公开(公告)号：US07378234B2

公开(公告)日：2008-05-27

申请号：US10512885

申请日：2003-08-27

申请人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Alexander Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

发明人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Alexander Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

IPC分类号： A61K39/00 ,  A61K51/00 ,  A61K39/12 ,  A61K39/29 ,  C12Q1/70

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: —providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, —contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, —detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterizing the HCV-peptide from said complex.

摘要翻译： 描述了分离具有与MHC / HLA分子的结合能力的丙型肝炎病毒肽（HP）或包含所述HCV-肽和所述MHC / HLA分子的复合物的方法，其特征在于以下步骤： - 提供HCV池 肽，所述池包含结合所述MHC / HLA分子的HCV-肽和不结合所述MHC / HLA分子的HCV-肽， - 用所述HCV-肽池接触所述MHC / HLA分子，由此HCV- 具有与所述MHC / HLA分子的结合能力的肽与所述MHC / HLA分子结合，形成包含所述HCV-肽和所述MHC / HLA分子的复合物， - 检测并任选地将所述复合物与所述HCV-肽分离 不结合所述MHC / HLA分子并任选地从所述复合物中分离和表征HCV-肽。

公开(公告)号：US20090081246A1

公开(公告)日：2009-03-26

申请号：US12112758

申请日：2008-04-30

申请人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

发明人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

IPC分类号： A61K39/00 ,  C12N5/00 ,  A61K35/12

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described are methods for isolating Hepatitis C Virus peptides (HPs) that have a binding capacity to a MHC/HLA molecule or a complex comprising the HCV-peptide and the MHC/HLA molecule. In one aspect, these methods include providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterizing the HCV-peptide from said complex. Also described are immunogenic compositions that stimulate a specific T cell response to hepatitis C virus when administered to a subject in an effective amount.

摘要翻译： 描述了分离具有对MHC / HLA分子的结合能力的丙型肝炎病毒肽（HP）或包含HCV-肽和MHC / HLA分子的复合物的方法。 一方面，这些方法包括提供一组HCV肽，所述池含有与所述MHC / HLA分子结合的HCV-肽和不结合所述MHC / HLA分子的HCV-肽，所述MHC / HLA分子 与所述HCV-肽库相结合，由此形成具有与所述MHC / HLA分子的结合能力的HCV肽与所述MHC / HLA分子结合，形成包含所述HCV-肽和所述MHC / HLA分子的复合物，检测和任选地 将所述复合物与不结合所述MHC / HLA分子的HCV-肽分离，并任选从所述复合物中分离和表征HCV-肽。 还描述了当以有效量施用于受试者时刺激特异性T细胞对丙型肝炎病毒的响应的免疫原性组合物。

公开(公告)号：US20060216691A1

公开(公告)日：2006-09-28

申请号：US10512885

申请日：2003-08-27

申请人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

发明人： Michael Buschle ,  Andre Habel ,  Christoph Klade ,  Frank Mattner ,  Oleksandr Otava ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Sandra Zinke ,  Helen Kirlappos

IPC分类号： C12Q1/70 ,  C07K14/18

CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/525 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/16122 ,  C12N2760/16122 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  Y10S930/223

摘要： Described is a method for isolating Hepatitis C Virus peptides (HPs) which have a binding capacity to a MHC/HLA molecule or a complex comprising said HCV-peptide and said MHC/HLA molecule characterized by the following steps: —providing a pool of HCV-peptide, said pool containing HCV-peptides which bind to said MHC/HLA molecule and HCV-peptides which do not bind to said MHC/HLA molecule, —contacting said MHC/HLA molecule with said pool of HCV-peptides whereby a HCV-peptide which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said HCV-peptide and said MHC/HLA molecule is formed, —detecting and optionally separating said complex from the HCV-peptide which do not bind to said MHC/HLA molecule and optionally isolating and characterising the HCV-peptide from said complex.

摘要翻译： 描述了分离具有与MHC / HLA分子的结合能力的丙型肝炎病毒肽（HP）或包含所述HCV-肽和所述MHC / HLA分子的复合物的方法，其特征在于以下步骤： - 提供HCV池 肽，所述池包含结合所述MHC / HLA分子的HCV-肽和不结合所述MHC / HLA分子的HCV-肽， - 用所述HCV-肽池接触所述MHC / HLA分子，由此HCV- 具有与所述MHC / HLA分子的结合能力的肽与所述MHC / HLA分子结合，形成包含所述HCV-肽和所述MHC / HLA分子的复合物， - 检测并任选地将所述复合物与所述HCV-肽分离 不结合所述MHC / HLA分子并任选地从所述复合物中分离和表征HCV-肽。

公开(公告)号：US20050221381A1

公开(公告)日：2005-10-06

申请号：US10505929

申请日：2003-02-27

申请人： Christof Klade ,  Juliane Schalich ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Max Birnstiel ,  Gerald Aichinger ,  Alexander Otava ,  Frank Mattner

发明人： Christof Klade ,  Juliane Schalich ,  Oresta Vytvytska ,  Wolfgang Zauner ,  Max Birnstiel ,  Gerald Aichinger ,  Alexander Otava ,  Frank Mattner

IPC分类号： C12Q1/02 ,  A61K35/12 ,  A61K38/00 ,  A61K39/00 ,  A61K39/245 ,  A61P31/12 ,  A61P43/00 ,  C07K7/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/045 ,  C07K16/28 ,  C07K14/74 ,  G01N33/53

CPC分类号： C07K7/06 ,  A61K2039/57 ,  C07K7/08 ,  C07K14/005 ,  C12N2710/16122 ,  G01N2500/04 ,  Y02A50/412

摘要： Described is a method for isolating ligands which have a binding capacity to a MHC/HLA molecule or a complex comprising said ligand and said MHC/HLA molecule which method comprises the following steps:—providing a pool of ligands, said pool containing ligands which bind to said MHC/HLA molecule and ligands which do not bind to said MHC/HLA molecule,—contacting said MHC/HLA molecule with said pool of ligands whereby a ligand which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said ligand and said MHC/HLA molecule is formed,—detecting and optionally separating said complex from the ligands which do not bind to said MHC/HLA molecule and—optionally isolating and characterising the ligand from said complex as well as a method for isolating T cell epitopes which have a binding capacity to a MHC/HLA molecule.

