公开(公告)号：US12098391B2

公开(公告)日：2024-09-24

申请号：US16360228

申请日：2019-03-21

Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE

Inventor： Sanjeev Gupta ,  Sriram Bandi

IPC: C12N5/071 ,  A61K35/407

CPC classification number: C12N5/067 ,  A61K35/407 ,  C12N5/0672 ,  C12N2500/84 ,  C12N2501/999 ,  C12N2502/14 ,  C12N2506/02

Abstract: Methods are provided for producing differentiated cells from stem cells, including producing hepatocytes. Compositions thereof are also provided, as are methods of treating a liver disorder.

公开(公告)号：US20240124836A1

公开(公告)日：2024-04-18

申请号：US18391763

申请日：2023-12-21

Applicant: KEIO UNIVERSITY

Inventor： Minoru Ko

IPC: C12N5/0793 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/0797 ,  C12N5/16

CPC classification number: C12N5/0619 ,  C12N5/0606 ,  C12N5/0623 ,  C12N5/067 ,  C12N5/0672 ,  C12N5/16 ,  C12N2501/60 ,  C12N2501/65 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00

Abstract: Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.

公开(公告)号：US11795436B2

公开(公告)日：2023-10-24

申请号：US16865820

申请日：2020-05-04

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  NATIONAL UNIVERSITY OF SINGAPORE

Inventor： Yuanyu Hu ,  Huck Hui Ng ,  Yock Young Dan

IPC: C12N5/071 ,  A61K35/407 ,  C12N5/00 ,  A61P1/16

CPC classification number: C12N5/0672 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0037 ,  C12N5/067 ,  C12N5/0679 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/395 ,  C12N2501/415 ,  C12N2501/42

Abstract: This application describes liver stem cells (LSC), and differentiated hepatocytes, cholangiocytes, and 3D cellular structures derived therefrom. Methods for producing LSC and mature, differentiated hepatocytes and cholangiocytes in culture are provided. Also provided are cell culture systems and cell culture media for producing a homogenous population of liver stem cells that remain in an undifferentiated state over multiple passages in culture. The LSC and methods are useful for producing homogenous populations of hepatocytes and cholangiocytes for downstream applications. The LSC can be transplanted into subjects to treat liver diseases.

公开(公告)号：US20230272347A1

公开(公告)日：2023-08-31

申请号：US18100195

申请日：2023-01-23

Applicant: Koninklijke Nederlandse Akademie Van Wetenschappen

Inventor： Johannes Carolus Clevers ,  Helmuth Gehart

IPC: C12N5/071 ,  A61K35/407 ,  G01N33/50

CPC classification number: C12N5/0672 ,  A61K35/407 ,  C12N5/0671 ,  G01N33/5088 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/345 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/805 ,  C12N2501/999 ,  C12N2506/14

Abstract: The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.

公开(公告)号：US20190194607A1

公开(公告)日：2019-06-27

申请号：US13982661

申请日：2012-01-30

Applicant: Akira Yuo ,  Kumiko Saeki ,  Naoko Nakamura ,  Satoko Matsuyama ,  Miwako Nishio ,  Koichi Saeki ,  Mamoru Hasegawa

Inventor： Akira Yuo ,  Kumiko Saeki ,  Naoko Nakamura ,  Satoko Matsuyama ,  Miwako Nishio ,  Koichi Saeki ,  Mamoru Hasegawa

IPC: C12N5/071 ,  C12Q1/02

CPC classification number: C12N5/067 ,  C12N5/0672 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/39 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45 ,  C12Q1/025 ,  G01N33/5014 ,  G01N33/5067

Abstract: The problem is to produce functional liver cells usable in testing the metabolism and toxicity of a drug, from pluripotent stem cells. The solution includes a method for producing highly functional liver cells using pluripotent stem cells, comprising, from pluripotent stem cells, acquiring primitive endoderm derived from the pluripotent stem cells by a process involving steps (A) and (B), from the primitive endoderm, acquiring liver precursor cells by a process involving step (C), and, from the liver precursor cells, acquiring the highly functional liver cells by a process involving step (D): (A) culturing under serum-free and feeder-free conditions; (B) culturing in the presence of albumin and at least one kind of cytokine; (C) culturing in the presence of SHH or an SHH agonist and at least one kind of cytokine; and (D) culturing and maturing in the presence of at least one kind of cytokine.

