公开(公告)号：US07933539B2

公开(公告)日：2011-04-26

申请号：US12416286

申请日：2009-04-01

申请人： Hiroshi Kubota ,  Koichi Mihara ,  Hisashi Kunihiro

发明人： Hiroshi Kubota ,  Koichi Mihara ,  Hisashi Kunihiro

IPC分类号： G03G15/08

CPC分类号： G03G15/0893 ,  G03G15/0872 ,  G03G15/0877 ,  G03G15/0879 ,  G03G2215/0819 ,  G03G2215/0838

摘要： A toner hopper contains toner supplied from a toner container and supplies the toner to a developing device. The toner hopper includes: a container tank for containing the toner; a carrying screw which is disposed in the container tank and is rotated so as to carry the toner in the container tank; and an elastic member having a first section which is fixed to the container tank and a second section which comes into contact with the carrying screw, the first section being farther from the carrying screw than the second section in a direction perpendicular to an axis line of the carrying screw. According to the arrangement, it is possible to stabilize an amount of toner supplied from the toner hopper to the developing device.

摘要翻译： 墨粉盒包含从调色剂容器供应的调色剂并将调色剂供应到显影装置。 调色剂料斗包括：用于容纳调色剂的容器罐; 一个承载螺丝，它设置在容器罐中并被转动以将调色剂携带在容器箱中; 以及弹性构件，其具有固定到所述容器箱的第一部分和与所述承载螺钉接触的第二部分，所述第一部分在垂直于所述承载螺钉的轴线的方向上比所述第二部分远离所述承载螺钉 携带螺丝。 根据该结构，可以将从调色剂料斗供给的调色剂量稳定到显影装置。

公开(公告)号：US20110067659A1

公开(公告)日：2011-03-24

申请号：US12876722

申请日：2010-09-07

申请人： Masaki SUGIYAMA ,  Toshihiro Tomita ,  Masaki Kurimoto ,  Hiroshi Kubota

发明人： Masaki SUGIYAMA ,  Toshihiro Tomita ,  Masaki Kurimoto ,  Hiroshi Kubota

IPC分类号： F01M9/10

CPC分类号： F01M1/04 ,  F01M9/10 ,  F01M11/067 ,  F01M13/022 ,  F01M13/025 ,  F01M13/0405

摘要： A lubrication system for a portable four-stroke engine includes an opening end of the breather passage is disposed at substantially a center of a valve-operating chamber, and the valve-operating chamber is formed by attaching a valve-operating chamber cover. An inner cover is attached to an inner surface of the valve-operating chamber cover so as to be provided along and in contact with the inner surface of the valve-operating chamber cover. A suction passage is formed as a gap between the circumferential edge of the top plate portion and the inner cover. Three or more suction tubes that are in communication with the suction passage are provided in the inner cover, each of the suction tubes having an opening end. At least one of the opening ends of the suction tubes is provided lower than the opening end of the breather passage in an attitude of the engine during use.

摘要翻译： 用于便携式四冲程发动机的润滑系统包括通气通道的开口端设置在阀操作室的大致中心处，并且阀操作室通过安装阀操作室盖而形成。 内盖附接到阀操作室盖的内表面，以便与阀操作室盖的内表面一并设置。 吸入通道形成为顶板部分的圆周边缘和内盖之间的间隙。 与吸入通道连通的三个或更多个吸管设置在内盖中，每个吸管具有开口端。 吸入管的开口端中的至少一个在使用期间以发动机的姿态设置得比通气通道的开口端低。

公开(公告)号：US20100197015A1

公开(公告)日：2010-08-05

申请号：US12695855

申请日：2010-01-28

申请人： Lola M. Reid ,  Nicholas Moss ,  Hiroshi Kubota

发明人： Lola M. Reid ,  Nicholas Moss ,  Hiroshi Kubota

IPC分类号： C12N5/071

CPC分类号： C12N5/0672 ,  A61K35/12 ,  A61K2039/55594 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2503/02

摘要： Methods of isolating and cryopreserving progenitors from human liver are disclosed which include processing human liver tissue to provide a substantially single cell suspension comprising progenitors and non-progenitors of one or more cell lineages found in human liver; subjecting the suspension to a debulking step, which reduces substantially the number of non-progenitors in the suspension, and which provides a debulked suspension enriched in progenitors exhibiting one or more markers associated with at least one of the one or more cell lineages; and selecting from said debulked suspension those cells, which themselves, their progeny, or more mature forms thereof express one or more markers associated with at least one of the one or more cell lineages. Among these markers are CD14, CD34, CD38, CD45, and ICAM. Hepatic progenitors are characterized as being 6-15μ in diameter, diploid, glycophorin A−, CD45−, AFP+++, ALB+, ICAM+, and with subpopulations varying in expression of CD14+. CD34++, CD38++, CD117+. These progenitor subpopulations have characteristics expected for cells that are particularly useful in liver cell and gene therapies and for establishing bioartificial organs.