摘要翻译： 描述了分离对MHC / HLA分子具有结合能力的配体或包含所述配体和所述MHC / HLA分子的复合物的方法，该方法包括以下步骤： - 提供配体池，所述池含有结合的配体 对所述MHC / HLA分子和不结合所述MHC / HLA分子的配体， - 用所述配体池接触所述MHC / HLA分子，由此具有与所述MHC / HLA分子的结合能力的配体结合所述MHC / 形成HLA分子和包含所述配体和所述MHC / HLA分子的复合物， - 检测并任选地将所述复合物与不结合所述MHC / HLA分子的配体分离，并任选地从所述复合物中分离和表征配体 作为分离对MHC / HLA分子具有结合能力的T细胞表位的方法。

公开(公告)号：US20130034575A1

公开(公告)日：2013-02-07

申请号：US13613761

申请日：2012-09-13

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/00 ,  C07K16/00 ,  C07K7/08 ,  C07K14/00 ,  C07K7/06

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to auto-immunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: providing an antibody preparation, providing at least one expression library, identifying antigens which bind, screening the identified antigens, identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了一种鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型的疫苗 其特征在于以下步骤：提供抗体制剂，提供至少一种表达文库，鉴定结合筛选鉴定的抗原的抗原，鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，以及哪些 超免疫血清反应性抗原结合所述单独抗体的相关部分，任选分离所述超免疫血清反应性抗原，并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US20080038287A1

公开(公告)日：2008-02-14

申请号：US11614716

申请日：2006-12-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

IPC分类号： A61K39/00 ,  A61P43/00 ,  C07K14/00 ,  C07K16/18 ,  C12N15/00 ,  C12P21/04 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: —providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —screening said at least one expression library with said antibody preparation, —identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and —optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选，用来自具有抗体a的个体的来自个体血清的各抗体制剂筛选鉴定的抗原 所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主，鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合来自所述 单独血清，并且通过化学或重组方法选择性分离所述超免疫血清反应性抗原并产生所述超免疫血清反应性抗原。

公开(公告)号：US08323660B2

公开(公告)日：2012-12-04

申请号：US12853074

申请日：2010-08-09

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/085

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:—providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—screening said at least one expression library with said antibody preparation,—identifying antigens which bind in said screening to antibodies in said antibody preparation,—screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and—optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选，用来自具有抗体的个体的来自个体血清的单独抗体制剂筛选鉴定的抗原 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合 所述个体血清和任选地分离所述超免疫血清反应性抗原并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US07968297B2

公开(公告)日：2011-06-28

申请号：US10470048

申请日：2002-01-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： G01N33/53

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: —providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —screening said at least one expression library with said antibody preparation, —identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and —optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选， - 用来自个体的个体血清筛选鉴定的抗原与单独的抗体制剂 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的抗体， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原结合所述单个抗体制剂的相关部分 所述个体血清和 - 通过化学或重组方法选择性分离所述超免疫血清反应性抗原并产生所述超免疫血清反应性抗原。

公开(公告)号：US20110020402A1

公开(公告)日：2011-01-27

申请号：US12853074

申请日：2010-08-09

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/085 ,  C07K7/06 ,  C07K2/00 ,  A61K39/00 ,  C07K16/00 ,  C07K16/12 ,  G01N33/53 ,  A61P31/04 ,  A61P37/04

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:—providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogren, tumor, allergen or tissue or host prone to auto-immunity,—providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—screening said at least one expression library with said antibody preparation, identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and—optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清中提供抗体制剂 易于自身免疫， - 提供易于自身免疫的所述特定病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一个表达文库，鉴定结合所述抗体的抗原 对所述抗体制剂中的抗体进行筛选，从具有抗体的个体的个体血清中筛选鉴别的抗原 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合 所述个体血清和任选地分离所述超免疫血清反应性抗原并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US20050037444A1

公开(公告)日：2005-02-17

申请号：US10470048

申请日：2002-01-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

IPC分类号： G01N33/50 ,  A61K39/00 ,  A61K39/39 ,  A61K39/395 ,  A61P31/10 ,  B64C9/02 ,  C07K14/195 ,  C07K14/31 ,  C07K16/12 ,  C12N15/02 ,  C12N15/09 ,  C12N15/10 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  C40B40/02 ,  G01N33/15 ,  G01N33/53 ,  G01N33/574 ,  G01N33/554 ,  G01N33/569

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:—providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—screening said at least one expression library with said antibody preparation,—identifying antigens which bind in said screening to antibodies in said antibody preparation,—screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and—optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选，并用来自具有抗体的个体的来自个体血清的单独抗体制剂筛选鉴定的抗原 t表示特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主， - 识别所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合来自所述 单独血清和任选分离所述超免疫血清反应性抗原，并通过化学或重组方法产生所述超免疫血清反应性抗原。