公开(公告)号：US10072249B2

公开(公告)日：2018-09-11

申请号：US15026462

申请日：2014-10-16

Applicant: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY

Inventor： Ryuichi Nishinakamura ,  Atsuhiro Taguchi

IPC: C12N5/00 ,  A01N61/00 ,  C07K1/00 ,  C07K14/50 ,  C07K14/51 ,  C12N5/071

CPC classification number: C12N5/0671 ,  C12N5/0672 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45

Abstract: The purpose of the present invention is to provide a process or method that can be utilized when deriving a three-dimensional structure of a kidney from pluripotent stem cells such as ES cells or iPS cells. The differentiation inducing method is characterized by culturing pluripotent cells with the following three steps, in order: (a) a step of culturing an embryoid body induced from the pluripotent stem cells in medium containing Bmp4 and a high-concentration (concentration A) Wnt agonist; (b) a step of culturing the embryoid body in medium which contains activin, Bmp4, retinoic acid and a middle-concentration (concentration B) Wnt agonist; and (c) a step of culturing the embryoid body in medium containing Fgf9 and a low-concentration (concentration C) Wnt agonist (herein, the Wnt agonist concentrations is concentration A>concentration B>concentration C).

公开(公告)号：US10000740B2

公开(公告)日：2018-06-19

申请号：US15028085

申请日：2014-10-06

Applicant: Cambridge Enterprise Limited

Inventor： Ludovic Vallier ,  Nicholas Hannan

IPC: C12N5/071 ,  G01N33/50

CPC classification number: C12N5/068 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/0689 ,  C12N2500/92 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5023

Abstract: This invention relates to the differentiation of pluripotent cells (PSCs) into foregut stem cells (FSCs) using a definitive endoderm induction medium comprising a TGFfi ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor to differentiate the pluripotent cells into definitive endoderm cells and a foregut induction medium comprising a TGFβ ligand to differentiate the definitive endoderm cells into foregut stem cells (FSCs). Methods of differentiation, populations of foregut stem cells, culture media and kits are provided.

公开(公告)号：US20180163179A1

公开(公告)日：2018-06-14

申请号：US15123027

申请日：2015-03-17

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Won-il JEONG ,  Yong-Mahn HAN ,  Mi Young KIM ,  Hyuk Soo EUN ,  Mi-Jin JANG

IPC: C12N5/071 ,  C12Q1/32 ,  C12Q1/30 ,  C12Q1/26

CPC classification number: C12N5/0672 ,  C12N5/067 ,  C12N2500/38 ,  C12N2501/12 ,  C12Q1/02 ,  C12Q1/26 ,  C12Q1/30 ,  C12Q1/32 ,  G01N33/50

Abstract: The present invention relates to a method for improving drug metabolism function of human stem cell-derived hepatocytes. More precisely, the human stem cell-derived hepatocytes are similar in cell morphology to human hepatocytes but display reduced expressions of drug or alcohol metabolism associated enzymes and antioxidant enzymes. So, the inventors co-cultured the hepatocytes differentiated from human stem cells with mouse hepatic stellate cells. As a result, it was confirmed that alcohol mediated toxicity was reduced so that liver cell damage or apoptosis level was reduced. In the meantime, the expressions of drug and alcohol metabolism associated enzymes and antioxidant enzymes were increased.

公开(公告)号：US20170266660A1

公开(公告)日：2017-09-21

申请号：US15509227

申请日：2015-09-30

Applicant: THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

Inventor： Raymond Manohar ANCHAN ,  Sinan GUVEN ,  Utkan DEMIRCI ,  George Luther MUTTER

IPC: B01L3/00 ,  G01N1/42 ,  A61K35/12 ,  C12N5/073 ,  C12N5/071 ,  A01N1/02 ,  C12N1/04

CPC classification number: B01L3/5027 ,  A01N1/0221 ,  A01N1/0268 ,  A01N1/0284 ,  A61K35/12 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1894 ,  C12N1/04 ,  C12N5/0603 ,  C12N5/0672 ,  G01N1/42

Abstract: The technology described herein is directed to methods of cryopreservation, e.g., cryopreservation in a microfluidics format and methods of utilizing cells preserved by such methods.

公开(公告)号：US20170240863A1

公开(公告)日：2017-08-24

申请号：US15315032

申请日：2015-08-28

Applicant: Promethera Biosciences S.A./N.V.

Inventor： Etienne SOKAL ,  Sarah SNYKERS ,  Tuba BARAN ,  Kris GELLYNCK ,  Luca FALCIOLA

IPC: C12N5/071 ,  A61K35/407

CPC classification number: C12N5/0672 ,  A61K35/407 ,  C12N2501/11 ,  C12N2501/12 ,  C12N2501/734 ,  C12N2533/54

Abstract: Novel adult liver progenitor cells (called H2Stem Cells) have been have been characterized on the basis of a series of biological activities and markers. Methods for producing H2Stem Cells allow providing such cells in the form of adherent cells and three-dimensional cell clusters in suspension that can be differentiated into cells having strong liver-specific activities and/or that can be used for treating liver diseases or for evaluating the efficacy, the metabolism, and/or toxicity of a compound.