摘要翻译： 公开了从人肝脏分离和冷冻保存祖细胞的方法，其包括加工人肝组织以提供包含在人肝脏中发现的一种或多种细胞谱系的祖细胞和非祖细胞的基本上单细胞悬浮液; 使悬浮液经历减压步骤，其减少了悬浮液中的非祖细胞的数量，并且提供了富含前体细胞的减数分裂的悬浮液，其显示出与一种或多种细胞系中的至少一种相关联的一种或多种标志物; 以及从所述减粘悬浮液中选择这些细胞，其本身，其子代或更成熟的形式表达与所述一种或多种细胞谱系中的至少一种相关联的一种或多种标志物。 这些标记物中有CD14，CD34，CD38，CD45和ICAM。 肝祖细胞的特征是直径为6-15μ，二倍体，血型糖蛋白A-，CD45-，AFP +++，ALB +，ICAM +，以及与CD14 +表达不同的亚群。 CD34 ++，CD38 ++，CD117 +。 这些祖细胞亚群具有特别适用于肝细胞和基因疗法以及建立生物人造器官的细胞的特征。

公开(公告)号：US20090238413A1

公开(公告)日：2009-09-24

申请号：US12388820

申请日：2009-02-19

申请人： Naru IKEDA ,  Hiroshi Kubota ,  Yuki Kudo

发明人： Naru IKEDA ,  Hiroshi Kubota ,  Yuki Kudo

IPC分类号： G06K9/00 ,  B05D1/00

CPC分类号： G01N21/554 ,  G01N15/1463

摘要： A method for quantifying metal colloidal particles carrying to-be-detected analytes, includes producing an inspection sample by developing solution containing the metal colloidal particles on a substrate, generating a plurality of image data about an identical visual field by enlarging and capturing the sample, removing a signal component derived from an impurity existing on the substrate from the image data by comparing the plurality of image data, and measuring the metal colloidal particles from the image data from which the signal component derived from the impurity was removed.

摘要翻译： 用于定量携带被检测分析物的金属胶体颗粒的方法包括通过在基板上显影含有金属胶体颗粒的溶液来生产检查样品，通过放大和捕获样品产生关于相同视野的多个图像数据， 通过比较多个图像数据，从图像数据中去除源自存在于基板上的杂质的信号分量，以及从除去来自杂质的信号分量的图像数据中测量金属胶体颗粒。

公开(公告)号：US20090161501A1

公开(公告)日：2009-06-25

申请号：US12255151

申请日：2008-10-21

申请人： Hiroshi Kubota ,  Yoshinori Tazaki ,  Kazumi Sugiyama

发明人： Hiroshi Kubota ,  Yoshinori Tazaki ,  Kazumi Sugiyama

IPC分类号： G11B7/00

CPC分类号： G11B7/08511 ,  G11B7/0956

摘要： According to one embodiment, when a focus servo goes off and the distance between an objective lens and a recording layer of an optical disc does not coincide with a focal length of the objective lens, a control voltage or current corresponding to an optical disc wobble stored in a memory is superimposed on a control voltage or current supplied to a driving mechanism to move an objective lens along an optical axis for re-focusing, and the superimposed voltage or current is supplied to the driving mechanism. Therefore, pull-in of a focus servo for re-focusing is possible in a short time.

摘要翻译： 根据一个实施例，当聚焦伺服断开并且物镜与光盘的记录层之间的距离与物镜的焦距不一致时，存储对应于光盘摆动的控制电压或电流 在存储器中叠加在提供给驱动机构的控制电压或电流上以沿着光轴移动物镜以进行再聚焦，并且叠加的电压或电流被提供给驱动机构。 因此，在短时间内可以进行用于重新聚焦的聚焦伺服的拉入。

公开(公告)号：US07526236B2

公开(公告)日：2009-04-28

申请号：US11385769

申请日：2006-03-22

申请人： Hiroshi Kubota ,  Yasuyuki Ishiguro ,  Koichi Mihara

发明人： Hiroshi Kubota ,  Yasuyuki Ishiguro ,  Koichi Mihara

IPC分类号： G03G15/08 ,  G03G21/12

CPC分类号： G03G21/12

摘要： A waste developer collecting device included in a color tandem image forming apparatus which forms an image using K (black), C (cyan), M (magenta), and Y (yellow) developers includes a collecting container which can be attached to a developing device storing the C developer, a developing device storing the M developer, and a developing device storing the Y developer at the same time and which collects the C, M, and Y developers discharged from the respective developing devices. With this, it is possible to efficiently discharge and collect the developers.

