公开(公告)号：US12049632B2

公开(公告)日：2024-07-30

申请号：US17339112

申请日：2021-06-04

Applicant: BIOCON LIMITED

Inventor： Nagaraj Govindappa ,  Kedarnath Sastry ,  Maria Melina Soares

IPC: C07K14/705 ,  A61K38/00 ,  A61K38/10 ,  A61K38/17 ,  A61K39/00 ,  A61K39/395 ,  A61K45/06 ,  A61K48/00 ,  C07K7/08 ,  C07K14/00 ,  C07K14/495 ,  C07K14/65 ,  C07K14/71 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  C12N5/00 ,  C12N15/62 ,  G01N33/68

CPC classification number: C12N15/62 ,  A61K38/10 ,  A61K38/179 ,  A61K39/395 ,  A61K39/39558 ,  A61K45/06 ,  A61K48/005 ,  C07K7/08 ,  C07K14/00 ,  C07K14/495 ,  C07K14/65 ,  C07K14/70532 ,  C07K14/70596 ,  C07K14/71 ,  C07K16/2818 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/30 ,  C07K16/32 ,  C12N5/0018 ,  G01N33/6854 ,  A61K38/00 ,  A61K2039/505 ,  A61K2121/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/732 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/33 ,  C12N2500/22

Abstract: The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.

公开(公告)号：US20240076612A1

公开(公告)日：2024-03-07

申请号：US18133237

申请日：2023-04-11

Applicant: SALK INSTITUTE FOR BIOLOGICAL STUDIES

Inventor： Fred H. Gage ,  Cedric Bardy

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/13 ,  C12N2501/39

Abstract: Provided herein are, inter alia, are media compositions useful for culturing neural cells. In particular, the compositions provided herein mimic important physiological conditions in the living brain and sustain neural activity. The media compositions provided herein improve the efficiency of human neuron maturation and promote synaptic function in long-term in vitro cultures.

公开(公告)号：US20190240142A1

公开(公告)日：2019-08-08

申请号：US16389572

申请日：2019-04-19

Applicant: Dong Hyun AHN

Inventor： Dong Hyun AHN

IPC: A61K8/98 ,  A61K38/18 ,  A61K8/64 ,  A61K31/164 ,  A61K33/30 ,  A61K35/12 ,  A61K8/27 ,  A61Q7/00 ,  A61K8/67 ,  C12N5/077 ,  A61K8/42 ,  A61K31/4188

CPC classification number: A61K8/981 ,  A61K8/27 ,  A61K8/42 ,  A61K8/64 ,  A61K8/673 ,  A61K31/164 ,  A61K31/4188 ,  A61K33/30 ,  A61K35/12 ,  A61K35/28 ,  A61K38/18 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/1866 ,  A61K2800/78 ,  A61K2800/85 ,  A61Q7/00 ,  C12N5/0653 ,  C12N5/0663 ,  C12N2500/22 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80 ,  A61K2300/00

Abstract: Provided is a hair growth-promoting composition comprising: 100 parts by weight of a stem cell culture solution obtained from a culture medium of mesenchymal stem cells which have been subcultured two or more times; 0.001 to 0.1 parts by weight of zinc; 5 to 50 parts by weight of a panthenol based compound; and 0.0001 to 0.1 parts by weight of a water-soluble vitamin, wherein it is sprayed or applied on the scalp to promote hair growth. Said hair growth-promoting composition may have an excellent effect and improve economical efficiency in treating alopecia due to a simple delivery method of the composition.

公开(公告)号：US20180094240A1

公开(公告)日：2018-04-05

申请号：US15726672

申请日：2017-10-06

Applicant: Kiho Lee ,  Randall S. Prather

Inventor： Kiho Lee ,  Randall S. Prather

IPC: C12N5/075 ,  C12N15/877 ,  A01K67/027

CPC classification number: C12N5/0609 ,  A01K67/0273 ,  A01K2227/108 ,  C12N15/877 ,  C12N15/8778 ,  C12N2500/14 ,  C12N2500/22 ,  C12N2501/405 ,  C12N2517/04 ,  C12N2517/10

Abstract: The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca2+ in the oocyte prior to reduction of the intracellular Zn2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.

公开(公告)号：US20180072986A1

公开(公告)日：2018-03-15

申请号：US15560032

申请日：2016-03-07

Applicant: Hong Woo PARK

Inventor： Hong Woo PARK ,  Bong Gyun KIM

IPC: C12N5/00 ,  C12P21/00 ,  C07K16/00

CPC classification number: C12N5/005 ,  C07K16/00 ,  C07K2317/14 ,  C12N2500/22 ,  C12P21/00 ,  C12P21/02

Abstract: The present invention relates to a cell culture medium using mammalian cells for producing a target material at high efficiency in the mammalian cells, a cell culturing method using same, and a method of producing the target material and, more particularly, to a cell culture medium including Zn ions at a concentration of 30 μM or more in a culture, a salt thereof, or a chelate compound, a cell culturing method using same, and a method of producing a target material. According to the present invention, a cell culture medium for producing recombinant protein by using mammalian cells can be provided, which can achieve excellent effects in increasing antibody production, without showing any cell growth inhibiting effects.

