at a planar density of 2.0×1011/mm2 or greater and 1.0×1013/mm2 or less. Metal ions are coordinately bound to the chelators, and a bioactive substance having histidine tags are coordinately bound to the metal ions. A blocking agent having ligand sets are coordinately bound to the metal ions to which the bioactive substance is not coordinately bound.

摘要翻译： 基板包括：基材; 和以7.8×10 15 / mm 3以上且4.5×10 17 / mm 3以下的密度三维共价结合于基材的螯合剂，或者以2.0×10 11 / mm 2以上的平面密度二维共价结合到基材上 和1.0×10 13 / mm 2以下。 金属离子与螯合剂配位结合，具有组氨酸标签的生物活性物质与金属离子配位结合。 具有配体组的封闭剂与生物活性物质不配位结合的金属离子配位结合。

公开(公告)号：US20090016933A1

公开(公告)日：2009-01-15

申请号：US12171751

申请日：2008-07-11

申请人： Koichi MINAMI ,  Yohsuke Takeuchi

发明人： Koichi MINAMI ,  Yohsuke Takeuchi

IPC分类号： G01N21/01 ,  G01N33/543 ,  B01J19/00

CPC分类号： G01N33/54353 ,  C07K1/22 ,  C07K2319/21 ,  G01N33/54386 ,  G01N33/54393

摘要： A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×1016 pieces/mm3 to 3.3×1018 pieces/mm3. The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

摘要翻译： 载体包括已经结合在基板的表面上的基板，聚合物膜和已经与聚合物膜结合的配体。 该配体以1.0×10 16个/ mm 3至3.3×10 18个/ mm 3的范围内的密度与聚合物膜结合。 通过一种方法制备载体，其包括以下步骤：使聚合物膜结合在基板上，并使配体与聚合物膜结合，使配体与聚合物膜结合的步骤在 有机溶剂。

公开(公告)号：US20110274846A1

公开(公告)日：2011-11-10

申请号：US13188637

申请日：2011-07-22

申请人： Koichi Minami ,  Yohsuke Takeuchi

发明人： Koichi Minami ,  Yohsuke Takeuchi

IPC分类号： B05D5/00 ,  B05D1/36

CPC分类号： G01N33/54353 ,  C07K1/22 ,  C07K2319/21 ,  G01N33/54386 ,  G01N33/54393

摘要： A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×1016 pieces/mm3 to 3.3×1018 pieces/mm3. The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

摘要翻译： 载体包括已经结合在基板的表面上的基板，聚合物膜和已经与聚合物膜结合的配体。 该配体以1.0×10 16个/ mm 3至3.3×10 18个/ mm 3的范围内的密度与聚合物膜结合。 通过一种方法制备载体，其包括以下步骤：使聚合物膜结合在基板上，并使配体与聚合物膜结合，使配体与聚合物膜结合的步骤在 有机溶剂。

公开(公告)号：US20090042745A1

公开(公告)日：2009-02-12

申请号：US12188508

申请日：2008-08-08

申请人： Koichi MINAMI ,  Yohsuke Takeuchi ,  Hirohiko Tsuzuki

发明人： Koichi MINAMI ,  Yohsuke Takeuchi ,  Hirohiko Tsuzuki

IPC分类号： C40B60/12

CPC分类号： G01N33/54353 ,  G01N33/54393 ,  G01N33/553 ,  G01N2610/00

摘要： A biosensor chip is constituted by: a substrate; a metal film which is physically attached to the substrate; and a self assembled monolayer constituted by alkyl chains, which is physically attached to the metal film. Ligands and at least one functional group, selected from a group consisting of a hydroxyl group, a carboxyl group, an alkoxy group, a methyl group, and hydrogen atoms are physically attached to the self assembled monolayer. The ratio between the total number of alkyl chains physically attached to the metal film and the number of alkyl chains which are covalently bonded with the ligands is within a range of 0.40 to 0.99.

摘要翻译： 生物传感器芯片由以下部分构成：基板; 物理地附接到基底的金属膜; 以及由金属膜物理附着的烷基链构成的自组装单层。 配体和选自羟基，羧基，烷氧基，甲基和氢原子的至少一个官能团物理连接到自组装单层。 与金属膜物理连接的烷基链的总数与与配体共价键合的烷基链的数量之间的比例在0.40至0.99的范围内。