摘要翻译： 使用K（黑色），C（青色），M（品红色）和Y（黄色）显影剂形成图像的彩色串联图像形成装置中包括的废弃显影剂收集装置包括可附接到显影剂 存储C显影剂的装置，存储M显影剂的显影装置和同时存储Y显影剂的显影装置，并收集从各显影装置排出的C，M和Y显影剂。 由此，可以有效地排出和收集开发者。

公开(公告)号：US20090068735A1

公开(公告)日：2009-03-12

申请号：US12265381

申请日：2008-11-05

申请人： Hiroshi Kubota ,  Lola M. Reid

发明人： Hiroshi Kubota ,  Lola M. Reid

IPC分类号： C12N5/06 ,  C12N5/02

CPC分类号： G01N33/5091 ,  C12N5/0672 ,  C12N2501/50 ,  C12N2501/58 ,  C12N2501/599

摘要： A method of obtaining a mixture of cells enriched in hepatic progenitors is developed which comprises methods yielding suspensions of a mixture of cell types, and selecting those cells that are classical MHC class I antigen(s) negative and ICAM-1 antigen positive. The weak or dull expression of nonclassical MHC class I antigen(s) can be used for further enrichment of hepatic progenitors. Furthermore, the progenitors can be selected to have a level of side scatter, a measure of granularity or cytoplasmic droplets, that is higher than that in non-parenchymal cells, such as hemopoietic cells, and lower than that in mature parenchymal cells, such as hepatocytes. Furthermore, the progeny of the isolated progenitors can express alpha-fetoprotein and/or albumin and/or CK19. The hepatic progenitors, so isolated, can grow clonally, that is an entire population of progeny can be derived from one cell. The clones of progenitors have a growth pattern in culture of piled-up aggregates or clusters. These methods of isolating the hepatic progenitors are applicable to any vertebrates including human. The hepatic progenitor cell population is expected to be useful for cell therapies, for bioartificial livers, for gene therapies, for vaccine development, and for myriad toxicological, pharmacological, and pharmaceutical programs and investigations.

摘要翻译： 开发了获得富含肝祖细胞的细胞混合物的方法，其包括产生细胞类型混合物的悬浮液的方法，并选择那些经典的MHC I类抗原和ICAM-1抗原阳性的细胞。 非典型MHC I类抗原的弱或暗的表达可用于进一步富集肝祖细胞。 此外，可以选择祖细胞具有高于非实质细胞例如造血细胞的侧向散射，粒度或细胞质液滴的量级，并且低于成熟实质细胞中的祖细胞，例如 肝细胞。 此外，分离的祖细胞的后代可以表达甲胎蛋白和/或白蛋白和/或CK19。 如此分离的肝祖细胞可以克隆生长，即可以从一个细胞中获得后代的全部群体。 祖先的克隆在堆积聚集体或簇的培养中具有增长模式。 这些分离肝祖细胞的方法适用于包括人在内的任何脊椎动物。 预期肝祖细胞群可用于细胞疗法，生物人工肝，基因治疗，疫苗开发和无数毒理学，药理学和药物程序和调查。

公开(公告)号：US20090051265A1

公开(公告)日：2009-02-26

申请号：US11909496

申请日：2006-03-24

申请人： Kunihiko Ikada ,  Hiroshi Kubota ,  Toshiya Tanaka

发明人： Kunihiko Ikada ,  Hiroshi Kubota ,  Toshiya Tanaka

IPC分类号： H01J63/04

CPC分类号： H05B41/2988 ,  H01J61/30

摘要： A self-ballasted fluorescent lamp includes a luminous tube that is formed in a bent shape with a pair of electrode-side end portions located at a the bottom end of the luminous tube. A cover houses a lighting device for lighting the luminous tube. A base is attached to the bottom end of the cover, and the luminous tube is supported at the top end, of the lamp. The outer diameter of a bulb of the luminous tube 14 ranges from 3 to 8 mm, and the maximum width of the luminous tube, is not greater than 30 mm. The cover is formed so that the proportion of the distance by which the cover extends from the base to the lamp length excluding the base ranges from 0 to 25%, and so that the maximum outer diameter of the cover ranges from 1.0 to 1.5 times the outer diameter of the base.