公开(公告)号：US09765302B2

公开(公告)日：2017-09-19

申请号：US15187345

申请日：2016-06-20

Applicant: REGENERATIVE MEDICAL SOLUTIONS, INC.

Inventor： Xiaofang Xu ,  Jon Odorico ,  Erik Forsberg ,  Amber A. Mael

IPC: G01N33/50 ,  C12N5/071 ,  A61K35/39 ,  A61K9/16 ,  A61K45/06 ,  A61K9/50 ,  A61K35/545

CPC classification number: C12N5/0676 ,  A61K9/1652 ,  A61K9/5036 ,  A61K35/39 ,  A61K35/545 ,  A61K45/06 ,  C12N2500/05 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/507

Abstract: Methods, kits, compositions, and systems are provided for culturing pluripotent stem cells to produce populations of cells comprising beta-like cells (e.g., pancreatic lineage, glucose-responsive, and/or insulin-producing). In particular, culture conditions are provided that result in the generation of beta-like cells from a starting culture of human pluripotent stem cells.

公开(公告)号：US09758582B2

公开(公告)日：2017-09-12

申请号：US15145135

申请日：2016-05-03

Applicant: BIOCON LIMITED

Inventor： Nagaraj Govindappa ,  Kedarnath Sastry ,  Maria Melina Soares

IPC: C07K16/28 ,  A61K38/10 ,  A61K38/18 ,  A61K39/395 ,  C07K14/495 ,  C07K14/00 ,  C07K14/65 ,  C07K14/705 ,  C07K16/32 ,  G01N33/68 ,  A61K38/17 ,  A61K45/06 ,  C07K7/08 ,  C07K14/71 ,  C07K16/30 ,  A61K39/00 ,  A61K38/00

CPC classification number: C12N15/62 ,  A61K38/00 ,  A61K38/10 ,  A61K38/179 ,  A61K39/395 ,  A61K39/39558 ,  A61K45/06 ,  A61K48/005 ,  A61K2039/505 ,  A61K2121/00 ,  C07K7/08 ,  C07K14/00 ,  C07K14/495 ,  C07K14/65 ,  C07K14/70532 ,  C07K14/70596 ,  C07K14/71 ,  C07K16/2818 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/30 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/732 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/33 ,  C12N5/0018 ,  C12N2500/22 ,  G01N33/6854

Abstract: The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.

公开(公告)号：US20160319239A1

公开(公告)日：2016-11-03

申请号：US15147541

申请日：2016-05-05

Applicant: Whitehead Institute for Biomedical Research ,  The Brigham and Women's Hospital, Inc.

Inventor： Tan A. Ince

IPC: C12N5/00 ,  C12N5/071 ,  C12N5/09 ,  G01N33/50

CPC classification number: C12N5/0018 ,  C12N5/0682 ,  C12N5/0693 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/46 ,  C12N2500/50 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  G01N33/5008 ,  G01N33/5011

Abstract: Described herein are cell culture media, kits and methods for preparing cell culture media, and methods for culturing cells, for example, cells of the female reproductive tract, and tumor cells.

Abstract translation: 本文描述了用于制备细胞培养基的细胞培养基，试剂盒和方法，以及培养细胞例如雌性生殖道细胞和肿瘤细胞的方法。

公开(公告)号：US20160298088A1

公开(公告)日：2016-10-13

申请号：US15187345

申请日：2016-06-20

Applicant: REGENERATIVE MEDICAL SOLUTIONS, INC.

Inventor： Xiaofang Xu ,  Jon Odorico ,  Erik Forsberg ,  Amber A. Mael

IPC: C12N5/071 ,  G01N33/50 ,  A61K35/39

CPC classification number: C12N5/0676 ,  A61K9/1652 ,  A61K9/5036 ,  A61K35/39 ,  A61K35/545 ,  A61K45/06 ,  C12N2500/05 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/507

Abstract: Methods, kits, compositions, and systems are provided for culturing pluripotent stem cells to produce populations of cells comprising beta-like cells (e.g., pancreatic lineage, glucose-responsive, and/or insulin-producing). In particular, culture conditions are provided that result in the generation of beta-like cells from a starting culture of human pluripotent stem cells.

公开(公告)号：US09441216B2

公开(公告)日：2016-09-13

申请号：US14807745

申请日：2015-07-23

Applicant: Baxalta Incorporated ,  Baxalta GmbH

Inventor： Leopold Grillberger ,  Alexandra Spenger ,  Meinhard Hasslacher ,  Rana Grillberger ,  Manfred Reiter

IPC: C12N9/64

CPC classification number: C12N9/6489 ,  C12N5/0682 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/22 ,  C12N2500/38 ,  C12N2500/95 ,  C12N2500/99 ,  C12N2510/02 ,  C12Y304/24087

Abstract: The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.