公开(公告)号：US07378234B2

公开(公告)日：2008-05-27

申请号：US10512885

申请日：2003-08-27

申请人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Alexander Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

发明人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Alexander Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

IPC分类号： A61K39/00 ,  A61K51/00 ,  A61K39/12 ,  A61K39/29 ,  C12Q1/70

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: —providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, —contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, —detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterizing the HCV-peptide from said complex.

摘要翻译： 描述了分离具有与MHC / HLA分子的结合能力的丙型肝炎病毒肽（HP）或包含所述HCV-肽和所述MHC / HLA分子的复合物的方法，其特征在于以下步骤： - 提供HCV池 肽，所述池包含结合所述MHC / HLA分子的HCV-肽和不结合所述MHC / HLA分子的HCV-肽， - 用所述HCV-肽池接触所述MHC / HLA分子，由此HCV- 具有与所述MHC / HLA分子的结合能力的肽与所述MHC / HLA分子结合，形成包含所述HCV-肽和所述MHC / HLA分子的复合物， - 检测并任选地将所述复合物与所述HCV-肽分离 不结合所述MHC / HLA分子并任选地从所述复合物中分离和表征HCV-肽。

公开(公告)号：US20050221381A1

公开(公告)日：2005-10-06

申请号：US10505929

申请日：2003-02-27

申请人： Christof Klade ,  Juliane Schalich ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Max Birnstiel ,  Gerald Aichinger ,  Alexander Otava ,  Frank Mattner

发明人： Christof Klade ,  Juliane Schalich ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Max Birnstiel ,  Gerald Aichinger ,  Alexander Otava ,  Frank Mattner

IPC分类号： C12Q1/02 ,  A61K35/12 ,  A61K38/00 ,  A61K39/00 ,  A61K39/245 ,  A61P31/12 ,  A61P43/00 ,  C07K7/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/045 ,  C07K16/28 ,  C07K14/74 ,  G01N33/53

CPC分类号： C07K7/06 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C12N2710/16122 ,  G01N2500/04 ,  Y02A50/412

摘要： Described is a method for isolating ligands which have a binding capacity to a MHC/HLA molecule or a complex comprising said ligand and said MHC/HLA molecule which method comprises the following steps:—providing a pool of ligands, said pool containing ligands which bind to said MHC/HLA molecule and ligands which do not bind to said MHC/HLA molecule,—contacting said MHC/HLA molecule with said pool of ligands whereby a ligand which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said ligand and said MHC/HLA molecule is formed,—detecting and optionally separating said complex from the ligands which do not bind to said MHC/HLA molecule and—optionally isolating and characterising the ligand from said complex as well as a method for isolating T cell epitopes which have a binding capacity to a MHC/HLA molecule.

摘要翻译： 描述了分离对MHC / HLA分子具有结合能力的配体或包含所述配体和所述MHC / HLA分子的复合物的方法，该方法包括以下步骤： - 提供配体池，所述池含有结合的配体 对所述MHC / HLA分子和不结合所述MHC / HLA分子的配体， - 用所述配体池接触所述MHC / HLA分子，由此具有与所述MHC / HLA分子的结合能力的配体结合所述MHC / 形成HLA分子和包含所述配体和所述MHC / HLA分子的复合物， - 检测并任选地将所述复合物与不结合所述MHC / HLA分子的配体分离，并任选地从所述复合物中分离和表征配体 作为分离对MHC / HLA分子具有结合能力的T细胞表位的方法。