摘要翻译： 自镇流荧光灯包括形成为弯曲形状的发光管，其中一对电极侧端部位于发光管的底端。 盖子包含用于点亮发光管的照明装置。 基座安装在盖的底端，发光管支撑在灯的顶端。 发光管14的灯泡的外径为3〜8mm，发光管的最大宽度为30mm以下。 盖子形成为使得盖子从基座延伸到除了基座之外的灯长度的距离的比例在0至25％的范围内，并且使得盖的最大外径为1.0至1.5倍 底座的外径。

公开(公告)号：US20080298846A1

公开(公告)日：2008-12-04

申请号：US12130734

申请日：2008-05-30

申请人： Toshihide Ohgoshi ,  Koichi Mihara ,  Hideaki Kurimoto ,  Hiroshi Kubota

发明人： Toshihide Ohgoshi ,  Koichi Mihara ,  Hideaki Kurimoto ,  Hiroshi Kubota

IPC分类号： G03G15/08

CPC分类号： G03G15/0872 ,  G03G2215/0668

摘要： A toner bottle for containing toner has a cylindrical body and a scraper. The bottle body can be rotated on its axis by a driving source. The scraper is supported movably in the bottle body. The bottle body has an opening and ribs. The toner in the toner bottle is supplied through the opening to a developing unit. When the bottle body rotates, the ribs convey the toner toward the opening. The scraper includes a shaft and a scraping part. The shaft extends over the whole length of the bottle body and supports the scraping part. A portion of the scraping part is in contact with the inner cylindrical surface of the bottle body. When the bottle body rotates, the scraper moves perpendicularly to the axis of the body.

摘要翻译： 用于容纳调色剂的调色剂瓶具有圆筒体和刮刀。 瓶体可以通过驱动源在其轴上旋转。 刮刀可在瓶体中可移动地支撑。 瓶身有开口和肋骨。 调色剂瓶中的调色剂通过开口供应到显影单元。 当瓶体旋转时，肋向着开口传送调色剂。 刮刀包括轴和刮削部。 轴在瓶体的整个长度上延伸并支撑刮削部分。 刮擦部分的一部分与瓶体的内圆柱表面接触。 当瓶体旋转时，刮刀垂直于身体的轴线移动。

公开(公告)号：US07456017B2

公开(公告)日：2008-11-25

申请号：US10135700

申请日：2002-05-01

申请人： Hiroshi Kubota ,  Lola M. Reid

发明人： Hiroshi Kubota ,  Lola M. Reid

IPC分类号： C12N5/06 ,  C12N5/08

CPC分类号： C12N5/0672 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2502/13

摘要： A method of propagating mammalian endodermally derived progenitors such as hepatic progenitors, their progeny, or mixtures thereof is developed which includes culturing mammalian progenitors, their progeny, or mixtures thereof on a layer of embryonic mammalian feeder cells in a culture medium. The culture medium can be supplemented with one or more hormones and other growth agents. These hormones and other growth agents can include insulin, dexamethasone, transferrin, nicotinamide, serum albumin, β-mercaptoethanol, free fatty acid, glutamine, CuSO4, and H2SeO3. The culture medium can also include antibiotics. Importantly, the culture medium does not include serum.The invention includes means of inducing the differentiation of the progenitors to their adult fates such as the differentiation of hepatic progenitor cells to hepatocytes or biliary cells by adding, or excluding epidermal growth factor, respectively.The method of producing mammalian progenitors is useful in that the progenitors can be used subsequently in one or more of the following processes: identification of growth and differentiation factors, toxicological studies, drug development, antimicrobial studies, or the preparation of an extracorporeal organ such as a bioartificial liver.

摘要翻译： 开发了传播哺乳动物内皮祖细胞如肝祖细胞，其后代或其混合物的方法，其包括在培养基中的胚胎哺乳动物饲养细胞层上培养哺乳动物祖细胞，其后代或其混合物。 培养基可以补充一种或多种激素和其它生长剂。 这些激素和其它生长剂可包括胰岛素，地塞米松，转铁蛋白，烟酰胺，血清白蛋白，β-巯基乙醇，游离脂肪酸，谷氨酰胺，CuSO 4和H 2 SeO 3 。 培养基也可以包括抗生素。 重要的是，培养基不包括血清。 本发明包括诱导祖细胞分化成其成体的方法，例如通过分别添加或排除表皮生长因子将肝祖细胞分化为肝细胞或胆汁细胞。 生产哺乳动物祖细胞的方法是有用的，因为可以随后在以下一个或多个方法中使用祖细胞：识别生长和分化因子，毒理学研究，药物开发，抗微生物研究或体外器官的制备，例如 生物人造肝。