公开(公告)号：US07205083B2

公开(公告)日：2007-04-17

申请号：US10690779

申请日：2003-10-23

申请人： Yohsuke Takeuchi ,  Yoshimitsu Arai ,  Naoto Yanagihara

发明人： Yohsuke Takeuchi ,  Yoshimitsu Arai ,  Naoto Yanagihara

IPC分类号： G03F7/021

CPC分类号： B41M5/155 ,  B41M5/124 ,  B41M5/165 ,  B41M5/3333

摘要： A recording material comprises, on a support, a recording layer including a diazo compound, a coupler compound that can react with the diazo compound to form a color, and a metal salt, wherein the coupler compound is represented by the general formula (1):General Formula (1) wherein R1, R2, R3, and R4 each independently represent a hydrogen atom, an alkyl group, an aryl group, an alkoxy group, or an amino group; R5, R6, R7, R8, and R9 each independently represent a hydrogen atom, a halogen atom, an alkyl group, an aryl group, an alkoxy group, an aryloxy group, an alkylthio group, an arylthio group, an alkylsulfonyl group, an arylsulfonyl group, an alkoxycarbonyl group, an aryloxycarbonyl group, an acyloxy group, an acyl group, a carbamoyl group, an acylamino group, a sulfamoyl group, a sulfonamide group, a cyano group, or a nitro group; and X represents an oxygen atom or a sulfur atom.

摘要翻译： 记录材料在载体上包含包含重氮化合物，可与重氮化合物反应形成颜料的成色剂化合物的记录层和金属盐，其中所述成色剂化合物由通式（1）表示， ：

公开(公告)号：US07300738B2

公开(公告)日：2007-11-27

申请号：US10773366

申请日：2004-02-09

申请人： Naoki Saito ,  Tetsunori Matsushita ,  Akinori Fujita ,  Yohsuke Takeuchi ,  Satoshi Higuchi ,  Kimi Ikeda

发明人： Naoki Saito ,  Tetsunori Matsushita ,  Akinori Fujita ,  Yohsuke Takeuchi ,  Satoshi Higuchi ,  Kimi Ikeda

IPC分类号： G03F7/021

CPC分类号： G03C1/54 ,  G03C1/58

摘要： A recording material having, on a support, a recording layer containing an azolinyl acetic acid derivative and a diazo compound. The azolinyl acetic acid derivative is preferably is a compound represented by the following general formula (1): wherein X represents an oxygen atom or a sulfur atom; R11 represents an alkyl group, an aryl group, a heterocyclic group, —OR13 or —NR14R15; R12 represents a substituent; R13 represents an alkyl group, an aryl group or a heterocyclic group; R14 and R15 each independently represents a hydrogen atom, an alkyl group, an aryl group or a heterocyclic group; n represents an integer from 0 to 4; and, when n is an integer of 2 or greater, two or more R12s may be linked with each other to form a ring.

摘要翻译： 一种记录材料，在载体上具有包含偶氮二乙酸乙酯衍生物和重氮化合物的记录层。 噻唑啉基乙酸衍生物优选为由以下通式（1）表示的化合物：其中X表示氧原子或硫原子; R 11表示烷基，芳基，杂环基，-OR 13或-NR 14 R 15， SUP>; R 12表示取代基; R 13表示烷基，芳基或杂环基; R 14和R 15各自独立地表示氢原子，烷基，芳基或杂环基; n表示0〜4的整数， 并且当n为2以上的整数时，可以将两个以上的R 12彼此连接形成环。

公开(公告)号：US06245476B1

公开(公告)日：2001-06-12

申请号：US09328489

申请日：1999-06-09

申请人： Tomohiro Kodama ,  Yohsuke Takeuchi ,  Kimiatsu Nomura ,  Yoshihiro Jimbo ,  Ken Iwakura

发明人： Tomohiro Kodama ,  Yohsuke Takeuchi ,  Kimiatsu Nomura ,  Yoshihiro Jimbo ,  Ken Iwakura

IPC分类号： G03F7016

CPC分类号： G03C1/52 ,  G03C1/002

摘要： The present invention provides a photo-sensitive and heat-sensitive recording material comprising a substrate supporting thereon a photo-sensitive and heat-sensitive recording layer containing a diazo compound and a coupler, wherein the diazo compound is a compound represented by the following general formula (1) and the diazo compound is enclosed in a microcapsule: wherein, R1 and R2 may be the same or different, and represent an alkyl group having 1 to 5 carbon atoms; R1 and R2 may be bonded to each other to form a ring; R3 and R4 may be the same or different, and represent an alkyl group; and R3 and R4 may be bonded to each other to form a ring. This photo-sensitive and heat-sensitive recording material reveals less change in the base concentration between before and after storage before use.

摘要翻译： 本发明提供了一种感光和热敏记录材料，其包括在其上支撑含有重氮化合物和成色剂的感光和热敏记录层的基底，其中重氮化合物是由以下通式表示的化合物 （1），重氮化合物封闭在微胶囊中：其中，R 1和R 2可以相同或不同，表示碳原子数1〜5的烷基。 R1和R2可以彼此键合形成环; R3和R4可以相同或不同，表示烷基; 并且R 3和R 4可以彼此键合形成环。 这种光敏和热敏记录材料在使用前显示储存前后的碱浓度变化较小。